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Current Organic Chemistry

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ISSN (Print): 1385-2728
ISSN (Online): 1875-5348

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Research Article

Stereoselective Bioreduction of Acetophenone to (R)-1-Phenylethanol by Bacillus thuringiensis

Author(s): Farah Naz Talpur*, Shafiq Ur Rahman, Ahsanullah Unar, Adnan Ibrahim, Muhammad Raza Shah, Hassan Imran Afridi, Zafar Ali and Muhammad Sohail Bashir*

Volume 27, Issue 20, 2023

Published on: 18 October, 2023

Page: [1814 - 1820] Pages: 7

DOI: 10.2174/0113852728240964231010103534

Price: $65

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Abstract

Optically pure alcohols have a pivotal synthetic role, being key intermediates for numerous pharmaceutical agents. Therefore, the synthesis of optically pure alcohols is now becoming a foremost research field in both academia and industries. Herein, Bacillus thuringiensis has been described for the first time for the bioreduction of acetophenone to 1-phenylethanol. Five incubated bacillus species and a consortium were investigated for the reduction of acetophenone. Among them, Bacillus thuringiensis (growing cells) exhibited >99% conversion efficiency of acetophenone (40 mM). The biocatalyst produced (R)-1-phenyl ethanol with excellent stereoselection (99%) at pH 7.5 after 24 h reaction intervals. To enhance the solubility of substrate and cofactor regeneration, isopropanol (10% v/v) was found to be effective among different tested cosolvents. The biocatalyst displayed excellent stereoselectivity and provided R-enantiomer with 99% enantiomeric excess.

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