Abstract
Background: Astragalus, as a common traditional Chinese medicine (TCM), has many pharmacological effects, such as protection of nerves, heart, blood vessels, kidneys, etc. It can also be used to treat colds, numbness, diarrhea, indigestion, and anemia. Because of the current market, there are a large number of synonyms, synonyms, and multiple sources of one medicine. The use of near-edge plants in medicine and other fake, substandard as a good phenomenon also prevails.
Methods: In this study, a method combining electrochemical fingerprinting with SPSS software was developed to identify the origin of Astragalus membranaceus. The electrochemical fingerprints of Astragalus membranaceus with different accessions and different origins were examined using an electrochemical workstation. Then the characteristic parameters were selected to achieve the origin identification of Astragalus with SPSS software using cluster analysis and discriminant analysis.
Results: The results of this method divided the origin of Astragalus into the Qinling production area and other production areas
Conclusion: This method can be used to identify the origin of Astragalus membranaceus, which provides a new method to identify Astragalus membranaceus and other TCM without extracting the active ingredients of Chinese medicines, in line with the traditional "holistic" treatment concept of TCM.
Graphical Abstract
[1]
Zeng, P.; Li, J.; Chen, Y.; Zhang, L. The structures and biological functions of polysaccharides from traditional Chinese herbs. Prog. Mol. Biol. Transl. Sci., 2019, 163, 423-444.
[http://dx.doi.org/10.1016/bs.pmbts.2019.03.003] [PMID: 31030757]
[http://dx.doi.org/10.1016/bs.pmbts.2019.03.003] [PMID: 31030757]
[2]
Su, H.; Shaker, S.; Kuang, Y.; Zhang, M.; Ye, M.; Qiao, X. Phytochemistry and cardiovascular protective effects of Huang‐Qi (Astragali Radix). Med. Res. Rev., 2021, 41(4), 1999-2038.
[http://dx.doi.org/10.1002/med.21785] [PMID: 33464616]
[http://dx.doi.org/10.1002/med.21785] [PMID: 33464616]
[3]
Ny, V.; Houška, M.; Pavela, R.; Tříska, J. Potential benefits of incorporating Astragalus membranaceus into the diet of people undergoing disease treatment: An overview. J. Funct. Foods, 2021, 77, 104339-104348.
[http://dx.doi.org/10.1016/j.jff.2020.104339]
[http://dx.doi.org/10.1016/j.jff.2020.104339]
[4]
Li, S.S.; Sun, Y.; Huang, J.; Wang, B.; Gong, Y.N.; Fang, Y.X.; Liu, Y.Y.; Wang, S.; Guo, Y.; Wang, H.; Xu, Z.F.; Guo, Y.M. Anti-tumor effects and mechanisms of Astragalus membranaceus (AM) and its specific immunopotentiation. J. Ethnopharmacol., 2020, 258, 112797-112805.
[http://dx.doi.org/10.1016/j.jep.2020.112797] [PMID: 32243990]
[http://dx.doi.org/10.1016/j.jep.2020.112797] [PMID: 32243990]
[5]
Zhang, W.; Cui, Y.; Liu, Z.; Wang, S.; Yang, A.; Li, X.; Zhang, J. Astragalus membranaceus ultrafine powder alleviates hyperuricemia by regulating the gut microbiome and reversing bile acid and adrenal hormone biosynthesis dysregulation. Arab. J. Chem., 2022, 15(9), 103970-103983.
[http://dx.doi.org/10.1016/j.arabjc.2022.103970]
[http://dx.doi.org/10.1016/j.arabjc.2022.103970]
[6]
Sheik, A.; Kim, K.; Varaprasad, G.L.; Lee, H.; Kim, S.; Kim, E.; Shin, J.Y.; Oh, S.Y.; Huh, Y.S. The anti-cancerous activity of adaptogenic herb Astragalus membranaceus. Phytomedicine, 2021, 91, 153698-153711.
[http://dx.doi.org/10.1016/j.phymed.2021.153698] [PMID: 34479785]
[http://dx.doi.org/10.1016/j.phymed.2021.153698] [PMID: 34479785]
[7]
Zhang, J.L.; Li, W.X.; Li, Y.; Wong, M.S.; Wang, Y.J.; Zhang, Y. Therapeutic options of TCM for organ injuries associated with COVID-19 and the underlying mechanism. Phytomedicine, 2021, 85, 153297-153304.
