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Current Drug Research Reviews

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ISSN (Print): 2589-9775
ISSN (Online): 2589-9783

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Research Article

Evaluation of Hepatoprotective Efficacy of a Polyherbal Unani Formulation Majoon-Najah against Cadmium Chloride-induced Liver Damage

Author(s): Neelam Singh, Puneet Gupta*, Charu Bharti and Sadish Kumar Shanmugam

Volume 15, Issue 2, 2023

Published on: 11 January, 2023

Page: [188 - 198] Pages: 11

DOI: 10.2174/2589977515666221207141645

Price: $65

Abstract

Background: Majoon-Najah is a composite Unani formulation that consists of multiple medicinal plants and is advised for neurological illnesses. Several studies were carried out on Majoon-Najah (MN) and its ingredients to evaluate the protective effect against seizure and antidepressant activity in animals using a classical form as well as extract. Terminalia bellerica and Emblica officinalis are the major constituents of MN. Scientifically documented literature summarises the hepatoprotective potential of these constituents.

Aim: The current study aimed to evaluate the possible hepatoprotective, antioxidant and antiinflammatory perspective of traditional Indian Unani formulation MN and Majoon-Najah hydroalcoholic extract (MNHE) in a Guinea pig model.

Methods: Thirty adult male albino guinea pigs were randomly assigned into five groups for this study. MN and MNHE were given intragastrically for 15 days, followed by intraperitoneal Cadmium chloride (CdCl2, 3 mg/kg/day) from days 8 to 15, as per the schedule. Blood samples were taken from the heart on the 16th day, and the liver was operated on for biochemical analysis and histopathology under complete anesthesia.

Results: CdCl2 changed the levels of liver function markers, serum biochemical indicators like albumin, total protein, glucose, and cholesterol in the blood; lipid peroxidation (MDA), glutathione reductase (GSH), superoxide dismutase (SOD), and glutathione peroxidase (GPX) in hepatic tissue homogenate, pro-inflammatory cytokines level and liver cytoarchitecture. MN and MNHE were found to protect guinea pigs’ liver from CdCl2-induced injury by lowering raised parameters and increasing enzymatic antioxidants. MN and MNHE did not significantly heal injured liver tissues caused by CdCl2 in histopathological examinations.

Conclusion: CdCl2 induces hepatotoxicity that is likely to worsen with increasing dosage and duration of exposure. MN and MNHE exert their hepatoprotective action by scavenging free radicals, decreasing malondialdehyde levels, activating antioxidant enzymes, and down-regulating proinflammatory indicators.

