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Anti-Cancer Agents in Medicinal Chemistry

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ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

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Research Article

Enrichment of Isaria felina Culture with Selenium Enhances its in vivo Antitumor Effects on H22 Hepatoma via Decreasing the Expression of VEGF

Author(s): Xihua Yang*, Yongming Yang, Lili Zhao, Lixia Chen, Jing Wang, Lei Yan and Jinfeng Ma*

Volume 23, Issue 14, 2023

Published on: 30 May, 2023

Page: [1689 - 1696] Pages: 8

DOI: 10.2174/1871520623666230505094746

Price: $65

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Abstract

Background: The polysaccharide extract of C. sinensis, Isaria felina (IF), has antitumor effects. Selenium (Se) can improve disease prevention and reduce the toxicity of toxic elements, but the effect of Se-enriched IF on hepatoma remains unknown.

Objective: To determine the organic transformation of Se and compare the antitumor effects between Se-enriched IF (IF-Se) and IF on xenograft H22 hepatoma-bearing mice.

Methods: Se was added to the solid-state culture medium, and the organic Se content was detected by HPLC-ICP-MS. Forty-two Kunming mice were randomly divided into seven groups to test the antitumor effects of low- (300 mg/kg) and high- (600 mg/kg) doses of IF-Se and IF through xenograft. Huai’er granules were administered as the positive control. In addition, interleukin (IL)-2 and vascular endothelial growth factor (VEGF) expressions were measured by enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry method.

Results: The conversion rate in the IF-Se70, IF-Se140, and IF-Se280 groups were 91.5%, 93.4%, and 89.3%, respectively. Therefore, IF-Se140 was used to carry out the subsequent experiments. The tumor inhibition rates of IF-Se were significantly higher compared with IF (P < 0.05). Moreover, the spleen coefficient, IL-2, and VEGF expression levels significantly decreased (all Ps < 0.05), and the thymus coefficient significantly increased (P < 0.05) in the high-dose IF-Se group compared with the model control group.

Conclusion: The inhibitory effects of IF on H22 hepatoma-bearing mice were enhanced after Se enrichment. Therefore, Se-enriched IF might be a new strategy for treating hepatoma.