[http://dx.doi.org/10.1016/j.phymed.2020.153297] [PMID: 32798019]
[http://dx.doi.org/10.1016/j.phymed.2020.153297] [PMID: 32798019]
[8]
Yan, F.; Zhang, Q.Y.; Jiao, L.; Han, T.; Zhang, H.; Qin, L.P.; Khalid, R. Synergistic hepatoprotective effect of Schisandrae lignans with Astragalus polysaccharides on chronic liver injury in rats. Phytomedicine, 2009, 16(9), 805-813.
[http://dx.doi.org/10.1016/j.phymed.2009.02.004] [PMID: 19345075]
[http://dx.doi.org/10.1016/j.phymed.2009.02.004] [PMID: 19345075]
[9]
Du, Y.; Wan, H.; Huang, P.; Yang, J.; He, Y. A critical review of Astragalus polysaccharides: From therapeutic mechanisms to pharmaceutics. Biomed. Pharmacother., 2022, 147, 112654-112668.
[http://dx.doi.org/10.1016/j.biopha.2022.112654] [PMID: 35086031]
[http://dx.doi.org/10.1016/j.biopha.2022.112654] [PMID: 35086031]
[10]
Zhang, Y.; Zhou, Q.; Ding, X.; Wang, H.; Tan, G. HILIC-MS-based metabolomics reveal that Astragalus polysaccharide alleviates doxorubicin-induced cardiomyopathy by regulating sphingolipid and glycerophospholipid homeostasis. J. Pharm. Biomed. Anal., 2021, 203, 114177-114184.
[http://dx.doi.org/10.1016/j.jpba.2021.114177] [PMID: 34198197]
[http://dx.doi.org/10.1016/j.jpba.2021.114177] [PMID: 34198197]
[11]
Wang, L.; Shi, H.; Zhao, C.; Li, J.; Peng, J.; Shen, A.; Zhou, P.; Bian, H. Astragaloside IV protects diabetic cardiomyopathy against inflammation and apoptosis via regulating TLR4/MyD88/NF-κB signaling pathway. J. Funct. Foods, 2022, 88, 104905-104916.
[http://dx.doi.org/10.1016/j.jff.2021.104905]
[http://dx.doi.org/10.1016/j.jff.2021.104905]
[12]
Guo, K.; He, X.; Zhang, Y.; Li, X.; Yan, Z.; Pan, L.; Qin, B. Flavoniods from aerial parts of Astragalus hoantchy. Fitoterapia, 2016, 114, 34-39.
[http://dx.doi.org/10.1016/j.fitote.2016.08.009] [PMID: 27565544]
[http://dx.doi.org/10.1016/j.fitote.2016.08.009] [PMID: 27565544]
[13]
Chen, Y.; Li, L.; Xu, R.; Li, F.; Gu, L.; Liu, H.; Wang, Z.; Yang, L. Characterization of natural herbal medicines by thin-layer chromatography combined with laser ablation-assisted direct analysis in real-time mass spectrometry. J. Chromatogr. A, 2021, 1654, 462461-462469.
[http://dx.doi.org/10.1016/j.chroma.2021.462461] [PMID: 34438305]
[http://dx.doi.org/10.1016/j.chroma.2021.462461] [PMID: 34438305]
[14]
Kajino, A.; Bai, W.; Yoshimura, N.; Takayanagi, M. Identification of peach and apricot kernels for traditional Chinese medicines using near-infrared spectroscopy. Vib. Spectrosc., 2021, 113, 103202-103208.
[http://dx.doi.org/10.1016/j.vibspec.2020.103202]
[http://dx.doi.org/10.1016/j.vibspec.2020.103202]
[15]
Chang, Y.Y.; Wu, H.L.; Wang, T.; Chen, Y.; Yang, J.; Fu, H.Y.; Yang, X.L.; Li, X.F.; Zhang, G.; Yu, R.Q. Geographical origin traceability of traditional Chinese medicine Atractylodes macrocephala Koidz. by using multi-way fluorescence fingerprint and chemometric methods. Spectrochim. Acta A Mol. Biomol. Spectrosc., 2022, 269, 120737-120747.