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[1]
Shakya AK, Saxena M, Sharma N, Shrivastava S, Shukla S. Hepatoprotective efficacy of Sharbat-e-Deenar against carbon tetrachloride-induced liver damage. J Environ Pathol Toxicol Oncol 2012; 31(2): 131-41.
[http://dx.doi.org/10.1615/JEnvironPatholToxicolOncol.v31.i2.50] [PMID: 23216638]
[2]
Urooj M, Husain GM, Nadeem M, Naikodi MAR, Alam M, Kazmi MH. Antiepileptic and antidepressant activity of Majoon Najah (A Traditional Unani Formulation) in experimental animals. Int J Pharm Investig 2020; 10(3): 396-401.
[http://dx.doi.org/10.5530/ijpi.2020.3.70]
[3]
Afrin Z, Siddiqui A, Jafri MA, Vohora D, Asif M. Anticonvulsant activity of majoon najah against PTZ induced seizure in swiss albino mice. International Journal of Scientific Research in Biological Sciences 2019; 5(6): 170-9.
[http://dx.doi.org/10.26438/ijsrbs/v5i6.170179]
[4]
Hua T, Zhang X, Tang B, et al. Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. BMC Vet Res 2018; 14(1): 138.
[http://dx.doi.org/10.1186/s12917-018-1457-5] [PMID: 29699558]
[5]
Sobeh M, Mahmoud MF, Hasan RA, et al. Chemical composition, antioxidant and hepatoprotective activities of methanol extracts from leaves of Terminalia bellirica and Terminalia sericea (Combretaceae). PeerJ 2019; 7: e6322.
[http://dx.doi.org/10.7717/peerj.6322] [PMID: 30834179]
[6]
Gupta A, Pandey AK. Aceclofenac-induced hepatotoxicity: An ameliorative effect of Terminalia bellirica fruit and ellagic acid. World J Hepatol 2020; 12(11): 949-64.
[http://dx.doi.org/10.4254/wjh.v12.i11.949] [PMID: 33312421]
[7]
Sinha R, Jindal R, Faggio C. Protective effect of emblica officinalis in cyprinus carpio against hepatotoxicity induced by malachite green: Ultrastructural and molecular analysis. Appl Sci (Basel) 2021; 11(8): 3507.
[http://dx.doi.org/10.3390/app11083507]
[8]
Singh D, Cho WC, Upadhyay G. Drug-induced liver toxicity and prevention by herbal antioxidants: An overview. Front Physiol 2016; 6: 363.
[http://dx.doi.org/10.3389/fphys.2015.00363] [PMID: 26858648]
[9]
Damodara Reddy V, Padmavathi P, Gopi S, Paramahamsa M, Varadacharyulu NC. Protective effect of emblica officinalis against alcohol-induced hepatic injury by ameliorating oxidative stress in rats. Indian J Clin Biochem 2010; 25(4): 419-24.
[http://dx.doi.org/10.1007/s12291-010-0058-2] [PMID: 21966117]
[10]
Shen T, Liu Y, Shang J, et al. Incidence and etiology of drug-induced liver injury in mainland China. Gastroenterology 2019; 156(8): 2230-2241.e11.
[http://dx.doi.org/10.1053/j.gastro.2019.02.002] [PMID: 30742832]
[11]
Al-Baqami N, Hamza R. Protective effect of resveratrol against hepatotoxicity of cadmium in male rats: Antioxidant and histopathological approaches. Coatings 2021; 11(5): 594.
[http://dx.doi.org/10.3390/coatings11050594]
[12]
Zhao L, Duan Z, Wang Y, et al. Protective effect of Terminalia chebula Retz. extract against Aβ aggregation and Aβ-induced toxicity in Caenorhabditis elegans. J Ethnopharmacol 2021; 268: 113640.
[http://dx.doi.org/10.1016/j.jep.2020.113640] [PMID: 33307058]
[13]
Abdel-Emam RA, Ahmed EA, Ali MF. The protective role of lcarnitine against 1st- and 2nd-generation antihistamine-induced liver injury in mice. Comp Clin Pathol 2020; 29(1): 213-21.
[http://dx.doi.org/10.1007/s00580-019-03045-3]
[14]
Gouveia K, Hurst JL. Improving the practicality of using nonaversive handling methods to reduce background stress and anxiety in laboratory mice. Sci Rep 2019; 9(1): 20305.
[http://dx.doi.org/10.1038/s41598-019-56860-7] [PMID: 31889107]
[15]
Nair A, Jacob S. A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm 2016; 7(2): 27-31.
