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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Factors Affecting the Micropropagation of Sapindus trifoliatus from Nodal Explants of Mature Tree

Author(s): Pooja Asthana, Manoj K. Rai* and Uma Jaiswal

Volume 13, Issue 1, 2024

Published on: 17 January, 2024

Page: [58 - 67] Pages: 10

DOI: 10.2174/0122115501289941240109061526

Price: $65

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Abstract

Background: Explant recalcitrance is one of the major challenges during the tissue culture of a tree species, especially when explants are derived from mature plants. It is mainly associated with the genotypes/species, source and physiological status of explants, seasonal variations, and competency/incompetency of explants towards PGRs for in vitro manipulations. Therefore, to optimize the appropriate conditions and minimize explant recalcitrance, it is necessary to elucidate the different factors influencing the tissue culture of a tree species.

Objective: Several factors influencing in vitro shoot induction from nodal explants derived from mature plants of Sapindus trifoliatus were investigated.

Methods: Nodal segment obtained from mature plants was used as an explant for shoot regeneration. The factors assessed in this study included the types and concentrations of plant growth regulators, nutrient composition of MS (Murashige and Skoog) medium, sucrose concentrations, the position of nodes on their respective branches, seasonal variations, and successive transfers of mother explant.

Results: The performance of nodal explants was better on modified MS medium, i.e., M4 medium (half-strength of MS major salts and full-strengths of MS minor salts, Fe-EDTA and MS organic nutrients each) than the full-strength MS medium. The addition of BAP in the medium resulted in a higher shoot induction rate than other cytokinins i.e., Kin, Zeatin, TDZ. M4 medium supplemented with 3.0 mg l-1 BAP and 2% sucrose was optimum for shoot proliferation. However, elongation of shoots was observed only when induced shoots, along with the mother explant, were subcultured on a medium containing 1.0 mg l-1 BAP. The shoot multiplication was positively affected by the repeated transfer of the mother explant for different passages on the multiplication medium. Mother explant transferred to multiplication medium (0.8% agar-solidified M4 medium + 2% sucrose + 1.0 mg l-1 BAP) proliferated maximum shoots after fourth passages. Regenerated shoots were rooted in vitro and further successfully acclimatized in field conditions.

Conclusion: The in vitro regeneration system developed from an explant obtained from mature trees can be successfully exploited for mass multiplication of Sapindus trifoliatus, a medicinally and commercially important tree.

