To Explore the Mechanism of Maiwei Dihuang Decoction in the Treatment
of Non-small Cell Lung Cancer based on Network Pharmacology Combined
with LC-MS

Tao      Jiang; Yang      Lu; Wanzhi      Yang; Jinhong      Xu; Mingxing      Zhu; Yong      Huang; Fang      Bao; Shengqi      Zheng; Yongxia      Li

doi:10.2174/1573409920666230823161355

Abstract

Objective: To explore the mechanism of Maiwei Dihuang decoction in the treatment of non-small cell lung cancer (NSCLC) by using network pharmacology and LC-MS technology.

Methods: The effective components in Maiwei Dihuang decoction were detected by liquid chromatography- mass spectrometry (LC-MS). Use the SuperPred database to collect the relevant targets of the active ingredients of Mai Wei Di Tang, and then collect the relevant targets of nonsmall cell lung cancer from GeneCards, DisgenNET and OMIM databases. On this basis, PPI network construction, GO enrichment analysis and KEGG pathway annotation analysis were carried out for target sites. Finally, AutoDock Vina is used for molecular docking.

Results: We further screened 16 effective Chinese herbal compounds through LC-MS combined with ADME level. On this basis, we obtained 77 core targets through protein interaction network analysis. Through GO, KEGG analysis and molecular docking results, we finally screened out the potential targets of Maiwei Dihuang Decoction for NSCLC: TP53, STAT3, MAPK3.

Conclusion: Maiwei Dihuang decoction may play a role in the treatment of NSCLC by coregulating TP53/STAT3/MAPK3 signal pathway.

« Previous Next »

Graphical Abstract

[1]
Magnuson, W.J.; Yeung, J.T.; Guillod, P.D.; Gettinger, S.N.; Yu, J.B.; Chiang, V.L. Impact of deferring radiation therapy in patients with epidermal growth factor receptor-mutant non-small cell lung cancer who develop brain metastases. Int. J. Radiat. Oncol. Biol. Phys.,  2016, 95(2), 673-679.
 [http://dx.doi.org/10.1016/j.ijrobp.2016.01.037] [PMID: 27034176]

[2]
Suwinski, R.; Giglok, M.; Galwas-Kliber, K.; Idasiak, A.; Jochymek, B.; Deja, R.; Maslyk, B.; Mrochem-Kwarciak, J.; Butkiewicz, D. Blood serum proteins as biomarkers for prediction of survival, locoregional control and distant metastasis rate in radiotherapy and radio-chemotherapy for non-small cell lung cancer. BMC Cancer,  2019, 19(1), 427.
 [http://dx.doi.org/10.1186/s12885-019-5617-1] [PMID: 31068179]

[3]
Fan, T.W.M.; Zhang, X.; Wang, C.; Yang, Y.; Kang, W.Y.; Arnold, S.; Higashi, R.M.; Liu, J.; Lane, A.N. Exosomal lipids for classifying early and late stage non-small cell lung cancer. Anal. Chim. Acta,  2018, 1037, 256-264.
 [http://dx.doi.org/10.1016/j.aca.2018.02.051] [PMID: 30292300]

[4]
Greenberg, A.K.; Lee, M.S. Biomarkers for lung cancer: Clinical uses. Curr. Opin. Pulm. Med.,  2007, 13(4), 249-255.
 [http://dx.doi.org/10.1097/MCP.0b013e32819f8f06] [PMID: 17534168]

[5]
Hayat, M.J.; Howlader, N.; Reichman, M.E.; Edwards, B.K. Cancer statistics, trends, and multiple primary cancer analyses from the surveillance, epidemiology and end results (SEER) program. Oncologist,  2007, 12(1), 20-37.
 [http://dx.doi.org/10.1634/theoncologist.12-1-20] [PMID: 17227898]

[6]
Griesinger, F.; Korol, E.E.; Kayaniyil, S.; Varol, N.; Ebner, T.; Goring, S.M. Efficacy and safety of first-line carboplatin-versus cisplatin-based chemotherapy for non-small cell lung cancer: A meta-analysis. Lung Cancer,  2019, 135, 196-204.
 [http://dx.doi.org/10.1016/j.lungcan.2019.07.010] [PMID: 31446995]

[7]
de Castria, T.B.; da Silva, E.M.K.; Gois, A.F.T.; Riera, R. Cisplatin versus carboplatin in combination with third-generation drugs for advanced non-small cell lung cancer. Cochrane Libr.,  2013, (8), CD009256.
 [http://dx.doi.org/10.1002/14651858.CD009256.pub2] [PMID: 23949842]

