The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines

Title:The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines

Volume: 20

Author(s): Mohammad Hossein Golestani Poor, Shohreh Zare Karizi, Seyed Ali Mirhosseini, Mohammad Javad Motamedi, Fateme Frootan, Soghra Khani and Jafar Amani*

Affiliation:

• Applied Microbiology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract:

Background: The use of targeted therapy has been increasing for cancer treatment. The aim of this study is to investigate chitosan-based ricin-Herceptin (rh) immunotoxin on breast cancer cell lines.

Methods: The gene construct encoding immunotoxin was designed, cloned, and expressed in E. coli BL21 (DE3). The expressed proteins were isolated by the nickel-nitrilotriacetic acid column and were analyzed by the Western-blotting. The cytotoxicity of immunotoxin was assayed on breast cell line MCF-7 and using MTT assay at 24 and 48 h treatment.

Results: The immunotoxins extrication rate, size, loading percentage, and electric charge of nanoparticles were reported appropriately as 78%, 151.5 nm, 83.53%, and +11.1 mV, respectively. The encapsulated immunotoxins led to the death of 70% and 78% of MCF-7 cells at 24 and 48 h treatment, respectively. The noncapsulated counterparts at equal doses killed 53% and 62% of cancer cells at the same time points.

Conclusion: The chitosan-immunotoxins impose potential cytotoxic effects on cancer cells.

Cite this article as:

Golestani Poor Mohammad Hossein, Zare Karizi Shohreh, Mirhosseini Seyed Ali, Motamedi Mohammad Javad, Frootan Fateme, Khani Soghra and Amani Jafar*, The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines, Current Nanoscience 2024; 20 () . https://dx.doi.org/10.2174/0115734137278545240102055626