Abstract
A deacetyl-thymosin cx1analogue containing four arginines at positions 14, 17, 19, and 20, instead of four lysines was synthesized by the manual solid-phase method. The synthetic [Arg14 17 19 20 deacetyl-thymosin cx1 and deacetyl-thymosin cx1 were tested for the effect on impaired T-lymphocyte transformation by phytohemagglutinin in uremic patients suffering from recurrent infectious diseases. The restoring activity on the impaired phytohemagglutinin stimulation of T-lymphocytes was obtained after incubation of peripheral lymphocytes isolated from uremic patients with the synthetic [Arg14•17•19•20] deacetyl-thymosin cx1. This analogue exhibited a far stronger restoring effect than that of our synthetic deacetyl-thymosin ex1. Abbreviations used: Obzl, benzyl estrer; Bzl, benzyl; Boe, tert-butoxycarbonyl; Mts, mesitylene-2-sulfonyl; DCC, N, N' -dicyclohexylcarbodiimide; HOBT, 1-hydro-xybenzotriazole; DMF, dimethylformamide; Et3N, triethylamine; AcOH, acetic acid; TFA, trifluoroacetic acid; TLC, thin-layer chromatography; HPLC, high performance liquid chromatography; PHA, phytohemagglutinin; SDS, sodium dodecyl sulfate; PBS, phosphate buffered saline; RPMI, Rosewell Park Memorial Institute; FCS, fetal calf serum; E-rosette, a rosette with sheep erythrocytes.
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