Explore on the Mechanism of miRNA-146a/TAB1 in the Regulation of Cellular Apoptosis and Inflammation in Ulcerative Colitis Based on NF-κB Pathway

Title:Explore on the Mechanism of miRNA-146a/TAB1 in the Regulation of Cellular Apoptosis and Inflammation in Ulcerative Colitis Based on NF-κB Pathway

Volume: 24

Author(s): Xiaoying Xia, Qian Yang, Xue Han, Yulin Du, Shujun Guo, Mengqing Hua, Fang Fang, Zhigang Ma, Hua Ma, Hui Yuan, Wenjing Tian, Zebang Ding, Yanan Duan, Qi Huo and Yao Li*

Affiliation:

• Anhui Provincial Key Laboratory of Immunology in Chronic Diseases, Anhui Provincial Key Laboratory of Infection and Immunology, Department of Immunology, School of Laboratory Medicine, Bengbu Medical College, Bengbu 233030, China

Abstract: Objective: Ulcerative colitis (UC) is a chronic non-specific inflammatory disease of the rectum and colon with unknown etiology. A growing number of evidence suggest that the pathogenesis of UC is related to excessive apoptosis and production of inflammatory cytokines. However, the functions and molecular mechanisms associated with UC remain unclear.

Materials and Methods: The in vivo and in vitro models of UC were established in this study. MiRNA or gene expression was measured by qRT-PCR assay. ELISA, CCK-8, TUNEL, and flow cytometry assays were applied for analyzing cellular functions. The interactions between miR-146a and TAB1 were verified by luciferase reporter and miRNA pull-down assays.

Results: MiR-146a was obviously increased in UC patients, DSS-induced colitis mice, and TNF-ɑ-induced YAMC cells, when compared to the corresponding controls. MiR- 146a knockdown inhibited the inflammatory response and apoptosis in DSS-induced colitis mice and TNF-ɑ-induced YAMC cells. Mechanistically, we found that TAB1 was the target of miR-146a and miR-146a knockdown suppressed the activation of NF-κB pathway in UC. More importantly, TAB1 could overturn the inhibitory effect of antagomiR-146a on cell apoptosis and inflammation in UC.

Conclusion: MiR-146a knockdown inhibited cell apoptosis and inflammation via targeting TAB1 and suppressing NF-κB pathway, suggesting that miR-146a may be a new therapeutic target for UC treatment.

Cite this article as:

Xia Xiaoying, Yang Qian, Han Xue, Du Yulin, Guo Shujun, Hua Mengqing, Fang Fang, Ma Zhigang, Ma Hua, Yuan Hui, Tian Wenjing, Ding Zebang, Duan Yanan, Huo Qi and Li Yao*, Explore on the Mechanism of miRNA-146a/TAB1 in the Regulation of Cellular Apoptosis and Inflammation in Ulcerative Colitis Based on NF-κB Pathway, Current Molecular Medicine 2024; 24 () . https://dx.doi.org/10.2174/0115665240273807231122052445