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[1]
Benson EE. In vitro recalcitrance: An introduction. In Vitro Cell Dev Biol Plant 2000; 36: 141-8.
[http://dx.doi.org/10.1007/s11627-000-0029-z]
[2]
Kalia RK, Rai MK, Sharma R, Bhatt RK. Understanding Tecomella undulata: An endangered pharmaceutically important timber species of hot arid regions. Genet Resour Crop Evol 2014; 61(7): 1397-421.
[http://dx.doi.org/10.1007/s10722-014-0140-3]
[3]
Arora K, Rai MK, Sharma AK. Tissue culture mediated biotechnological interventions in medicinal trees: Recent progress. Plant Cell Tissue Organ Cult 2022; 150(2): 267-87.
[http://dx.doi.org/10.1007/s11240-022-02298-1]
[4]
George EF, Hall MA, Jan De Klerk G. Plant propagation by tissue culture. (3rd ed.), Dordrecht: Springer 2008.
[5]
Bonga JM. Can explant choice help resolve recalcitrance problems in in vitro propagation, a problem still acute especially for adult conifers? Trees 2017; 31(3): 781-9.
[http://dx.doi.org/10.1007/s00468-016-1509-z]
[6]
Patel P, Sarswat SK, Modi A. Strategies to overcome explant recalcitrance under in vitro conditions. In: Rai AC, Kumar A, Modi A, Singh M, Eds. Advances in plant tissue culture: Current developments and future trends. U.K.: Elsevier 2022; pp. 283-94.
[http://dx.doi.org/10.1016/B978-0-323-90795-8.00003-5]
[7]
Rai MK, Asthana P, Jaiswal VS, Jaiswal U. Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees 2010; 24(1): 1-12.
[http://dx.doi.org/10.1007/s00468-009-0384-2]
[8]
Kher MM, Nataraj M, Arun Kumar AN, et al. Tissue culture of Indian rosewood (Dalbergia latifolia Roxb.). Biologia 2021; 76(12): 3595-604.
[http://dx.doi.org/10.1007/s11756-021-00914-7]
[9]
Mahar KS, Rana TS, Ranade SA, Pande V, Palni LMS. Estimation of genetic variability and population structure in Sapindus trifoliatus L., using DNA fingerprinting methods. Trees 2013; 27(1): 85-96.
[http://dx.doi.org/10.1007/s00468-012-0770-z]
[10]
Kirtikar KR, Basu BD. Indian medicinal plants 1. New Delhi: International Book Distributors 1999; pp. 632-5.
[11]
Singh R, Sharma B. Pharmacological activities and medicinal implications of Sapindus spp. In: Biotechnological advances, phytochemical analysis and ethnomedical implications of Sapindus species. Springer 2019; pp. 17-22.
[http://dx.doi.org/10.1007/978-981-32-9189-8_6]
[12]
Asthana P, Rai MK, Jaiswal U. Somatic embryogenesis from sepal explants in Sapindus trifoliatus, a plant valuable in herbal soap industry. Ind Crops Prod 2017; 100: 228-35.
[http://dx.doi.org/10.1016/j.indcrop.2017.02.034]
[13]
Desai HV, Bhatt PN, Mehta AR. Plant regeneration of Sapindus trifoliatus L. (soapnut) through somatic embryogenesis. Plant Cell Rep 1986; 5(3): 190-1.
[http://dx.doi.org/10.1007/BF00269115] [PMID: 24248129]
[14]
Asthana P, Rai MK, Jaiswal U. In vitro selection, regeneration and characterization of NaCl-tolerant plants of Sapindus trifoliatus: An important multipurpose tree. Plant Cell Tissue Organ Cult 2023; 154(2): 227-38.
[http://dx.doi.org/10.1007/s11240-023-02552-0]
[15]
Asthana P, Jaiswal VS, Jaiswal U. Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiol Plant 2011; 33(5): 1821-9.
[http://dx.doi.org/10.1007/s11738-011-0721-0]
[16]
Murashige T, Skoog F. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 1962; 15(3): 473-97.
[http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x]
[17]
Malik SK, Chaudhury R, Kalia RK. Rapid in vitro multiplication and conservation of Garcinia indica: A tropical medicinal tree species. Sci Hortic 2005; 106(4): 539-53.
[http://dx.doi.org/10.1016/j.scienta.2005.05.002]
[18]
Tripathi M, Kumari N. Micropropagation of a tropical fruit tree Spondias mangifera Willd. through direct organogenesis. Acta Physiol Plant 2010; 32(5): 1011-5.
[http://dx.doi.org/10.1007/s11738-010-0484-z]
[19]
Arora K, Sharma M, Srivastava J, Ranade SA, Sharma AK. Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments. Agrofor Syst 2010; 78(1): 53-63.
[http://dx.doi.org/10.1007/s10457-009-9230-1]
[20]
Phulwaria M, Rai MK. Harish, Gupta AK, Ram K, Shekhawat NS. An improved micropropagation of Terminalia bellirica from nodal explants of mature tree. Acta Physiol Plant 2012; 34(1): 299-305.
[http://dx.doi.org/10.1007/s11738-011-0828-3]
[21]
Rathore JS, Rai MK, Phulwaria M, Shekhawat NS. A liquid culture system for improved micropropagation of mature Acacia nilotica (L.) Del. ssp. indica and ex vitro rooting. Proc Natl Acad Sci, India, Sect B Biol Sci 2014; 84(1): 193-200.
[http://dx.doi.org/10.1007/s40011-013-0204-8]
[22]
Vibha JB, Shekhawat NS, Mehandru P, Dinesh R. Rapid multiplication of Dalbergia sissoo Roxb.: A timber yielding tree legume through axillary shoot proliferation and ex vitro rooting. Physiol Mol Biol Plants 2014; 20(1): 81-7.
[http://dx.doi.org/10.1007/s12298-013-0213-3] [PMID: 24554841]
[23]
