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Current Drug Therapy

Editor-in-Chief

ISSN (Print): 1574-8855
ISSN (Online): 2212-3903

Research Article

Characterization and Extraction of Bioactive Compounds in Ethanolic Extract of Moringa concanensis, Nimmo Leaf, Using Microwave-assisted and Soxhlet Techniques

Author(s): Amerendra Singh*, Santosh Kumar Singh, Prabhat Kumar Upadhyay, Jai Narayan Mishra and Vishal Kumar Vishwakarma

Volume 19, Issue 2, 2024

Published on: 13 June, 2023

Page: [233 - 238] Pages: 6

DOI: 10.2174/1574885518666230522122556

Price: $65

Abstract

Background: The extraction of Moringa concanensis (Nimmo leaf) was done in ethanol using Soxhlet and microwave-assisted extraction (MAE) techniques to obtain bioactive compounds.

Methods: The standard techniques were used to carry out phytochemical analysis of ethanolic extract. The analysis confirmed the presence of flavonoids, phenols, steroids, alkaloids, terpenoids, glycosides, tannins, and saponins, and also the absence of anthraquinones. Moreover, the extract was evaluated for antioxidant activities using DPPH and ABTS assays. The IC50 value of the extract was also determined.

Results: A clear correlation between the total phenolic content, total flavonoid content, and antioxidant activity of the leaf extracts was obtained.

Conclusion: This study suggests that a high amount of phenolic compounds could be obtained from Moringa concanensis (Nimmo leaf) by using the microwave-assisted extraction technique, which may be responsible for its antioxidant activity.