[8]
Wang, S.; Wu, X.; Tan, M.; Gong, J.; Tan, W.; Bian, B.; Chen, M.; Wang, Y. Fighting fire with fire: Poisonous Chinese herbal medicine for cancer therapy. J. Ethnopharmacol.,  2012, 140(1), 33-45.
 [http://dx.doi.org/10.1016/j.jep.2011.12.041] [PMID: 22265747]

[9]
Zhong, Z.; Yu, H.; Wang, S.; Wang, Y.; Cui, L. Anti-cancer effects of Rhizoma Curcumae against doxorubicin-resistant breast cancer cells. Chin. Med.,  2018, 13(1), 44.
 [http://dx.doi.org/10.1186/s13020-018-0203-z] [PMID: 30181769]

[10]
Sang, W.; Zhong, Z.; Linghu, K.; Xiong, W.; Tse, A.K.W.; Cheang, W.S.; Yu, H.; Wang, Y. Siegesbeckia pubescens Makino inhibits Pam3CSK4-induced inflammation in RAW 264.7 macrophages through suppressing TLR1/TLR2-mediated NF-κB activation. Chin. Med.,  2018, 13(1), 37.
 [http://dx.doi.org/10.1186/s13020-018-0193-x] [PMID: 30002726]

[11]
Zhong, Z.; Zhang, Q.; Tao, H.; Sang, W.; Cui, L.; Qiang, W.; Cheang, W.S.; Hu, Y.; Yu, H.; Wang, Y. Anti-inflammatory activities of Sigesbeckia glabrescens Makino: Combined in vitro and in silico investigations. Chin. Med.,  2019, 14(1), 35.
 [http://dx.doi.org/10.1186/s13020-019-0260-y] [PMID: 31572487]

[12]
Liu, Y.; Wang, X.; Zeng, S.; Zhang, X.; Zhao, J.; Zhang, X.; Chen, X.; Yang, W.; Yang, Y.; Dong, Z.; Zhu, J.; Xu, X.; Tian, F. The natural polyphenol curcumin induces apoptosis by suppressing STAT3 signaling in esophageal squamous cell carcinoma. J. Exp. Clin. Cancer Res.,  2018, 37(1), 303.
 [http://dx.doi.org/10.1186/s13046-018-0959-0] [PMID: 30518397]

[13]
Liu, D.; You, M.; Xu, Y.; Li, F.; Zhang, D.; Li, X.; Hou, Y. Inhibition of curcumin on myeloid-derived suppressor cells is requisite for controlling lung cancer. Int. Immunopharmacol.,  2016, 39, 265-272.
 [http://dx.doi.org/10.1016/j.intimp.2016.07.035] [PMID: 27497194]

[14]
Samarghandian, S.; Azimi-Nezhad, M.; Farkhondeh, T.; Samini, F. Anti-oxidative effects of curcumin on immobilization-induced oxidative stress in rat brain, liver and kidney. Biomed. Pharmacother.,  2017, 87, 223-229.
 [http://dx.doi.org/10.1016/j.biopha.2016.12.105] [PMID: 28061405]

[15]
Chen, P.; Huang, H.P.; Wang, Y.; Jin, J.; Long, W.G.; Chen, K.; Zhao, X.H.; Chen, C.G.; Li, J. Curcumin overcome primary gefitinib resistance in non-small-cell lung cancer cells through inducing autophagy-related cell death. J. Exp. Clin. Cancer Res.,  2019, 38(1), 254.
 [http://dx.doi.org/10.1186/s13046-019-1234-8] [PMID: 31196210]

[16]
Miller, K.D.; Nogueira, L.; Devasia, T.; Mariotto, A.B.; Yabroff, K.R.; Jemal, A.; Kramer, J.; Siegel, R.L. Cancer treatment and survivorship statistics, 2022. CA Cancer J. Clin.,  2022, 72(5), 409-436.
 [http://dx.doi.org/10.3322/caac.21731] [PMID: 35736631]

[17]
Devlin, J.R.; Verschuren, E.W. More than a tumor suppressor: E-Cadherin loss drives lung cancer metastasis. Am. J. Respir. Cell Mol. Biol.,  2018, 59(2), 141-142.
 [http://dx.doi.org/10.1165/rcmb.2018-0063ED] [PMID: 29688752]

[18]
Rosenblum, A.M.; Chasen, M. Comprehensive metastatic lung cancer care must include palliative care. Curr. Oncol.,  2018, 25(3), 192-193.
 [http://dx.doi.org/10.3747/co.25.4104] [PMID: 29962835]