Gupta AK. Harish, Rai MK, Phulwaria M, Agarwal T, Shekhawat NS. In vitro propagation, encapsulation, and genetic fidelity analysis of Terminalia arjuna: A cardioprotective medicinal tree. Appl Biochem Biotechnol 2014; 173(6): 1481-94.
[http://dx.doi.org/10.1007/s12010-014-0920-4] [PMID: 24817511]
[24]
Patel AK, Lodha D, Shekhawat NS. An improved micropropagation protocol for the ex situ conservation of Mitragyna parvifolia (Roxb.) Korth. (Rubiaceae): An endangered tree of pharmaceutical importance. In Vitro Cell Dev Biol Plant 2020; 56(6): 817-26.
[http://dx.doi.org/10.1007/s11627-020-10089-6] [PMID: 32837138]
[25]
Machado JS, Degenhardt J, Maia FR, Quoirin M. Micropropagation of Campomanesia xanthocarpa O. Berg (myrtaceae), a medicinal tree from the brazilian atlantic forest. Trees 2020; 34(3): 791-9.
[http://dx.doi.org/10.1007/s00468-020-01958-z]
[26]
Iiyama CM, Cardoso JC. Micropropagation of Melaleuca alternifolia by shoot proliferation from apical segments. Trees 2021; 35(5): 1497-509.
[http://dx.doi.org/10.1007/s00468-021-02131-w]
[27]
Singh R, Rai MK, Kumari N. Somatic embryogenesis and plant regeneration in Sapindus mukorossi Gaertn. from leaf-derived callus induced with 6-benzylaminopurine. Appl Biochem Biotechnol 2015; 177(2): 498-510.
[http://dx.doi.org/10.1007/s12010-015-1758-0] [PMID: 26208689]
[28]
Marks TR, Simpson SE. Factors affecting shoot development in apically dominant Acer cultivars in vitro. J Hortic Sci 1994; 69(3): 543-37.
[http://dx.doi.org/10.1080/14620316.1994.11516486]
[29]
Rai MK, Jaiswal VS, Jaiswal U. Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of In vitro raised plantlets. J Fruit Ornam Plant Res 2009; 17: 29-38.
[30]
Vengadesan G, Ganapathi A, Amutha S, Selvaraj N. In vitro propagation of Acacia species—a review. Plant Sci 2002; 163(4): 663-71.
[http://dx.doi.org/10.1016/S0168-9452(02)00144-9]
[31]
Rathore J, Rathore V, Shekhawat NS, et al. Micropropagation of woody plants. In: Srivastava P, Narula A, Srivastava S, Eds. Plant biotechnology and molecular markers. Dordrecht: Springer 2004; pp. 195-205.
[32]
Mallikarjuna K, Rajendrudu G. High frequency in vitro propagation of Holarrhena antidysenterica from nodal buds of mature tree. Biol Plant 2007; 51(3): 525-9.
[http://dx.doi.org/10.1007/s10535-007-0112-y]
[33]
Pandey S, Singh M, Jaiswal U, Jaiswal VS. Shoot initiation and multiplication from a mature tree of Terminalia arjuna roxb. In Vitro Cell Dev Biol Plant 2006; 42(5): 389-93.
[http://dx.doi.org/10.1079/IVP2006790]
[34]
Jain N, Babbar SB. Effect of carbon source on the shoot proliferation potential of epicotyl explants of Syzygium cuminii. Biol Plant 2003; 46(1): 133-6.
[http://dx.doi.org/10.1023/A:1027305604113]
[35]
Saxena S. In vitro propagation of the bamboo (Bambusa tulda Roxb.) through shoot proliferation. Plant Cell Rep 1990; 9(8): 431-4.
[http://dx.doi.org/10.1007/BF00232266] [PMID: 24227171]
[36]
Singh M, Jaiswal U, Jaiswal VS. Thidiazuron-induced shoot multiplication and plant regeneration in bamboo (Dendrocalamus strictus Nees). J Plant Biochem Biotechnol 2001; 10(2): 133-7.
[http://dx.doi.org/10.1007/BF03263122]
[37]
Purohit SD, Tak K. In vitro propagation of an adult tree Foreonia limonia L. through axillary branching. Indian J Exp Biol 1992; 30: 377-9.
[38]
Saxena S, Bhojwani SS. In vitro clonal multiplication of 4-year-old plants of the bamboo,Dendrocalamus longispathus kurz. In Vitro Cell Dev Biol Plant 1993; 29(3): 135-42.
[http://dx.doi.org/10.1007/BF02632285]
[39]
Husain MK, Anis M. Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree). Acta Physiol Plant 2009; 31(4): 765-72.
[http://dx.doi.org/10.1007/s11738-009-0290-7]
[40]
Amin MN, Jaiswal VS. Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tissue Organ Cult 1987; 9(3): 235-43.
[http://dx.doi.org/10.1007/BF00040809]
[41]
Chhajer S, Kalia RK. Seasonal and micro-environmental factors controlling clonal propagation of mature trees of marwar teak [Tecomella undulata (Sm.) Seem]. Acta Physiol Plant 2017; 39(2): 60.
[http://dx.doi.org/10.1007/s11738-017-2364-2]
[42]
Shekhawat JK, Rai MK, Shekhawat NS, Kataria V. Synergism of m-topolin with auxin and cytokinin enhanced micropropagation of Maytenus emarginata. In Vitro Cell Dev Biol Plant 2021; 57(3): 418-26.
[http://dx.doi.org/10.1007/s11627-020-10132-6]
[43]
Shukla S, Shukla SK, Mishra SK. In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: A medicinal tree. Trees 2009; 23(2): 409-13.
[http://dx.doi.org/10.1007/s00468-008-0290-z]
[44]
Patel AK, Phulwaria M, Rai MK, Gupta AK, Shekhawat S, Shekhawat NS. In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth. & Hook. f.: An endemic and endangered edible plant species of the Thar Desert. Sci Hortic 2014; 165: 175-80.
[http://dx.doi.org/10.1016/j.scienta.2013.10.039]

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