[http://dx.doi.org/10.4103/0976-0105.177703] [PMID: 27057123]
[16]
Oghenesuvwe EE. Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. J Nat Sci Res 2014; 4: 100-6.
[17]
Goodarzi Z, Karami E, Yousefi S, Dehdashti A, Bandegi AR, Ghanbari A. Hepatoprotective effect of atorvastatin on Cadmium chloride induced hepatotoxicity in rats. Life Sci 2020; 254: 117770.
[http://dx.doi.org/10.1016/j.lfs.2020.117770] [PMID: 32407846]
[18]
Baş H, Apaydın FG, Kalender S, Kalender Y. Lead nitrate and cadmium chloride induced hepatotoxicity and nephrotoxicity: Protective effects of sesamol on biochemical indices and pathological changes. J Food Biochem 2021; 45(7): e13769.
[http://dx.doi.org/10.1111/jfbc.13769] [PMID: 34021611]
[19]
Yang Z, He Y, Wang H, Zhang Q. Protective effect of melatonin against chronic cadmium-induced hepatotoxicity by suppressing oxidative stress, inflammation, and apoptosis in mice. Ecotoxicol Environ Saf 2021; 228: 112947.
[http://dx.doi.org/10.1016/j.ecoenv.2021.112947] [PMID: 34736034]
[20]
Li X, Li H, Cai D, et al. Chronic oral exposure to cadmium causes liver inflammation by NLRP3 inflammasome activation in pubertal mice. Food Chem Toxicol 2021; 148: 111944.
[http://dx.doi.org/10.1016/j.fct.2020.111944] [PMID: 33359024]
[21]
Mohammed KA, Goji ADT, Tanko Y, Muhammed A, Salisu IA. Protective effects of magnesium chloride on liver enzymes and biomarkers of oxidative stress in high fat diet fed rats. Niger J Physiol Sci 2019; 34(2): 149-57.
[PMID: 32343266]
[22]
Shabbir M, Afsar T, Razak S, Almajwal A, Khan MR. Phytochemical analysis and evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice. Lipids Health Dis 2020; 19(1): 46.
[http://dx.doi.org/10.1186/s12944-020-01231-9] [PMID: 32178678]
[23]
Hasan KMM, Tamanna N, Haque MA. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Sci Hum Wellness 2018; 7(1): 77-82.
[http://dx.doi.org/10.1016/j.fshw.2017.12.002]
[24]
Mahaldar K, Hossain A, Islam F, et al. Antioxidant and hepatoprotective activity of Piper retrofractum against Paracetamol-induced hepatotoxicity in Sprague-Dawley rat. Nat Prod Res 2020; 34(22): 3219-25.
[http://dx.doi.org/10.1080/14786419.2018.1550768] [PMID: 30663362]
[25]
Ojeaburu SI, Oriakhi K. Hepatoprotective, antioxidant and, antiinflammatory potentials of gallic acid in carbon tetrachloride-induced hepatic damage in Wistar rats. Toxicol Rep 2021; 8: 177-85.
[http://dx.doi.org/10.1016/j.toxrep.2021.01.001] [PMID: 33489777]
[26]
Masson-meyers DS, Andrade TAM, Caetano GF, et al. Experimental models and methods for cutaneous wound healing assessment. Int J Exp Pathol 2020; 101(1-2): 21-37.
[http://dx.doi.org/10.1111/iep.12346]
[27]
Chang Z, Jian P, Zhang Q, et al. Function tannins in Terminalia bellirica inhibit hepatocellular carcinoma growth by regulating. Food Func 2021; 3720-39.
[28]
Karamalakova YD, Nikolova GD, Georgiev TK, Gadjeva VG, Tolekova AN. Hepatoprotective properties of Curcuma longa L. extract in bleomycin-induced chronic hepatotoxicity. Drug Discov Ther 2019; 13(1): 9-16.
[http://dx.doi.org/10.5582/ddt.2018.01081] [PMID: 30880325]
[29]
Vairetti M, Di Pasqua LG, Cagna M, Richelmi P, Ferrigno A, Berardo C. Changes in glutathione content in liver diseases: An update. Antioxidants 2021; 10(3): 364.
[http://dx.doi.org/10.3390/antiox10030364] [PMID: 33670839]
[30]
Genzer SC, Huynh T, Coleman-Mccray JD, Harmon JR, Welch SR, Spengler JR. Hematology and clinical chemistry reference intervals for inbred strain 13/n Guinea Pigs (Cavia porcellus). J Am Assoc Lab Anim Sci 2019; 58(3): 293-303.
[http://dx.doi.org/10.30802/AALAS-JAALAS-18-000118] [PMID: 31010455]
[31]