Graphical Abstract

[1]
Danhier F, Feron O, Préat V. To exploit the tumor microenvironment: Passive and active tumor targeting of nanocarriers for anti-cancer drug delivery. J Control Release 2010; 148(2): 135-46.
[http://dx.doi.org/10.1016/j.jconrel.2010.08.027] [PMID: 20797419]
[2]
Wang H, Oo Khor T, Shu L, et al. Plants vs. cancer: A review on natural phytochemicals in preventing and treating cancers and their druggability. Anticancer Agents Med Chem 2012; 12(10): 1281-305.
[http://dx.doi.org/10.2174/187152012803833026] [PMID: 22583408]
[3]
Dubey NK, Kumar R, Tripathi P. Global promotion of herbal medicine: India’s opportunity. Curr Sci 2004; 86(1): 37-41.
[4]
Sekhar C, Venkatesan N, Murugananthi D, Vidhyavathi A. Status of value addition and export of moringa produce in Tamil Nadu-a case study. Int J Hort 2018.
[http://dx.doi.org/10.5376/ijh.2018.08.0003]
[5]
Khalafalla MM, Abdellatef E, Dafalla HM, et al. Active principle from Moringa oleifera Lam leaves effective against two leukemias and a hepatocarcinoma. Afr J Biotechnol 2010; 9(49): 8467-71.
[6]
Olson M. Flora of north america editorial committee. Moringaceae Martinov. Drumstick Tree Family. Flora of North America 1993; pp. 167-9.
[7]
Fahey JW. Moringa oleifera: A review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J 2005; 1(5): 1-5.
[8]
James A, Zikankuba V. Moringa oleifera a potential tree for nutrition security in sub-Sahara Africa. Am J Res Commun 2017; 5: 1-4.
[9]
Kirtikar KR, Basu BD. Indian medicinal plants. Allahabad, India 1935.
[10]
Kasolo JN, Bimenya GS, Ojok L, Ochieng J, Ogwal-Okeng JW. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants Res 2010; 4(9): 753-7.
[11]
Titi MK, Harijono ET, Endang SW. Effect lactagogue moringa leaves (Moringa oleifera Lam) powder in rats white female wistar. J Basic Appl Sci Res 2013; 3(4): 430-4.
[12]
Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: A review. Front Pharmacol 2012; 3: 24.
[http://dx.doi.org/10.3389/fphar.2012.00024] [PMID: 22403543]
[13]
Alara OR, Abdurahman NH, Ukaegbu CI. Soxhlet extraction of phenolic compounds from Vernonia cinerea leaves and its antioxidant activity. J Appl Res Med Aromat Plants 2018; 11: 12-7.
[http://dx.doi.org/10.1016/j.jarmap.2018.07.003]
[14]
Raut P, Bhosle D, Janghel A. Emerging microwave assisted extraction (MAE) techniques as an innovative green technologies for the effective extraction of the active phytopharmaceuticals. Res J Pharm Technol 2015; 8(6): 655-66.
[15]
Lee NY, Yunus MA, Idham Z, Ruslan MS, Aziz AH, Irwansyah N. Extraction and identification of bioactive compounds from agarwood leaves. IOP Conf Series Mater Sci Eng 162: 012028.
[http://dx.doi.org/10.1088/1757-899X/162/1/012028]
[16]
Alara OR, Abdurahman NH, Ukaegbu CI, Azhari NH. Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique. Ind Crops Prod 2018; 122: 533-44.
[http://dx.doi.org/10.1016/j.indcrop.2018.06.034]
[17]
Saravanan S, Parimelazhagan T. In vitro antioxidant, antimicrobial and anti-diabetic properties of polyphenols of Passiflora ligularis Juss. fruit pulp. Food Sci Hum Wellness 2014; 3(2): 56-64.
[http://dx.doi.org/10.1016/j.fshw.2014.05.001]
[18]
Oriakhi K, Oikeh EI, Ezeugwu N, et al. Comparative antioxidant activities of extracts of Vernonia amygdalina and Ocimum gratissimum leaves. J Agric Sci 2013; 6(1): 13-20.
[19]
Alara OR, Abdurahman NH, Abdul Mudalip SK, et al. Effect of drying methods on the free radicals scavenging activity of Vernonia amygdalina growing in Malaysia. J King Saud Univ Sci 2017; 32(4): 495-9.
[20]
Kothari S, Mishra V, Bharat S, Tonpay SD. Antimicrobial activity and phytochemical screening of serial extracts from leaves of Aegle marmelos (Linn.). Acta Pol Pharm 2011; 68(5): 687-92.
[PMID: 21928713]
[21]
Chen SL, Yu H, Luo HM, Wu Q, Li CF, Steinmetz A. Conservation and sustainable use of medicinal plants: Problems, progress, and prospects. Chin Med 2016; 11(1): 37.
[http://dx.doi.org/10.1186/s13020-016-0108-7] [PMID: 27478496]
[22]
Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev 2009; 2(5): 270-8.
[http://dx.doi.org/10.4161/oxim.2.5.9498] [PMID: 20716914]
[23]
Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 2010; 4(8): 118-26.
[http://dx.doi.org/10.4103/0973-7847.70902] [PMID: 22228951]
[24]
Abd Rani NZ, Husain K, Kumolosasi E. Moringa Genus: A Review of Phytochemistry and Pharmacology. Front Pharmacol 2018; 9: 108.
[http://dx.doi.org/10.3389/fphar.2018.00108] [PMID: 29503616]
[25]
Kumar N, Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep (Amst) 2019; 24e00370.
[http://dx.doi.org/10.1016/j.btre.2019.e00370] [PMID: 31516850]
[26]
Singh A, Mishra JN, Singh SK. Pharmacological importance of Moringa concanensis Nimmo leaf: An overview. Asian J Pharm Clin Res 2019; 12(2): 27-31.
[http://dx.doi.org/10.22159/ajpcr.2019.v12i2.28979]
[27]
Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines 2018; 5(3): 93.
[http://dx.doi.org/10.3390/medicines5030093] [PMID: 30149600]
[28]
Lin D, Xiao M, Zhao J, et al. An overview of plant phenolic compounds and their importance in human nutrition and management of Type 2 Diabetes. Molecules 2016; 21(10): 1374.
[http://dx.doi.org/10.3390/molecules21101374] [PMID: 27754463]
[29]
Tatke P, Jaiswal Y. An overview of microwave assisted extraction and its applications in herbal research. Res J Med Plant 2011; 5(1): 21-31.
[http://dx.doi.org/10.3923/rjmp.2011.21.31]
[30]
Rahman MM, Rahaman MS, Islam MR, et al. Role of phenolic compounds in human disease: Current knowledge and future prospects. Molecules 2021; 27(1): 233.
[http://dx.doi.org/10.3390/molecules27010233] [PMID: 35011465]
[31]
Gori A, Brunetti C, dos Santos Nascimento LB, et al. Photoprotective role of photosynthetic and non-photosynthetic pigments in phillyrea latifolia: Is their “antioxidant” function prominent in leaves exposed to severe summer drought? Int J Mol Sci 2021; 22(15): 8303.
[http://dx.doi.org/10.3390/ijms22158303] [PMID: 34361067]
[32]
Santhanam R, Ahmad S, Abas F, Ismail IS, Rukayadi Y, Shaari K. Photoprotective properties of Zanthoxylum rhetsa: An in vitro analysis. J Chem Pharm Res 2013; 5(12): 1512-20.

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