Abstract
Background: Multi-drug resistance of Pseudomonas aeruginosa comprises the most vital obstacles in the antibacterial fight worldwide. The innovation of novel and effective antibacterial and/or resistance modulators is crucial to variance the extent of resistance or to reverse multidrug resistance.
Objective: This study aimed to determine the in vitro antibacterial activity of the F.carica against the Pseudomonas aeruginosa associated with nosocomial infection.
Methods: We studied different extracts of F.carica in methanol, benzene and water with antibiotics and their synergistic effect against P. aeruginosa using minimum inhibitory concentration and fraction inhibitory concentration index.
Results: Our results revealed that the methanol extract of leaves possessed maximum phyto constituents and were active against P. aeruginosa. Methanol extracts of leaves alone and in combination with antibiotics showed a higher zone of inhibition and were synergistic (<0.5) compared to other extracts. Moreover, the combination of ofloxacin with all solvent extracts enhanced the synergistic antibacterial activity with respect to other antibiotics used.
Conclusion: We explored the synergistic effects of F. carica methanol extracts alone and with antibiotics was the most potential against P. aeruginosa.
Graphical Abstract
[1]
Hirsch EB, Tam VH. Impact of multidrug-resistant Pseudomonas aeruginosa infection on patient outcomes. Expert Rev Pharmacoecon Outcomes Res 2010; 10(4): 441-51.
[http://dx.doi.org/10.1586/erp.10.49] [PMID: 20715920]
[http://dx.doi.org/10.1586/erp.10.49] [PMID: 20715920]
[2]
Vieira RGL, Moraes TS, Silva LO, et al. In vitro studies of the antibacterial activity of Copaifera spp. oleoresins, sodium hypochlorite, and peracetic acid against clinical and environmental isolates recovered from a hemodialysis unit. Antimicrob Resist Infect Control 2018; 7(1): 14.
[http://dx.doi.org/10.1186/s13756-018-0307-3] [PMID: 29410782]
[http://dx.doi.org/10.1186/s13756-018-0307-3] [PMID: 29410782]
[3]
Jiménez-Guerra G, Heras-Cañas V, Gutiérrez-Soto M, et al. Urinary tract infection by Acinetobacter baumannii and Pseudomonas aeruginosa: Evolution of antimicrobial resistance and therapeutic alternatives. J Med Microbiol 2018; 67(6): 790-7.
[http://dx.doi.org/10.1099/jmm.0.000742] [PMID: 29693543]
[http://dx.doi.org/10.1099/jmm.0.000742] [PMID: 29693543]
[4]
Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: Current methods and future trends. Afr J Biotechnol 2008; 7(12): 1797-806.
[http://dx.doi.org/10.5897/AJB07.613]
[http://dx.doi.org/10.5897/AJB07.613]
[5]
Teklehaymanot T. An ethnobotanical survey of medicinal and edible plants of Yalo Woreda in Afar regional state, Ethiopia. J Ethnobiol Ethnomed 2017; 13(1): 40.
[http://dx.doi.org/10.1186/s13002-017-0166-7] [PMID: 28679438]
[http://dx.doi.org/10.1186/s13002-017-0166-7] [PMID: 28679438]
[6]
Badgujar SB, Patel VV, Bandivdekar AH, Mahajan RT. Traditional uses, phytochemistry and pharmacology of Ficus carica: A review. Pharm Biol 2014; 52(11): 1487-503.
[http://dx.doi.org/10.3109/13880209.2014.892515] [PMID: 25017517]
[http://dx.doi.org/10.3109/13880209.2014.892515] [PMID: 25017517]
[7]
Barolo MI, Ruiz Mostacero N, López SN. Ficus carica L. (Moraceae): An ancient source of food and health. Food Chem 2014; 164: 119-27.
