Login Register Cart 0
Generic placeholder image

Recent Patents on Biotechnology

Editor-in-Chief

ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

Back
Journal Home About Journal Editorial Board Journal Insight Current Issue Volumes/Issues
Subscribe
Research Article

The Anti-ulcer Potential of Weissella cibaria Assisted Bio-fermented Product of Citrus limetta Waste Peel in Wistar Albino Rats

Author(s): Monika Singh, Shreshtha Singh, Dinesh Puri*, Shalini Kapoor Sawhney, Nitin Kumar, Mohd. Yasir and Pankaj Nainwal

Volume 18, Issue 4, 2024

Published on: 29 November, 2023

Page: [344 - 357] Pages: 14

DOI: 10.2174/0118722083278152231121173605

Price: $65

Abstract

Background: There are patents available related to fermented food and beverages which enhance to human health. Citrus limetta (Mosambi) has a high content of flavonoids and exhibits antioxidant activity, which could stimulate the digestive system and be useful for gastroprotective activity. It supports digestion by neutralizing the acidic digestive juices and reducing gastric acidity.

Objective: This study explored the potential of using waste peel extract from Citrus limetta to prevent ulcers. The study specifically sought to assess the anti-ulcer properties of fermented and non-fermented extracts and compare them. Further, the study looked at the potential benefits of treating or preventing ulcers with Citrus limetta waste peels and whether fermentation affected the efficacy of the treatment.

Methods: Thirty female Wistar albino rats were equally distributed into five different groups. Group 1 received distilled water (20 ml/kg/b.w); Group 2 received indomethacin (mg/kg/b.w); Group 3 received omeprazole (20 mg/kg/b.w); Group 4 received aqueous extract of Mosambi peel (400 mg/kg/b.w) and Group 5 received fermented product of extract of Mosambi peel (400 mg/kg/b.w).

Results: Findings explored that, compared to non-fermented citrus fruit juice, biofermented exhibited less gastric volume (1.58 ± 0.10 ml vs. 1.8 ± 0.14 ml), reduced MDA levels (355.23 ± 100.70 μmol/mg protein vs. 454.49 ± 155.88 μmol/mg protein), and low ulcer index (0.49 ± 0.07 vs. 0.72 ± 0.14).

Conclusion: The results suggest that the bio-fermented product of Citrus limetta peel has better anti-ulcer potential against peptic ulcer induced by indomethacin in Wistar albino rats compared to non-fermented.