[http://dx.doi.org/10.1016/j.saa.2021.120737] [PMID: 34959035]
[http://dx.doi.org/10.1016/j.saa.2021.120737] [PMID: 34959035]
[16]
Wen, J.; Wu, J.; Yu, H.; Fan, G.; He, Y. Correlation analysis between genetic and chemical differences of Nardostachys jatamansi from different habitats in Ganzi Tibetan Autonomous Prefecture, Sichuan Province, China. Biochem. Syst. Ecol., 2020, 92, 104133-104138.
[http://dx.doi.org/10.1016/j.bse.2020.104133]
[http://dx.doi.org/10.1016/j.bse.2020.104133]
[17]
Shi, J.; Gao, X.; Zhang, A.; Qin, X.; Du, G. Characterization of multiple chemical components of GuiLingJi by UHPLC-MS and 1H NMR analysis. J. Pharm. Anal., 2022, 12(3), 460-469.
[http://dx.doi.org/10.1016/j.jpha.2021.09.013] [PMID: 35811626]
[http://dx.doi.org/10.1016/j.jpha.2021.09.013] [PMID: 35811626]
[18]
Sun, Y.; Gong, D.; Sun, G. Quality monitoring and evaluation of Powdered Poppy Capsule Extractive by multiple wavelengths matching average fusion chromatographic fingerprint combined with electrochemical fingerprint. Microchem. J., 2021, 169, 106516-106524.
[http://dx.doi.org/10.1016/j.microc.2021.106516]
[http://dx.doi.org/10.1016/j.microc.2021.106516]
[19]
Bhattacharyya, S.; Rana, D.; Bhattacharyya, S.N. Determination of heat of formation of associated systems by calorimetry. J. Indian Chem. Soc., 1997, 74(2), 103-107.
[http://dx.doi.org/10.5281/zenodo.5875144]
[http://dx.doi.org/10.5281/zenodo.5875144]
[20]
Bhattacharyya, S.; Rana, D.; Bhattacharyya, S.N. A thermodynamic study of molecular association by gas-liquid chromatography. J. Indian Chem. Soc., 1997, 74(6), 456-463.
[http://dx.doi.org/10.5281/zenodo.5880620]
[http://dx.doi.org/10.5281/zenodo.5880620]
[21]
Bhattacharyya, S.; Rana, D.; Bhattacharyya, S.N. A thermodynamic study of molecular association by gas-liquid chromatography: Trilaurylamine alcohol systems. J. Indian Chem. Soc., 1997, 74(7), 548-551. 10.5281zenodo.5901630
[22]
Vinh, L.B.; Han, Y.K.; Park, S.Y.; Kim, Y.J.; Phong, N.V.; Kim, E.; Ahn, B.; Jung, Y.W.; Byun, Y.; Jeon, Y.H.; Lee, K.Y. Identification of triterpenoid saponin inhibitors of interleukin (IL)-33 signaling from the roots of Astragalus membranaceus. J. Funct. Foods, 2023, 101, 105418.
[http://dx.doi.org/10.1016/j.jff.2023.105418]
[http://dx.doi.org/10.1016/j.jff.2023.105418]
[23]
Yip, P.Y.; Kwan, H.S. Molecular identification of Astragalus membranaceus at the species and locality levels. J. Ethnopharmacol., 2006, 106(2), 222-229.
[http://dx.doi.org/10.1016/j.jep.2005.12.033] [PMID: 16442761]
[http://dx.doi.org/10.1016/j.jep.2005.12.033] [PMID: 16442761]
[24]
Lee, S.M.; Jeong, J.S.; Kwon, H.J.; Hong, S.P. Quantification of isoflavonoids and triterpene saponins in Astragali Radix, the root of Astragalus membranaceus, via reverse-phase high-performance liquid chromatography coupled with integrated pulsed amperometric detection. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1070, 76-81.
[http://dx.doi.org/10.1016/j.jchromb.2017.10.046] [PMID: 29102246]
[http://dx.doi.org/10.1016/j.jchromb.2017.10.046] [PMID: 29102246]
