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Recent Advances in Anti-Infective Drug Discovery

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ISSN (Print): 2772-4344
ISSN (Online): 2772-4352

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Research Article

Investigation of Antifungal Action of Fractions C17H31NO15 Isolated from Artemisia herba-alba extract versus Isolated Aspergillus niger from Zee maize

Author(s): Basma Hamdy Amin*, Nahed Mohammed Ayyat, Reyad Mohamed El-Sharkawy and Asmaa Mohamed Hafez

Volume 19, Issue 2, 2024

Published on: 18 September, 2023

Page: [159 - 172] Pages: 14

DOI: 10.2174/2772434418666230627141639

Price: $65

Abstract

Background: Plants are harmed by parasitic organisms, and toxic poisons are created. Phytopathogenic fungi create toxins that can severely harm plants' basic physiological functioning.

Objective: Investigation of antifungal impact of various fractions of methanol extract of Artemisia herba-alba to Aspergillus niger as a plant pathogen.

Methods: Artemisia herba-alba extract was purified using column chromatography, giving various antifungal fractions tested versus A. niger.

Results: The 6th fraction give the highest inhibition zone with a diameter of 5.4 cm and MIC 125.02 ± 4.9 μg/ml, which was identified using Mass spectroscopy, 1HNMR, Elemental analysis as well as IR testing, revealing the chemical formula of the purified fraction. Ultrastructure alteration of treated A. niger was examined versus control using the transmission electron microscope. Purified fraction has tested versus normal cell line with minimal cytotoxicity.

Conclusion: These results revealed the possibility of using Artemisia herba-alba methanol extract as a promising antifungal versus phytopathogenic fungi, especially A. niger after more verification of results.

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