« Previous Next »
Graphical Abstract

[1]
Karthikeyan S, Gobianand K. Antiulcer activity of ethanol leaf extract of Cassia fistula. Pharm Biol 2010; 48(8): 869-77.
[http://dx.doi.org/10.3109/13880200903302838] [PMID: 20673173]
[2]
Kommavari CS, Bandaru N, Shaik M, Konduri P. Anti-ulcer activity of methanolic extract of Polycarpea aurea in asprin plus pylorus ligation- induced ulcer model in rats. J Pharm Sci 2019; 11: 5.
[3]
Vimala G, Gricilda Shoba F. A review on antiulcer activity of few Indian medicinal plants. Int J Microbiol 2014; 2014: 1-14.
[http://dx.doi.org/10.1155/2014/519590] [PMID: 24971094]
[4]
Yismaw YE, Abdelwuhab M, Ambikar B. Phytochemical and antiulcer activity screening of seed extract of Cordia Africana Lam (Boraginaceae) in pyloric ligated rats. CPAA 2020; 12: 67-73.
[http://dx.doi.org/10.2147/CPAA.S245672] [PMID: 32636685]
[5]
Khan AA, Mahmood T, Siddiqui HH, Akhtar J. Phytochemical and pharmacological properties on Citrus limetta (Mosambi). J Chem Pharm Res 2016; 8(3): 555-63.
[6]
Dasaroju S, Gottumukkala K M. Current trends in the research of Emblica officinalis (Amla): A pharmacological perspective. Int J Pharm Sci Rev Res 2014; 24(2): 150-9.
[7]
Bigoniya P, Singh K. Ulcer protective potential of standardized hesperidin, a citrus flavonoid isolated from Citrus sinensis. Rev Bras Farmacogn 2014; 24(3): 330-40.
[http://dx.doi.org/10.1016/j.bjp.2014.07.011]
[8]
Zia-ur-Rehman . Citrus peel extract-a natural source of antioxidant. Food Chem 2006; 99(3): 450-4.
[http://dx.doi.org/10.1016/j.foodchem.2005.07.054]
[9]
Benavente-García O, Castillo J, Marin FR, Ortuño A, Del Río JA. Uses and properties of Citrus flavonoids. J Agric Food Chem 1997; 45(12): 4505-15.
[http://dx.doi.org/10.1021/jf970373s] [PMID: 18593176]
[10]
Sing S, Singh M, Puri D. A review on therapeutic effect of non bio-fermented and bio-fermented product of various herbal drugs in the treatment of gastric ulcer. Letters in App NanoBioSci 2020; 9(2): 1042-8.
[http://dx.doi.org/10.33263/LIANBS92.10421048]
[11]
Lim JM, Song CH, Park SJ, et al. Protective effects of triple fermented barley extract (FBe) on indomethacin-induced gastric mucosal damage in rats. BMC Complement Altern Med 2019; 19(1): 49.
[http://dx.doi.org/10.1186/s12906-019-2457-0] [PMID: 30786935]
[12]
Rodríguez C, Medici M, Mozzi F, Font de Valdez G. Therapeutic effect of Streptococcus thermophilus CRL 1190-fermented milk on chronic gastritis. World J Gastroenterol 2010; 16(13): 1622-30.
[http://dx.doi.org/10.3748/wjg.v16.i13.1622] [PMID: 20355240]
[13]
Oh WS, Jung JC, Choi YM, Mun JY, Ku SK, Song CH. Protective effects of fermented rice extract on ulcerative colitis induced by dextran sodium sulfate in mice. Food Sci Nutr 2020; 8(3): 1718-28.
[http://dx.doi.org/10.1002/fsn3.1460] [PMID: 32180979]
[14]
Ikpeazu O, Elekwa I, Ugbogu AE, Arunsi U, Uche-Ikonne C. Preliminary evaluation of anti-ulcer potential of aqueous extract of fermented unripe Musa paradisiaca in wistar rats. Available from: https://www.semanticscholar.org/paper/Preliminary-Evaluation-of-Anti-ulcer-Potential-of-Ikpeazu-Elekwa/1251b43ad4ef0500f6ad84f6e6b0a222e7a1a119 (Accessed on: 2023-10-01).
[15]
Azwanid NN. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4(196): 2167-412.
[http://dx.doi.org/10.4172/2167-0412.1000196]
[16]
Gokhale SB, Purohit AP, Kokate CK. Pathway to Screen Phytochemical Nature of Natural Drugs: Pharmcognosy: Pune: Nirali: Prakashan 2008.
[17]
Carlosama Adriana M, Rodríguez Misael C, Londoño Guillermo C, Sánchez Fernando O, Cock Liliana S. Optimization of the reproduction of Weissella cibaria in a fermentation substrate formulated with agroindustrial waste. Biotechnol Rep 2021; 32: e00671.
[http://dx.doi.org/10.1016/j.btre.2021.e00671] [PMID: 34603976]
[18]
Gaur PK, Kumar S S, Puri D. Antidiabetic potential and metabolite profiling of biotransformed polyherbal extract using Wickerhamomyces anomalus strain (MTCC-4133). Process Biochem 2021; 102: 199-206.
[http://dx.doi.org/10.1016/j.procbio.2020.12.020]
[19]
Sofidiya MO, Bamigbade O, Basheeru K, Adegoke O, Agunbiade FO. Chemical composition, gastroprotective, and antioxidant activities of Schrebera arborea fruits. J Herbs Spices Med Plants 2020; 26(1): 89-100.
[http://dx.doi.org/10.1080/10496475.2019.1679691]
[20]
Jacquier M, Crauste F, Soulage CO, Soula HA. A predictive model of the dynamics of body weight and food intake in rats submitted to caloric restrictions. PLoS One 2014; 9(6): e100073.
[http://dx.doi.org/10.1371/journal.pone.0100073] [PMID: 24932616]
[21]
Abebaw M, Mishra B, Gelayee DA. Evaluation of anti-ulcer activity of the leaf extract of Osyris quadripartita Decne. (Santalaceae) in rats. J Exp Pharmacol 2017; 9: 1-11.
[http://dx.doi.org/10.2147/JEP.S125383] [PMID: 28144167]
[22]
Satish N, Nisha KS, Nagesh HS. Evaluation of antiulcer activity of hydroalcoholic fruit pulp extract of Cucumis Sativus. Int J Pharm Sci Res 2015; 6(11): 4712-20.
[23]
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
[http://dx.doi.org/10.1016/0003-2697(79)90738-3] [PMID: 36810]
[24]
Lemos LMS, Martins TB, Tanajura GH, et al. Evaluation of antiulcer activity of chromanone fraction from Calophyllum brasiliesnse Camb. J Ethnopharmacol 2012; 141(1): 432-9.
[http://dx.doi.org/10.1016/j.jep.2012.03.006] [PMID: 22425905]
[25]
Bagheri SM, Zare-Mohazabieh F, Momeni-Asl H, Yadegari M, Mirjalili A, Anvari M. Antiulcer and hepatoprotective effects of aqueous extract of Plantago ovata seed on indomethacin-ulcerated rats. Biomed J 2018; 41(1): 41-5.
[http://dx.doi.org/10.1016/j.bj.2018.01.001] [PMID: 29673551]
[26]
Chon H, Kim G, Kim S. Comparison of aqueous plant extracts before and after fermentation with Lactobacillus paracasei LS-2 on cytokine induction and antioxidant activity. Nat Prod Commun 2010; 5(8): 1934578X1000500.
[http://dx.doi.org/10.1177/1934578X1000500827] [PMID: 20839635]
[27]
Choi SY, Hwang JH, Park SY, et al. Fermented guava leaf extract inhibits LPS-induced COX-2 and iNOS expression in Mouse macrophage cells by inhibition of transcription factor NF-κB. Phytother Res 2008; 22(8): 1030-4.
[http://dx.doi.org/10.1002/ptr.2419] [PMID: 18618521]
[28]
Nair V. Fermented soy nutritional supplements including mushroom components. US20110206721A1; 2011.
[29]
Lu KM. Methods for inhibiting cancer growth, reducing infection and promoting general health with a fermented soy extract. US6855350B2; 2005.

Rights & Permissions Print Cite
© 2024 Bentham Science Publishers | Privacy Policy