Abstract
Fluorescence-based molecular imaging in small animals is having a major impact on drug development and disease research and the ability to detect multiple molecular species at once is becoming increasingly important. Unlike bioluminescence, in fluorescence, ubiquitous autofluorescent signals from the skin need to be separated from that of labeled fluorophores to ensure proper quantitative data. Since its introduction in 2004, spectral imaging methods have become an important part of in vivo fluorescence imaging of small animals by enabling easy multiplexed imaging methods and through the quantitative removal of interfering skin autofluorescence signals. This article is a review of the literature on spectral imaging methods and applications in fluorescence imaging of small animals.
Keywords: Fluorescence, in vivo Imaging, Spectral Imaging, Autofluorescence Removal, Quantitation
Current Pharmaceutical Biotechnology
Title: Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals
Volume: 11 Issue: 6
Author(s): James R. Mansfield
Affiliation:
Keywords: Fluorescence, in vivo Imaging, Spectral Imaging, Autofluorescence Removal, Quantitation
Abstract: Fluorescence-based molecular imaging in small animals is having a major impact on drug development and disease research and the ability to detect multiple molecular species at once is becoming increasingly important. Unlike bioluminescence, in fluorescence, ubiquitous autofluorescent signals from the skin need to be separated from that of labeled fluorophores to ensure proper quantitative data. Since its introduction in 2004, spectral imaging methods have become an important part of in vivo fluorescence imaging of small animals by enabling easy multiplexed imaging methods and through the quantitative removal of interfering skin autofluorescence signals. This article is a review of the literature on spectral imaging methods and applications in fluorescence imaging of small animals.
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Cite this article as:
R. Mansfield James, Distinguished Photons: A Review of In Vivo Spectral Fluorescence Imaging in Small Animals, Current Pharmaceutical Biotechnology 2010; 11 (6) . https://dx.doi.org/10.2174/138920110792246474
DOI https://dx.doi.org/10.2174/138920110792246474 |
Print ISSN 1389-2010 |
Publisher Name Bentham Science Publisher |
Online ISSN 1873-4316 |
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