Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150

Title: Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150

Volume: 13 Issue: 2

Author(s): Yusuke Kato, Yoriko Sawano and Masaru Tanokura

Affiliation:

Keywords: WW domain, air pump, reverse phase chromatography, surface plasmon resonance

Abstract: Production of GST-fused WW domains of FBP proteins was increased using the bubbling cultivation method for E. coli. Purified WW domains of FBP11 and FBP28 bound A PL motif peptide with dissociation constants (KD) of 248 ± 27 and 1880 ± 280 μM, respectively.

Cite this article as:

Kato Yusuke, Sawano Yoriko and Tanokura Masaru, Expression and Purification of Active WW Domains of FBP11/HYPA and FBP28/CA150, Protein & Peptide Letters 2006; 13 (2) . https://dx.doi.org/10.2174/092986606775101670

DOI https://dx.doi.org/10.2174/092986606775101670	Print ISSN 0929-8665
Publisher Name Bentham Science Publisher	Online ISSN 1875-5305