Nanomaterials-based Polymerase Chain Reactions for DNA Detection

Dun      Pan; Yangqin      Wen; Lijuan      Mi; Chunhai      Fan; Jun      Hu

doi:10.2174/138527211794474447

Abstract

A range of nanometer-sized materials such as metal nanoparticles, semiconductor quantum dots, metal oxide nanostructures, carbon nanomaterials and polymer nanoparticles, have been introduced into polymerase chain reactions (PCR). They have shown to remarkably improve PCR amplification in sensitivity, specificity and extension rate. In this review, we will try to describe the development of nanomaterials-based PCR, including the types of nanomaterials, optimization of properties and mechanisms. In particular, several mechanisms have been introduced in view of special physical and chemical properties of nanomaterials.

Keywords: Nanomaterial, metal nanoparticle, QDs, metal oxide nanostructure, carbon nanomaterial, polymer, polymerase, DNA, PCR, DNA detection, optimization, specificity, sensitivity, extend rate, yields, hot-start, mechanism, walled carbon nanotubes (SWCNTs), gold nanoparticles, Silver nanoparticles, CdSe QDs, ZnO, Carbon nanotubes(CNTs), Carbon nanoparticles (CNPs), C60, poly(N-vinylpyrrolidone), Dendrimer, GC-rich efficiency, Japanese encephalitis virus (JEV), mosquito-borne neurotropic flavivirus, RT-PCR, Semiconductor Nanoparticles, Semiconductor quantum dots, mercaptoacetic, streptavidin, Metal Oxide Nanomaterials, walled carbon nanotubes (MWCNTs), carbon nanopowder, quantitative real-time, Nanometer-size Polymers, amphiphilic poly(N-vinylpyrrolidone) (PVP) copolymer, Copolymer 9, GC-rich -actin, pUC18 DNA, poly (amidoamine) (PAMAM) dendrimers, NANOPCR, scanning electron microscopy (SEM), croscopy (HRTEM), dithiothreitol (DTT), X-ray photoelectron spectroscopy (XPS)