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[1]
Yang, X.; Zhang, S.; Ren, L.; Zhang, H.; Bai, X. Nephroprotective effects of Isaria felina in rats with adenine-induced chronic renal failure. J. Pharm. Pharmacol., 2013, 65(9), 1409-1418.
[http://dx.doi.org/10.1111/jphp.12110] [PMID: 23927479]
[2]
Bao, K.; Wang, X.; Wang, K.; Yang, Y.; Li, G. Effects of dietary supplementation with selenium and vitamin E on growth performance, nutrient apparent digestibility and blood parameters in female Sika deer (Cervus nippon). Biol. Trace Elem. Res., 2020, 195(2), 454-460.
[http://dx.doi.org/10.1007/s12011-019-01856-7] [PMID: 31452087]
[3]
Camara, A.Y.; Wan, Y.; Yu, Y.; Wang, Q.; Wang, K.; Li, H. Effect of endogenous selenium on arsenic uptake and antioxidative enzymes in As-exposed rice seedlings. Int. J. Environ. Res. Public Health, 2019, 16(18), 3350.
[http://dx.doi.org/10.3390/ijerph16183350] [PMID: 31514288]
[4]
Cámara-Martos, F.; Ramírez-Ojeda, A.M.; Jiménez-Mangas, M.; Sevillano-Morales, J.; Moreno-Rojas, R. Selenium and cadmium in bioaccessible fraction of organic weaning food: Risk assessment and influence of dietary components. J. Trace Elem. Med. Biol., 2019, 56, 116-123.
[http://dx.doi.org/10.1016/j.jtemb.2019.08.004] [PMID: 31445186]
[5]
Fernandes, A.P.; Gandin, V. Selenium compounds as therapeutic agents in cancer. Biochim. Biophys. Acta, Gen. Subj., 2015, 1850(8), 1642-1660.
[http://dx.doi.org/10.1016/j.bbagen.2014.10.008] [PMID: 25459512]
[6]
Labunskyy, V.M.; Hatfield, D.L.; Gladyshev, V.N. Selenoproteins: Molecular pathways and physiological roles. Physiol. Rev., 2014, 94(3), 739-777.
[http://dx.doi.org/10.1152/physrev.00039.2013] [PMID: 24987004]
[7]
Fairweather-Tait, S.J.; Collings, R.; Hurst, R. Selenium bioavailability: Current knowledge and future research requirements. Am. J. Clin. Nutr., 2010, 91(5), 1484S-1491S.
[http://dx.doi.org/10.3945/ajcn.2010.28674J] [PMID: 20200264]
[8]
Ferreira, R.L.U.; Sena-Evangelista, K.C.M.; de Azevedo, E.P.; Pinheiro, F.I.; Cobucci, R.N.; Pedrosa, L.F.C. Selenium in human health and gut microflora: Bioavailability of selenocompounds and relationship with diseases. Front. Nutr., 2021, 8685317
[http://dx.doi.org/10.3389/fnut.2021.685317] [PMID: 34150830]
[9]
Kang, D.; Lee, J.; Wu, C.; Guo, X.; Lee, B.J.; Chun, J.S.; Kim, J.H. The role of selenium metabolism and selenoproteins in cartilage homeostasis and arthropathies. Exp. Mol. Med., 2020, 52(8), 1198-1208.
[http://dx.doi.org/10.1038/s12276-020-0408-y] [PMID: 32788658]
[10]
Misra, S.; Boylan, M.; Selvam, A.; Spallholz, J.; Björnstedt, M. Redox-active selenium compounds-from toxicity and cell death to cancer treatment. Nutrients, 2015, 7(5), 3536-3556.
[http://dx.doi.org/10.3390/nu7053536] [PMID: 25984742]
[11]
Chen, S.; Xing, C.; Huang, D.; Zhou, C.; Ding, B.; Guo, Z.; Peng, Z.; Wang, D.; Zhu, X.; Liu, S.; Cai, Z.; Wu, J.; Zhao, J.; Wu, Z.; Zhang, Y.; Wei, C.; Yan, Q.; Wang, H.; Fan, D.; Liu, L.; Zhang, H.; Cao, Y. Eradication of tumor growth by delivering novel photothermal selenium-coated tellurium nanoheterojunctions. Sci. Adv., 2020, 6(15)eaay6825
[http://dx.doi.org/10.1126/sciadv.aay6825] [PMID: 32284997]
[12]
Chen, Y.C.; Prabhu, K.S.; Das, A.; Mastro, A.M. Dietary selenium supplementation modifies breast tumor growth and metastasis. Int. J. Cancer, 2013, 133(9), 2054-2064.
[http://dx.doi.org/10.1002/ijc.28224] [PMID: 23613334]
[13]
