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Current Aging Science

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ISSN (Print): 1874-6098
ISSN (Online): 1874-6128

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Research Article

Mesenchymal Stromal Cells Nanovesicles Carry microRNA with Nephroprotective Proprieties Regardless of Aging

Author(s): Marcia Bastos Convento*, Andréia Silva de Oliveira, Mirian Aparecida Boim and Fernanda Teixeira Borges

Volume 17, Issue 2, 2024

Published on: 14 November, 2023

Page: [118 - 126] Pages: 9

DOI: 10.2174/0118746098272926231107061047

Price: $65

Abstract

Containing information molecules from their parent cells and inclining to fuse with targeted cells, bone marrow mesenchymal stromal cells-derived extracellular vesicles (MSCs- EV) are valuable in nanomedicine.

Background: The effects of aging on the paracrine mechanism and in the production and action of MSCs-EV and their cargos of miR-26a and siRNA-26a for the treatment of tubular renal cells under nephrotoxicity injury remain unelucidated.

Objective: The purpose of this study was to evaluate MSCs-EV of different ages and their ability to deliver the cargos of miR-26a and siRNA-26a to target renal tubular cells affected by nephrotoxicity injury.

Methods: In a model of gentamicin-induced nephrotoxicity, renal tubular cells treated with MSCs-EV expressing or not expressing microRNA-26a were analyzed. Western blotting was utilized to evaluate cell cycle markers, and MTT assay was utilized to evaluate auto-renovation capacity.

Results: Tubular cells under nephrotoxicity injury showed decreased proliferative capacity, but the treatment in the tubular renal cells under nephrotoxicity injury with MSCs-EV expressing microRNA-26a showed nephroprotective effects, regardless of EV age. While the treatment with EV-mediated siRNA-26a failed to preserve the nephroprotective effects equally, regardless of age.

Conclusion: Mesenchymal stromal cell nanovesicles carry microRNA with nephroprotective proprieties regardless of aging.

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