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Recent Innovations in Chemical Engineering

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ISSN (Print): 2405-5204
ISSN (Online): 2405-5212

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Research Article

Evaluation of Dose-dependent Interactions of Antibiotics using RBC Membrane Model Systems

Author(s): Sriharini S., Sugantha Bharathy S. and Stalin Selvaraj*

Volume 16, Issue 3, 2023

Published on: 04 September, 2023

Page: [167 - 173] Pages: 7

DOI: 10.2174/2405520416666230815124135

Price: $65

Abstract

Background: The present study aims to understand the membrane interactions of three different antibiotics widely used for non-specific viral diseases.

Methods: The dose-dependent membrane interactions of the selected antibiotics were elucidated using octanol: water partition coefficient and hemolysis assay.

Results: Our octanol: water partition coefficient experiments indicated that among the three antibiotics (Azithromycin, Erythromycin, and Amoxicillin), azithromycin and erythromycin were more hydrophobic compared to amoxicillin. This finding also correlated with hypotonic hemolysis assay experiments where the membrane stabilization effects of both azithromycin and erythromycin were reduced in a dose-dependent manner compared to amoxicillin.

Conclusion: This may be attributed to the hydrophobic nature of azithromycin, and erythromycin penetrates deeper into the RBC membrane to the extent of the hydrophobic core compared to amoxicillin. The result is also concurrent with the observations from the hemolysis assay in the isotonic conditions. To conclude, among the three antibiotics, azithromycin, erythromycin localized on the hydrophobic core of the lipid membrane, and amoxicillin localized on the outer surface and slight penetration to the outer leaflet of the membrane.

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[1]
Ahmed HHB, Al-Hadiya BMH, Abd-Elgalil AA. Azithromycin. Profiles Drug Subst Excip Relat Methodol 2014; 39: 1-40.
[2]
Firth A, Prathapan P. Azithromycin: The first broad-spectrum therapeutic. Eur J Med Chem 2020; 207: 112739.
[http://dx.doi.org/10.1016/j.ejmech.2020.112739] [PMID: 32871342]
[3]
Oliver ME, Hinks TSC. Azithromycin in viral infections. Rev Med Virol 2021; 31(2): e2163.
[http://dx.doi.org/10.1002/rmv.2163] [PMID: 32969125]
[4]
Brittain DC. Erythromycin. Med Clin North Am 1987; 71(6): 1147-54.
[http://dx.doi.org/10.1016/S0025-7125(16)30802-1] [PMID: 3320616]
[5]
Latare PA, Setness PA. Using erythromycin. Postgrad Med 1989; 86(1): 55-63.
[http://dx.doi.org/10.1080/00325481.1989.11704323] [PMID: 2662161]
[6]
Alvarez-Elcoro S, Enzler MJ. The macrolides: Erythromycin, clarithromycin, and azithromycin. Mayo Clin Proc 1999; 74(6): 613-34.
[http://dx.doi.org/10.4065/74.6.613] [PMID: 10377939]
[7]
de Marco BA, Natori JSH, Fanelli S, Tótoli EG, Salgado HRN. Characteristics, properties and analytical methods of amoxicillin: A review with green approach. Crit Rev Anal Chem 2017; 47(3): 267-77.
[http://dx.doi.org/10.1080/10408347.2017.1281097] [PMID: 28080135]
[8]
Natarajan S, Anbarasi C, Sathiyarajeswaran P, et al. Kabasura Kudineer (KSK), a poly-herbal Siddha medicine, reduced SARS-CoV-2 viral load in asymptomatic COVID-19 individuals as compared to vitamin C and zinc supplementation: findings from a prospective, exploratory, open-labeled, comparative, randomized controlled trial, Tamil Nadu, India. Trials 2021; 22(1): 623.
[http://dx.doi.org/10.1186/s13063-021-05583-0] [PMID: 34526104]
[9]
Bala D. Attitudes, beliefs, and self-use of Kabasura Kudineer among urban and rural population in Tamil Nadu, India: A comparative cross-sectional study. J Family Med Prim Care 2021; 10(1): 158-66.
[http://dx.doi.org/10.4103/jfmpc.jfmpc_1634_20] [PMID: 34017720]
[10]
Parthasarathy R, Mathew M, Koshy P, Babu M, Abraham G. Traditional medicines prescribed for prevention of COVID -19: Use with caution. Nephrology 2021; 26(12): 961-4.
[http://dx.doi.org/10.1111/nep.13947] [PMID: 34322937]
[11]
Lúcio M, Lima JLFC, Reis S. Drug-membrane interactions: Significance for medicinal chemistry. Curr Med Chem 2010; 17(17): 1795-809.
[http://dx.doi.org/10.2174/092986710791111233] [PMID: 20345343]
[12]
Pinheiro M. Special issue on drug–membrane interactions. Membranes 2021; 11(10): 764.
[http://dx.doi.org/10.3390/membranes11100764] [PMID: 34677530]
[13]
Nedyalkova MA, Madurga S, Tobiszewski M, Simeonov V. Calculating the partition coefficients of organic solvents in octanol/water and octanol/air. J Chem Inf Model 2019; 59(5): 2257-63.
[http://dx.doi.org/10.1021/acs.jcim.9b00212] [PMID: 31042037]
[14]
Bunea AI, Harloff-Helleberg S, Taboryski R, Nielsen HM. Membrane interactions in drug delivery: Model cell membranes and orthogonal techniques. Adv Colloid Interface Sci 2020; 281: 102177.
[http://dx.doi.org/10.1016/j.cis.2020.102177] [PMID: 32417568]
[15]
Selvaraj S, Krishnaswamy S, Devashya V, Sethuraman S, Krishnan UM. Investigations on membrane perturbation by chrysin and its copper complex using self-assembled lipid bilayers. Langmuir 2011; 27(21): 13374-82.
[http://dx.doi.org/10.1021/la2029356] [PMID: 21923196]
[16]
Selvaraj S, Krishnaswamy S, Devashya V, Sethuraman S, Krishnan UM. Membrane fluidization & eryptotic properties of hesperidin–copper complex. RSC Advances 2012; 2(29): 11138-46.
[http://dx.doi.org/10.1039/c2ra20620j]
[17]
Blaskó K, Shagina LV, Györgyi S, Lev AA. The mode of action of some antibiotics on red blood cell membranes. Gen Physiol Biophys 1986; 5(6): 625-36.
[PMID: 2435616]
[18]
Suwalsky M, Villena F, Aguilar F, Sotomayor CP. Interaction of penicillin G with the human erythrocyte membrane and models. Z Naturforsch C J Biosci 1996; 51(3-4): 243-8.
[http://dx.doi.org/10.1515/znc-1996-3-416] [PMID: 8639231]
[19]
Srivastava A, Rengaraju M, Srivastava S, Narayan V, Gupta V, Upadhayay R. A double blinded placebo controlled comparative clinical trial to evaluate the effectiveness of Siddha medicines, Kaba Sura Kudineer (KSK) & Nilavembu Kudineer (NVK) along with standard Allopathy treatment in the management of symptomatic COVID 19 patients - a structured summary of a study protocol for a randomized controlled trial. Trials 2021; 22(1): 130.
[http://dx.doi.org/10.1186/s13063-021-05041-x] [PMID: 33573696]

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