[http://dx.doi.org/10.1016/j.foodchem.2014.04.112] [PMID: 24996314]
[http://dx.doi.org/10.1016/j.foodchem.2014.04.112] [PMID: 24996314]
[8]
Jeong MR, Kim HY, Cha JD. Antimicrobial activity of methanol extract from Ficus carica leaves against oral bacteria. J Bacteriol Virol 2009; 39(2): 97-102.
[http://dx.doi.org/10.4167/jbv.2009.39.2.97]
[http://dx.doi.org/10.4167/jbv.2009.39.2.97]
[9]
Lee YS, Cha JD. Synergistic antibacterial activity of fig (Ficus carica) leaves extract against clinical isolates of methicillin-resistant Staphylococcus aureus. Microbiol Biotechnol Lett 2010; 38(4): 405-13.
[10]
Kokate CK, Khandelwal KR, Pawar AP, Gohalz SB. Red Odourless Tasteless Fine Powder Insoluble in water, organic solvents and acids but sparingly soluble in aqua regia. Vallabh Prakashan Practical Pharmacognosy New Delhi 1991; (3): 107-11.
[11]
Zaidi KU, Shah F, Parmar R, Thawani V. Anticandidal synergistic activity of Ocimum sanctum and fluconazole of azole resistance strains of clinical isolates. J Mycol Med 2018; 28(2): 289-93.
[http://dx.doi.org/10.1016/j.mycmed.2018.04.004] [PMID: 29730129]
[http://dx.doi.org/10.1016/j.mycmed.2018.04.004] [PMID: 29730129]
[12]
Shah F, Hasan Z, Zaidi KU. Phytochemical constituents and synergistic activity of Olea Europaea plant extracts against some human disease-causing species. Int J Innov Res Adv Stud 2016; 3: 127-32.
[13]
Shah F, Hasan Z, Zaidi KU. Evaluation of phyto constituent and synergistic antibacterial activity of Ocimum sanctum extract against some gram-positive and gram-negative species. Biosci Biotechnol Res Commun 2016; 9(4): 856-64.
[http://dx.doi.org/10.21786/bbrc/9.4/41]
[http://dx.doi.org/10.21786/bbrc/9.4/41]
[14]
Zaidi KU, Mani A, Thawani V, Mehra A. Total protein profile and drug resistance in Candida albicans isolated from clinical samples. Mol Biol Int 2016; 20164982131
[15]
Rakholiya KD, Kaneria MJ, Chanda SV. In vitro assessment of novel antimicrobial from methanol extracts of matured seed kernel and leaf of Mangifera indica L.(Kesar Mango) for inhibition of Pseudomonas spp. and their synergistic potential. American Journal of Drug Discovery and Development 2014; 5(1): 13-23.
[http://dx.doi.org/10.3923/ajdd.2015.13.23]
[http://dx.doi.org/10.3923/ajdd.2015.13.23]
[16]
Bacha K, Tariku Y, Gebreyesus F, et al. Antimicrobial and anti-Quorum Sensing activities of selected medicinal plants of Ethiopia: Implication for development of potent antimicrobial agents. BMC Microbiol 2016; 16(1): 139.
[http://dx.doi.org/10.1186/s12866-016-0765-9] [PMID: 27400878]
[http://dx.doi.org/10.1186/s12866-016-0765-9] [PMID: 27400878]
[17]
Anyanwu M, Okoye R. Antimicrobial activity of Nigerian medicinal plants. J Intercult Ethnopharmacol 2017; 6(2): 1.
[http://dx.doi.org/10.5455/jice.20170106073231] [PMID: 28512606]
[http://dx.doi.org/10.5455/jice.20170106073231] [PMID: 28512606]
[18]
Fluit AC, Visser MR, Schmitz FJ. Molecular detection of antimicrobial resistance. Clin Microbiol Rev 2001; 14(4): 836-71.
