Network Pharmacology and Experimental Validation Explore the
Pharmacological Mechanisms of Herb Pair for Treating Rheumatoid
Arthritis

Xi-Xi      Xu; Hua      Shao; Qiao-Xue      Wang; Zi-Yuan      Wang
doi:10.2174/0113862073263839231129163200
Abstract

Objective: This study aimed to elucidate the multitarget mechanism of the Mori Ramulus - Taxilli Herba (MT) herb pair in treating rheumatoid arthritis (RA).
Methods: The targets of the herb pair and RA were predicted from databases and screened through cross-analysis. The core targets were obtained using protein-protein interaction (PPI) network analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed. Finally, animal experiments were conducted to validate the anti-RA effect and mechanism of this herb pair.
Results: This approach successfully identified 9 active compounds of MT that interacted with 6 core targets (AKT1, TNF, IL6, TP53, VEGFA, and IL1β). Pathway and functional enrichment analyses revealed that MT had significant effects on the TNF and IL-17 signaling pathways. The consistency of interactions between active components and targets in these pathways was confirmed through molecular docking. Moreover, the potential therapeutic effect of MT was verified in vivo, demonstrating its ability to effectively relieve inflammation by regulating these targeted genes and pathways.
Conclusion: The present work suggests that the therapeutic effect of MT herb pair on RA may be attributed to its ability to regulate the TNF signaling pathway and IL-17 signaling pathway.
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Graphical Abstract

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DOI https://dx.doi.org/10.2174/0113862073263839231129163200	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402
Combinatorial Chemistry & High Throughput Screening

Network Pharmacology and Experimental Validation Explore the Pharmacological Mechanisms of Herb Pair for Treating Rheumatoid Arthritis

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