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Current Neurovascular Research

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ISSN (Print): 1567-2026
ISSN (Online): 1875-5739

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Research Article

Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia

Author(s): Tongjun Ma, Chenyu Li, Zeyin Nie, Huachun Miao and Feng Wu*

Volume 20, Issue 2, 2023

Published on: 23 June, 2023

Page: [237 - 243] Pages: 7

DOI: 10.2174/1567202620666230612110156

Price: $65

Abstract

Background: Previous studies have shown that the neurological damage caused by middle cerebral artery occlusion (MCAO) is not only limited to local infarction but can also cause secondary damage in distant sites, such as the hypothalamus. 5-hydroxytryptamine (5-HT)/ 5-HT transporter (5-HTT) and 5-HT receptor 2A (5-HT2A) are important in the treatment of cerebrovascular diseases.

Objective: This study aimed to study the effect of electroacupuncture (EA) on the expression of 5- HT, 5-HTT, and 5-HT2A in the hypothalamus of rats with ischemic brain injury and to explore the protective effect and potential mechanism of EA on the secondary injury of cerebral ischemia.

Methods: Sprague-Dawley (SD) rats were randomly divided into three groups: sham group, model group, and EA group. The permanent middle cerebral artery occlusion (pMCAO) method was used to induce ischemic stroke in rats. In the EA group, the Baihui (GV20) and Zusanli (ST36) points were selected for treatment, which was administered once per day for two consecutive weeks. The neuroprotective effect of EA was evaluated by nerve defect function scores and Nissl staining. The content of 5-HT in hypothalamus was detected by enzyme linked immunosorbent assay (ELISA), and the expression of 5-HTT and 5-HT2A were detected by Western blot.

Results: Compared with that in the sham group, the nerve defect function score in the model group rats was significantly increased, the hypothalamus tissue showed obvious nerve damage, the levels of 5-HT and the expression of 5-HTT were significantly reduced, and the expression of 5-HT2A was significantly increased. After 2 weeks of EA treatment, the nerve defect function scores of pMCAO rats were significantly reduced, the hypothalamic nerve injury was significantly reduced, the levels of 5-HT and the expression of 5-HTT were significantly increased, and the expression of 5-HT2A was significantly decreased.

Conclusion: EA has a certain therapeutic effect on hypothalamic injury secondary to permanent cerebral ischemia, and its potential mechanism may be closely related to the upregulation of 5-HT and 5-HTT expression and the downregulation of 5-HT2A expression.

