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Current Analytical Chemistry

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ISSN (Print): 1573-4110
ISSN (Online): 1875-6727

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Research Article

Marine Actinobacterial Streptomyces Sp., Mediated Synthesis of Silver Nanoparticles: Characterization and Different Biological Properties Under In-vitro Condition

Author(s): Kannan Kamala, G.J. Jeevan Kumar, Dhanraj Ganapathy, Ashok K. Sundramoorthy* and Pitchiah Sivaperumal*

Volume 19, Issue 7, 2023

Published on: 05 October, 2023

Page: [550 - 560] Pages: 11

DOI: 10.2174/0115734110262574230927045451

Price: $65

Abstract

Background: Green synthesis of nanomaterials is promising as a biological source for treating different diseases without side effects.

Methods: In the present study, marine Streptomyces sp. was used to biosynthesize silver nanoparticles, which were then characterized and evaluated for various therapeutic activities and A 549 breast cancer cell line for cytotoxic evaluation.

Results: The Surface Plasmon Resonance exhibited a peak at 434 nm, and the FT-IR spectrum of St- AgNPs revealed the presence of secondary metabolites, which were used for stabilization and capping processes. The St-AgNPs showed an agglomerated spherical shape with a diameter of 10–35 nm. The elemental composition was silver (60.0%), oxygen (14.9%), sodium (14.9%), and carbon (15.0%). The St-AgNPs exhibited significant antioxidant activity in terms of DPPH 62.2 ± 2.1, H2O2 57.76 ± 2.4%, TAA 64.3±2.7, and NO 64.3 ± 2.7 at 100 μg/mL. The cytotoxic activity using A 549 Breast cancer cell line was found to be only 20 % of viable cells at 100 μg concentration. St-AgNPs revealed good antibacterial efficacy against Streptococcus mutants, Klebsiella Sp. and Staphylococcus aureus (MRSA).

Conclusion: The St-AgNPs may be a good choice for antibacterial, antioxidant and cytotoxic agents in the future with further relevant study and may be used in the field of nano biomedicine.

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