Gandhi, U.H.; Kaushal, N.; Hegde, S.; Finch, E.R.; Kudva, A.K.; Kennett, M.J.; Jordan, C.T.; Paulson, R.F.; Prabhu, K.S. Selenium suppresses leukemia through the action of endogenous eicosanoids. Cancer Res., 2014, 74(14), 3890-3901.
[http://dx.doi.org/10.1158/0008-5472.CAN-13-3694] [PMID: 24872387]
[14]
Yang, X.; Chen, L.; Zhao, L.; Yang, Y.; Wang, J.; Yan, L.; Tai, G.; Zhang, H. Cordyceps sinensis-derived fungus Isaria felina ameliorates experimental autoimmune thyroiditis in mice. Biomed. Pharmacother., 2021, 140111733
[http://dx.doi.org/10.1016/j.biopha.2021.111733] [PMID: 34029950]
[15]
Montesbayón, M.; Molet, M.; González, E.; Sanzmedel, A. Evaluation of different sample extraction strategies for selenium determination in selenium-enriched plants (Allium sativum and Brassica juncea) and Se speciation by HPLC-ICP-MS. Talanta, 2006, 68(4), 1287-1293.
[http://dx.doi.org/10.1016/j.talanta.2005.07.040] [PMID: 18970462]
[16]
Vacchina, V.; Foix, D.; Menta, M.; Martinez, H.; Séby, F. Optimization of elemental selenium (Se(0)) determination in yeasts by anion-exchange HPLC-ICP-MS. Anal. Bioanal. Chem., 2021, 413(7), 1809-1816.
[http://dx.doi.org/10.1007/s00216-020-03129-y] [PMID: 33527180]
[17]
Zhong, S.S.; Xiang, Y.J.; Liu, P.J.; He, Y.; Yang, T.T.; Wang, Y.Y.; Rong, A.; Zhang, J.; Liu, G.Z. Effect of Cordyceps sinensis on the treatment of experimental autoimmune encephalomyelitis: A pilot study on mice model. Chin. Med. J., 2017, 130(19), 2296-2301.
[PMID: 28937034]
[18]
Chen, Y.C.; Chen, Y.H.; Pan, B.S.; Chang, M.M.; Huang, B.M. Functional study of Cordyceps sinensis and cordycepin in male reproduction: A review. Yao Wu Shi Pin Fen Xi, 2017, 25(1), 197-205.
[PMID: 28911537]
[19]
Chen, Y.C.; Huang, B.M. Regulatory mechanisms of Cordyceps sinensis on steroidogenesis in MA-10 mouse Leydig tumor cells. Biosci. Biotechnol. Biochem., 2010, 74(9), 1855-1859.
[http://dx.doi.org/10.1271/bbb.100262] [PMID: 20834160]
[20]
Kiełczykowska, M.; Polz-Dacewicz, M.; Kopciał E.; Mitrus, O.; Kurzepa, J.; Marzec, Z.; Musik, I. Selenium prevents lithium accumulation and does not disturb basic microelement homeostasis in liver and kidney of rats exposed to lithium. Ann. Agric. Environ. Med., 2020, 27(1), 129-133.
[http://dx.doi.org/10.26444/aaem/105926] [PMID: 32208591]
[21]
Rao, S.; Lin, Y.; Du, Y.; He, L.; Huang, G.; Chen, B.; Chen, T. Designing multifunctionalized selenium nanoparticles to reverse oxidative stress-induced spinal cord injury by attenuating ROS overproduction and mitochondria dysfunction. J. Mater. Chem. B Mater. Biol. Med., 2019, 7(16), 2648-2656.
[http://dx.doi.org/10.1039/C8TB02520G] [PMID: 32254998]
[22]
Saeed Ali, M.; Hussein, M.R.; Kandeil, A.M. The pro-oxidant, apoptotic and anti-angiogenic effects of selenium supplementation on colorectal tumors induced by 1, 2-dimethylhydrazine in BALB/C mice. Rep. Biochem. Mol. Biol., 2019, 8(3), 216-226.
[PMID: 32274393]
[23]
Bjørklund, G.; Shanaida, M.; Lysiuk, R.; Antonyak, H.; Klishch, I.; Shanaida, V.; Peana, M. Selenium: An antioxidant with a critical role in anti-aging. Molecules, 2022, 27(19), 6613.
[http://dx.doi.org/10.3390/molecules27196613] [PMID: 36235150]
[24]
Cosín-Tomàs, M.; Senserrich, J.; Arumí-Planas, M.; Alquézar, C.; Pallàs, M.; Martín-Requero, Á.; Suñol, C.; Kaliman, P.; Sanfeliu, C. Role of resveratrol and selenium on oxidative stress and expression of antioxidant and anti-aging genes in immortalized lymphocytes from alzheimer’s disease patients. Nutrients, 2019, 11(8), 1764.
