Recombinant Expression in Moderate Halophiles

Masao      Tokunaga; Tsutomu      Arakawa; Hiroko      Tokunaga

doi:10.2174/138920110791112022

Abstract

A novel expression of recombinant proteins was developed using moderate halophiles that accumulate osmolytes and hence provide cytoplasmic environments where osmolyte-driven folding can take place. Promoters and selection marker were developed for high expression of foreign proteins. Examples are given for expression of bacterial nucleoside diphosphate kinase and human serine racemase.

Keywords: Soluble expression, moderate halophile, compatible solute, racemase, kinase

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Current Pharmaceutical Biotechnology

Title: Recombinant Expression in Moderate Halophiles

Volume: 11 Issue: 3

Author(s): Masao Tokunaga, Tsutomu Arakawa and Hiroko Tokunaga

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Keywords: Soluble expression, moderate halophile, compatible solute, racemase, kinase

Abstract: A novel expression of recombinant proteins was developed using moderate halophiles that accumulate osmolytes and hence provide cytoplasmic environments where osmolyte-driven folding can take place. Promoters and selection marker were developed for high expression of foreign proteins. Examples are given for expression of bacterial nucleoside diphosphate kinase and human serine racemase.

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Tokunaga Masao, Arakawa Tsutomu and Tokunaga Hiroko, Recombinant Expression in Moderate Halophiles, Current Pharmaceutical Biotechnology 2010; 11 (3) . https://dx.doi.org/10.2174/138920110791112022