[http://dx.doi.org/10.1128/CMR.14.4.836-871.2001] [PMID: 11585788]
[http://dx.doi.org/10.1128/CMR.14.4.836-871.2001] [PMID: 11585788]
[19]
Bittner Fialová S, Rendeková K. Mučaji P, Nagy M, Slobodníková L. Antibacterial activity of medicinal plants and their constituents in the context of skin and wound infections, considering european legislation and folk medicine—a review. Int J Mol Sci 2021; 22(19): 10746.
[http://dx.doi.org/10.3390/ijms221910746] [PMID: 34639087]
[http://dx.doi.org/10.3390/ijms221910746] [PMID: 34639087]
[20]
Teixeira DM, Patão RF, Coelho AV, da Costa CT. Comparison between sample disruption methods and Solid–Liquid Extraction (SLE) to extract phenolic compounds from Ficus carica leaves. J Chromatogr A 2006; 1103(1): 22-8.
[http://dx.doi.org/10.1016/j.chroma.2005.11.047] [PMID: 16343519]
[http://dx.doi.org/10.1016/j.chroma.2005.11.047] [PMID: 16343519]
[21]
Solomon A, Golubowicz S, Yablowicz Z, et al. Antioxidant activities and anthocyanin content of fresh fruits of common fig (Ficus carica L.). J Agric Food Chem 2006; 54(20): 7717-23.
[http://dx.doi.org/10.1021/jf060497h] [PMID: 17002444]
[http://dx.doi.org/10.1021/jf060497h] [PMID: 17002444]
[22]
AL-Ani I. Zimmermann S, Reichling J, Wink M. Antimicrobial activities of European propolis collected from various geographic origins alone and in combination with antibiotics. Medicines 2018; 5(1): 2.
[http://dx.doi.org/10.3390/medicines5010002] [PMID: 29301368]
[http://dx.doi.org/10.3390/medicines5010002] [PMID: 29301368]
[23]
Adwan G, Abu-Shanab B, Adwan K. Antibacterial activities of some plant extracts alone and in combination with different antimicrobials against multidrug–resistant Pseudomonas aeruginosa strains. Asian Pac J Trop Med 2010; 3(4): 266-9.
[http://dx.doi.org/10.1016/S1995-7645(10)60064-8]
[http://dx.doi.org/10.1016/S1995-7645(10)60064-8]
[24]
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999; 12(4): 564-82.
[http://dx.doi.org/10.1128/CMR.12.4.564] [PMID: 10515903]
[http://dx.doi.org/10.1128/CMR.12.4.564] [PMID: 10515903]
[25]
Horiuchi K, Shiota S, Hatano T, Yoshida T, Kuroda T, Tsuchiya T. Antimicrobial activity of oleanolic acid from Salvia officinalis and related compounds on vancomycin-resistant enterococci (VRE). Biol Pharm Bull 2007; 30(6): 1147-9.
[http://dx.doi.org/10.1248/bpb.30.1147] [PMID: 17541170]
[http://dx.doi.org/10.1248/bpb.30.1147] [PMID: 17541170]
[26]
Chandar B, Poovitha S, Ilango K. MohanKumar R, Parani M. Inhibition of New Delhi metallo-β-lactamase 1 (NDM-1) producing Escherichia coli IR-6 by selected plant extracts and their synergistic actions with antibiotics. Front Microbiol 2017; 8: 1580.
[http://dx.doi.org/10.3389/fmicb.2017.01580] [PMID: 28878746]
[http://dx.doi.org/10.3389/fmicb.2017.01580] [PMID: 28878746]
[27]
Mahomoodally MF, Dilmohamed S. Antibacterial and antibiotic potentiating activity of Vangueria madagascariensis leaves and ripe fruit pericarp against human pathogenic clinical bacterial isolates. J Tradit Complement Med 2016; 6(4): 399-403.
[http://dx.doi.org/10.1016/j.jtcme.2015.09.002] [PMID: 27774426]
[http://dx.doi.org/10.1016/j.jtcme.2015.09.002] [PMID: 27774426]