« Previous Next »
[1]
El Amki M, Clavier T, Perzo N, Bernard R, Guichet PO, Castel H. Hypothalamic, thalamic and hippocampal lesions in the mouse MCAO model: Potential involvement of deep cerebral arteries? J Neurosci Methods 2015; 254: 80-5.
[http://dx.doi.org/10.1016/j.jneumeth.2015.07.008] [PMID: 26213218]
[2]
Alrafiah AR. Secondary cerebellar cortex injury in albino male rats after MCAO: A histological and biochemical study. Biomedicines 2021; 9(9): 1267.
[http://dx.doi.org/10.3390/biomedicines9091267] [PMID: 34572453]
[3]
Yu B, Ruan M, Liang T, et al. Tetramethylpyrazine phosphate and borneol combination therapy synergistically attenuated ischemia-reperfusion injury of the hypothalamus and striatum via regulation of apoptosis and autophagy in a rat model. Am J Transl Res 2017; 9(11): 4807-20.
[PMID: 29218081]
[4]
Chen SH, Lin MT, Chang CP. Ischemic and oxidative damage to the hypothalamus may be responsible for heat stroke. Curr Neuropharmacol 2013; 11(2): 129-40.
[http://dx.doi.org/10.2174/1570159X11311020001] [PMID: 23997749]
[5]
Ghosh A, Sarkar S, Mandal AK, Das N. Neuroprotective role of nanoencapsulated quercetin in combating ischemia-reperfusion induced neuronal damage in young and aged rats. PLoS One 2013; 8(4): e57735.
[http://dx.doi.org/10.1371/journal.pone.0057735] [PMID: 23620721]
[6]
Yi C, Wang J, Wang Y, Wu X. Neuroprotection by 2,3,5,4ʹ-tetrahydroxystilbene-2-O-β-D-glucoside extracts from Polygonum multiflorum against cerebral ischemia/reperfusion injury through the 5-hydroxytryptamine/5-hydroxytryptamine receptor pathway. Neuropsychiatr Dis Treat 2019; 15: 1429-38.
[http://dx.doi.org/10.2147/NDT.S179845] [PMID: 31213817]
[7]
Lan X, Zhang M, Yang W, et al. Effect of treadmill exercise on 5-HT, 5-HT1A receptor and brain derived neurophic factor in rats after permanent middle cerebral artery occlusion. Neurol Sci 2014; 35(5): 761-6.
[http://dx.doi.org/10.1007/s10072-013-1599-y] [PMID: 24326625]
[8]
Tang H, Gamdzyk M, Huang L, et al. Delayed recanalization after MCAO ameliorates ischemic stroke by inhibiting apoptosis via HGF/c-Met/STAT3/Bcl-2 pathway in rats. Exp Neurol 2020; 330: 113359.
[http://dx.doi.org/10.1016/j.expneurol.2020.113359] [PMID: 32428505]
[9]
Damsbo AG, Kraglund KL, Buttenschøn HN, Johnsen SP, Andersen G, Mortensen JK. Serotonergic regulation and cognition after stroke: The role of antidepressant treatment and genetic variation. Cerebrovasc Dis 2019; 47(1-2): 72-9.
[http://dx.doi.org/10.1159/000498911] [PMID: 30844812]
[10]
Wang C, Wu C, Yan Z, Cheng X. Ameliorative effect of Xiaoyao-jieyu-san on post-stroke depression and its potential mechanisms. J Nat Med 2019; 73(1): 76-84.
[http://dx.doi.org/10.1007/s11418-018-1243-5] [PMID: 30194657]
[11]
Yang Y, Deng P, Si Y, Xu H, Zhang J, Sun H. Acupuncture at GV20 and ST36 improves the recovery of behavioral activity in rats subjected to Cerebral Ischemia/Reperfusion Injury. Front Behav Neurosci 2022; 16: 909512.
[http://dx.doi.org/10.3389/fnbeh.2022.909512] [PMID: 35775011]
[12]
Xu H, Zhang Y, Sun H, Chen S, Si Y. Electroacupuncture at GV20 and ST36 Exerts neuroprotective effects via the EPO-Mediated JAK2/STAT3 pathway in cerebral ischemic rats. Evid Based Complement Alternat Med 2017; 2017: 1-11.
[http://dx.doi.org/10.1155/2017/6027421] [PMID: 28848617]
[13]
Zhang Y, Xu H, Sun H, Chen S, Wang F. Electroacupuncture treatment improves neurological function associated with regulation of tight junction proteins in rats with cerebral ischemia reperfusion injury. Evid Based Complement Alternat Med 2014; 2014: 1-10.
[http://dx.doi.org/10.1155/2014/989340] [PMID: 25009574]
[14]
Xu H, Zhang Y, Sun H, Chen S, Wang F. Effects of acupuncture at GV20 and ST36 on the expression of matrix metalloproteinase 2, aquaporin 4, and aquaporin 9 in rats subjected to cerebral ischemia/reperfusion injury. PLoS One 2014; 9(5): e97488.
[http://dx.doi.org/10.1371/journal.pone.0097488] [PMID: 24828425]
[15]
Cui J, Song W, Jin Y, et al. Research progress on the mechanism of the acupuncture regulating neuro-endocrine-immune network system. Vet Sci 2021; 8(8): 149.
[http://dx.doi.org/10.3390/vetsci8080149] [PMID: 34437474]
[16]
Deng P, Wang L, Zhang Q, et al. Therapeutic potential of a combination of electroacupuncture and human iPSC-derived small extracellular vesicles for ischemic stroke. Cells 2022; 11(5): 820.
[http://dx.doi.org/10.3390/cells11050820] [PMID: 35269441]
[17]
Tang L, You F, Hu X, Li YF. Electroacupuncture improves insomnia by down-regulating peripheral benzodiazepine receptor expression in hippocampus, and up-regulating 5-HT, 5-HIAA, TNF-α and IL-1β contents in hypothalamus in insomnia rats. Zhen Ci Yan Jiu 2019; 44(8): 560.
[18]
Mazza M, Demetrios Kotzalidis G, Marano G, et al. Lorcaserin: Worthy of further insights? Results from recent research. CNS Neurol Disord Drug Targets 2023; 22.
[http://dx.doi.org/10.2174/1871527322666230330124137] [PMID: 37005521]
[19]
Zhang X, Wang CB, Duan LH, et al. Correlation research of serum substance P, CCK-8, and 5-HT values with depression levels in stroke survivors. Eur Rev Med Pharmacol Sci 2023; 27(4): 1248-54.
[http://dx.doi.org/10.26355/eurrev_202302_31357] [PMID: 36876663]
[20]
Zhang W, Han L, Wen Y, Su L, Li Y, Luo X. Electroacupuncture reverses endothelial cell death and promotes angiogenesis through the VEGF/Notch signaling pathway after focal cerebral ischemia‐reperfusion injury. Brain Behav 2023; 13(3): e2912.
[http://dx.doi.org/10.1002/brb3.2912] [PMID: 36786352]
[21]
Lan W, Li Z, Li YH, Song SZ. Acupuncture combined with exercise training at different time points on nerve repair of cerebral ischemia-reperfusion injury in rats and its effects on the expressions of Nestin, bFGF and EGF. Eur Rev Med Pharmacol Sci 2023; 27(1): 38-45.
[http://dx.doi.org/10.26355/eurrev_202301_30851] [PMID: 36651839]
[22]
Zhang Q, Deng P, Chen S, et al. Electroacupuncture and human iPSC-derived small extracellular vesicles regulate the gut microbiota in ischemic stroke via the brain-gut axis. Front Immunol 2023; 14: 1107559.
[http://dx.doi.org/10.3389/fimmu.2023.1107559] [PMID: 36742316]

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