[19]
Altorki, N.K.; Markowitz, G.J.; Gao, D.; Port, J.L.; Saxena, A.; Stiles, B.; McGraw, T.; Mittal, V. The lung microenvironment: An important regulator of tumour growth and metastasis. Nat. Rev. Cancer,  2019, 19(1), 9-31.
 [http://dx.doi.org/10.1038/s41568-018-0081-9] [PMID: 30532012]

[20]
Tandberg, D.J.; Tong, B.C.; Ackerson, B.G.; Kelsey, C.R. Surgery versus stereotactic body radiation therapy for stage I non-small cell lung cancer: A comprehensive review. Cancer,  2018, 124(4), 667-678.
 [http://dx.doi.org/10.1002/cncr.31196] [PMID: 29266226]

[21]
Lalla, R.V.; Brennan, M.T.; Gordon, S.M.; Sonis, S.T.; Rosenthal, D.I.; Keefe, D.M. Oral mucositis due to high-dose chemotherapy and/or head and neck radiation therapy. J. Natl. Cancer Inst. Monogr.,  2019, 2019(53), lgz011.
 [PMID: 31425601]

[22]
Li, T.M.; Yu, Y.H.; Tsai, F.J.; Cheng, C.F.; Wu, Y.C.; Ho, T.J.; Liu, X.; Tsang, H.; Lin, T.H.; Liao, C.C.; Huang, S.M.; Li, J.P.; Lin, J.C.; Lin, C.C.; Liang, W.M.; Lin, Y.J. Characteristics of Chinese herbal medicine usage and its effect on survival of lung cancer patients in Taiwan. J. Ethnopharmacol.,  2018, 213, 92-100.
 [http://dx.doi.org/10.1016/j.jep.2017.10.031] [PMID: 29100936]

[23]
Liao, Y.H.; Li, C.I.; Lin, C.C.; Lin, J.G.; Chiang, J.H.; Li, T.C. Traditional Chinese medicine as adjunctive therapy improves the long-term survival of lung cancer patients. J. Cancer Res. Clin. Oncol.,  2017, 143(12), 2425-2435.
 [http://dx.doi.org/10.1007/s00432-017-2491-6] [PMID: 28803328]

[24]
Li, Z.; Feiyue, Z.; Gaofeng, L. Traditional Chinese medicine and lung cancer-From theory to practice. Biomed. Pharmacother.,  2021, 137, 111381.
 [http://dx.doi.org/10.1016/j.biopha.2021.111381] [PMID: 33601147]

[25]
Wang, Q.; Jiao, L.; Wang, S.; Chen, P.; Bi, L.; Zhou, D.; Yao, J.; Li, J.; Wang, L.; Chen, Z.; Jia, Y.; Zhang, Z.; Shen, W.; Zhu, W.; Xu, J.; Gao, Y.; Xu, L.; Gong, Y. Adjuvant chemotherapy with chinese herbal medicine formulas versus placebo in patients with lung adenocarcinoma after radical surgery: A multicenter, randomized, double-blind, placebo-controlled trial. Biol. Proced. Online,  2020, 22(1), 5.
 [http://dx.doi.org/10.1186/s12575-020-00117-5] [PMID: 32140080]

[26]
Li, H.; Ji, Y.; Zhang, S.; Gao, Z.; Hu, C.; Jiang, R.; Chen, M.; Li, G.; Zhang, X. Kangai injection combined with platinum-based chemotherapy for the treatment of stage III/IV non-small cell lung cancer: A meta-analysis and systematic review of 35 randomized controlled trials. J. Cancer,  2019, 10(21), 5283-5298.
 [http://dx.doi.org/10.7150/jca.31928] [PMID: 31602279]

[27]
Jiang, Y.; Liu, L.S.; Shen, L.P.; Han, Z.F.; Jian, H.; Liu, J.X.; Xu, L.; Li, H.G.; Tian, J.H.; Mao, Z.J. Traditional chinese medicine treatment as maintenance therapy in advanced non-small-cell lung cancer: A randomized controlled trial. Complement. Ther. Med.,  2016, 24, 55-62.
 [http://dx.doi.org/10.1016/j.ctim.2015.12.006] [PMID: 26860802]

[28]
Mogi, A.; Kuwano, H. TP53 mutations in nonsmall cell lung cancer. J. Biomed. Biotechnol.,  2011, 2011, 1-9.
 [http://dx.doi.org/10.1155/2011/583929] [PMID: 21331359]