[http://dx.doi.org/10.3390/nu11081764] [PMID: 31370365]
[25]
Kieliszek, M.; Bano, I.; Zare, H. A comprehensive review on selenium and its effects on human health and distribution in middle eastern countries. Biol. Trace Elem. Res., 2022, 200(3), 971-987.
[http://dx.doi.org/10.1007/s12011-021-02716-z] [PMID: 33884538]
[26]
Pi, J.; Shen, L.; Yang, E.; Shen, H.; Huang, D.; Wang, R.; Hu, C.; Jin, H.; Cai, H.; Cai, J.; Zeng, G.; Chen, Z.W. Macrophage-targeted isoniazid-selenium nanoparticles promote antimicrobial immunity and synergize bactericidal destruction of Tuberculosis bacilli. Angew. Chem. Int. Ed., 2020, 59(8), 3226-3234.
[http://dx.doi.org/10.1002/anie.201912122] [PMID: 31756258]
[27]
Qian, F.; Misra, S.; Prabhu, K.S. Selenium and selenoproteins in prostanoid metabolism and immunity. Crit. Rev. Biochem. Mol. Biol., 2019, 54(6), 484-516.
[http://dx.doi.org/10.1080/10409238.2020.1717430] [PMID: 31996052]
[28]
Shang, D.; Li, Y.; Wang, C.; Wang, X.; Yu, Z.; Fu, X. A novel polysaccharide from Se-enriched Ganoderma lucidum induces apoptosis of human breast cancer cells. Oncol. Rep., 2011, 25(1), 267-272.
[PMID: 21109986]
[29]
Wang, Y.; Chen, J.; Zhang, D.; Zhang, Y.; Wen, Y.; Li, L.; Zheng, L. Tumoricidal effects of a selenium (Se)-polysaccharide from Ziyang green tea on human osteosarcoma U-2 OS cells. Carbohydr. Polym., 2013, 98(1), 1186-1190.
[http://dx.doi.org/10.1016/j.carbpol.2013.07.022] [PMID: 23987462]
[30]
Li, W.; Guo, M.; Liu, Y.; Mu, W.; Deng, G.; Li, C.; Qiu, C. Selenium induces an antitumor effect via inhibiting intratumoral angiogenesis in a mouse model of transplanted canine mammary tumor cells. Biol. Trace Elem. Res., 2016, 171(2), 371-379.
[http://dx.doi.org/10.1007/s12011-015-0554-6] [PMID: 26507439]
[31]
Bhattacharya, A.; Turowski, S.G.; San Martin, I.D.; Rajput, A.; Rustum, Y.M.; Hoffman, R.M.; Seshadri, M. Magnetic resonance and fluorescence-protein imaging of the anti-angiogenic and anti-tumor efficacy of selenium in an orthotopic model of human colon cancer. Anticancer Res., 2011, 31(2), 387-393.
[PMID: 21378316]
[32]
Lala, V.; Zubair, M.; Minter, D.A. Liver Function Tests; StatPearls: Treasure Island, FL, 2022. Available from: https://www.ncbi.nlm.nih.gov/books/NBK482489/
[33]
Sarkar, J.; Kumari, J.; Tonello, J.M.; Kamihira, M.; Kumar, A. Enhanced hepatic functions of genetically modified mouse hepatoma cells by spheroid culture for drug toxicity screening. Biotechnol. J., 2017, 12(10)1700274
[http://dx.doi.org/10.1002/biot.201700274] [PMID: 28834334]
[34]
Suzuki, G.; Nakamura, M.; Michinaka, C.; Tue, N.M.; Handa, H.; Takigami, H. Corrigendum to “Separate screening of brominated and chlorinated dioxins in field samples using in vitro reporter gene assays with rat and mouse hepatoma cell lines” (ACA 975 (2017) 86-95). Anal. Chim. Acta, 2017, 984, 232-235.
[http://dx.doi.org/10.1016/j.aca.2017.06.003] [PMID: 28843568]
[35]
Tan, J.; Shen, W.; Shi, W.; Chen, X.; Sun, D.; Xu, C.; Yan, Q.; Cheng, H.; Lai, Y.; Ji, H. ONTD induces growth arrest and apoptosis of human hepatoma Bel-7402 cells though a peroxisome proliferator-activated receptor γ-dependent pathway. Toxicol. In Vitro, 2017, 45(Pt 1), 44-53.
[http://dx.doi.org/10.1016/j.tiv.2017.08.012] [PMID: 28834734]

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