Applications of Carbon Based Electrodes for Voltammetric Determination of Lornoxicam in Pharmaceutical Dosage Form and Human Serum

Burcin      Bozal; Bengi      Uslu

doi:10.2174/1386207311004070599

Abstract

A simple, reliable and selective differential pulse (DP) and square wave (SW) voltammetric methods at glassy carbon (GC) and boron-doped diamond (BDD) electrodes of lornoxicam in pharmaceutical dosage form and in spiked human serum samples have been developed and evaluated. The possible oxidation mechanism was discussed. The voltammetric study of the model compounds allowed elucidating the possible oxidation mechanism of lornoxicam. The dependence of the peak current and peak potentials on pH, concentration, nature of the buffer, and scan rate were investigated. The oxidation of lornoxicam gave a single and irreversible peak at both electrodes. The process was found diffusion controlled. For glassy carbon electrode, the linearity of the calibration curve was obtained in the range of 4×10-7 M to 2×10-5 M for DPV method and 4×10-7 M to 4×10-5 M for SWV method in 0,1 M sulphuric acid solutions. Using boron-doped diamond electrode, the plot of the calibration curve was linear between 6×10-7 M and 1×10-4 M for both voltammetric methods in pH 2 BR buffer. The repeatability, reproducibility, precision and accuracy of the proposed methods were investigated.

Keywords: Lornoxicam, voltammetry, boron-doped diamond electrode, glassy carbon electrode, pharmaceutical dosage form, serum

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