[29]
Baumann, M.; Zips, D.; Appold, S. Radiotherapy of lung cancer: Technology meets biology meets multidisciplinarity. Radiother. Oncol.,  2009, 91(3), 279-281.
 [http://dx.doi.org/10.1016/j.radonc.2009.05.001] [PMID: 19464524]

[30]
Viktorsson, K.; De Petris, L.; Lewensohn, R. The role of p53 in treatment responses of lung cancer. Biochem. Biophys. Res. Commun.,  2005, 331(3), 868-880.
 [http://dx.doi.org/10.1016/j.bbrc.2005.03.192] [PMID: 15865943]

[31]
Steels, E.; Paesmans, M.; Berghmans, T.; Branle, F.; Lemaitre, F.; Mascaux, C.; Meert, A.P.; Vallot, F.; Lafitte, J.J.; Sculier, J.P. Role of p53 as a prognostic factor for survival in lung cancer: A systematic review of the literature with a meta-analysis. Eur. Respir. J.,  2001, 18(4), 705-719.
 [http://dx.doi.org/10.1183/09031936.01.00062201] [PMID: 11716177]

[32]
Zheng, Q.; Dong, H.; Mo, J.; Zhang, Y.; Huang, J.; Ouyang, S.; Shi, S.; Zhu, K.; Qu, X.; Hu, W.; Liu, P.; Wang, Y.; Zhang, X. A novel STAT3 inhibitor W2014-S regresses human non-small cell lung cancer xenografts and sensitizes EGFR-TKI acquired resistance. Theranostics,  2021, 11(2), 824-840.
 [http://dx.doi.org/10.7150/thno.49600] [PMID: 33391507]

[33]
Harada, D.; Takigawa, N.; Kiura, K. The role of STAT3 in non-small cell lung cancer. Cancers,  2014, 6(2), 708-722.
 [http://dx.doi.org/10.3390/cancers6020708] [PMID: 24675568]

[34]
Yue, P.; Turkson, J. Targeting STAT3 in cancer: How successful are we? Expert Opin. Investig. Drugs,  2009, 18(1), 45-56.
 [http://dx.doi.org/10.1517/13543780802565791] [PMID: 19053881]

[35]
Huang, Q.; Zhong, Y.; Dong, H.; Zheng, Q.; Shi, S.; Zhu, K.; Qu, X.; Hu, W.; Zhang, X.; Wang, Y. Revisiting signal transducer and activator of transcription 3 (STAT3) as an anticancer target and its inhibitor discovery: Where are we and where should we go? Eur. J. Med. Chem.,  2020, 187, 111922.
 [http://dx.doi.org/10.1016/j.ejmech.2019.111922] [PMID: 31810784]

[36]
Furtek, S.L.; Backos, D.S.; Matheson, C.J.; Reigan, P. Strategies and approaches of targeting STAT3 for cancer treatment. ACS Chem. Biol.,  2016, 11(2), 308-318.
 [http://dx.doi.org/10.1021/acschembio.5b00945] [PMID: 26730496]

[37]
Wingelhofer, B.; Neubauer, H.A.; Valent, P.; Han, X.; Constantinescu, S.N.; Gunning, P.T.; Müller, M.; Moriggl, R. Implications of STAT3 and STAT5 signaling on gene regulation and chromatin remodeling in hematopoietic cancer. Leukemia,  2018, 32(8), 1713-1726.
 [http://dx.doi.org/10.1038/s41375-018-0117-x] [PMID: 29728695]

[38]
Fan, J.; Ren, D.; Wang, J.; Liu, X.; Zhang, H.; Wu, M.; Yang, G. Bruceine D induces lung cancer cell apoptosis and autophagy via the ROS/MAPK signaling pathway in vitro and in vivo. Cell Death Dis.,  2020, 11(2), 126.
 [http://dx.doi.org/10.1038/s41419-020-2317-3] [PMID: 32071301]

Rights & Permissions Print Cite

Journal Information

For Authors

For Editors

For Reviewers

Explore Articles

Open Access

Open Access Articles

For Visitors

DOI https://dx.doi.org/10.2174/1573409920666230823161355	Print ISSN 1573-4099
Publisher Name Bentham Science Publisher	Online ISSN 1875-6697

Current Computer-Aided Drug Design

To Explore the Mechanism of Maiwei Dihuang Decoction in the Treatment of Non-small Cell Lung Cancer based on Network Pharmacology Combined with LC-MS

Abstract Play Pause

Graphical Abstract

Related Journals

Related Books

Abstract