Mumtaz S, Ali S, Khan R, et al. The protective role of ascorbic acid in the hepatotoxicity of cadmium and mercury in rabbits. Environ Sci Pollut Res Int 2019; 26(14): 14087-96.
[http://dx.doi.org/10.1007/s11356-019-04620-5] [PMID: 30852747]
[32]
Mashinchian O, Johari-Ahar M, Ghaemi B, Rashidi M, Barar J, Omidi Y. Impacts of quantum dots in molecular detection and bioimaging of cancer. Bioimpacts 2017; 4(3): 149-66.
[http://dx.doi.org/10.15171/bi.2014.008] [PMID: 25337468]
[33]
Pahlavani N, Malekahmadi M, Firouzi S, et al. Molecular and cellular mechanisms of the effects of propolis in inflammation. Oxidative Stress and Glycemic Control in Chronic Diseases. Nutr Metab 2020; 17: 65.
[34]
Sriuttha P, Sirichanchuen B, Permsuwan U. Hepatotoxicity of nonsteroidal anti-inflammatory drugs: A systematic review of randomized controlled trials. Int J Hepatol 2018; 2018: 5253623.
[http://dx.doi.org/10.1155/2018/5253623]
[35]
Refat MS, Hamza RZ, Adam AMA, et al. Antioxidant, antigenotoxic, and hepatic ameliorative effects of quercetin/zinc complex on cadmium-induced hepatotoxicity and alterations in hepatic tissue structure. Coatings 2021; 11(5): 501.
[http://dx.doi.org/10.3390/coatings11050501]
[36]
Alharthi WA, Hamza RZ, Elmahdi MM, Abuelzahab HSH, Saleh H. Selenium and L-carnitine ameliorate reproductive toxicity induced by cadmium in male mice. Biol Trace Elem Res 2020; 197(2): 619-27.
[http://dx.doi.org/10.1007/s12011-019-02016-7] [PMID: 31863275]
[37]
Roghani M, Kalantari H, Khodayar MJ, et al. Alleviation of liver dysfunction, oxidative stress and inflammation underlies the protective effect of ferulic acid in methotrexate-induced hepatotoxicity. Drug Des Devel Ther 2020; 14: 1933-41.
[http://dx.doi.org/10.2147/DDDT.S237107] [PMID: 32546960]
[38]
Shakya AK, Sharma N, Saxena M, Shrivastava S, Shukla S. Evaluation of the antioxidant and hepatoprotective effect of Majoon-e-Dabeed-ul-ward against carbon tetrachloride induced liver injury. Exp Toxicol Pathol 2012; 64(7-8): 767-73.
[http://dx.doi.org/10.1016/j.etp.2011.01.014] [PMID: 21371873]
[39]
Nigam M, Mishra AP, Adhikari-Devkota A, et al. Fruits of Terminalia chebula Retz.: A review on traditional uses, bioactive chemical constituents and pharmacological activities. Phytother Res 2020; 34(10): 2518-33.
[http://dx.doi.org/10.1002/ptr.6702] [PMID: 32307775]
[40]
Wei X, Luo C, He Y, Huang H, Ran F, Liao W. Hepatoprotective effects of different extracts from triphala against CCl 4 -induced acute liver injury in mice. Front Pharmacol 2021; 12: 1-18.
[41]
Basha SJ, Reddy VJ, Rani YS, Koshma M, Hanumanthu G. A review on terminalia Chebula. Int J of Pharmc Res 2017; 7(10): 187-91.
[42]
Dharmaratne MP, Manoraj A, Thevanesam V. Terminalia bellirica fruit extracts_ in-vitro antibacterial activity against selected multidrug-resistant bacteria, radical scavenging activity and cytotoxicity Study on BHK-21 Cells. BMC Complement Med Ther 2018; 18: 325.
[43]
Choi MK, Kim HG, Han JM, et al. Hepatoprotective effect of terminalia chebula against T-BHP-induced acute liver injury in C57/BL6 mice. Evidence-based Complement Altern Med 2015; 2015: 517350.
[44]
Li N, Li B, Zhang J, et al. Protective effect of phenolic acids from chebulae fructus immaturus on carbon tetrachloride induced acute liver injury via suppressing oxidative stress, inflammation and apoptosis in mouse protective effect of phenolic acids from Chebulae fructus immatur. Nat Prod Res 2019; 34(22): 3249-52.
[45]
Zhang Y, Zhao L, Guo X, et al. Chemical constituents from Phyllanthus emblica and the cytoprotective effects on H2O2-induced PC12 cell injuries. Arch Pharm Res 2016; 39(9): 1202-11.
[http://dx.doi.org/10.1007/s12272-014-0433-2] [PMID: 24993870]
[46]
Thilakchand KR, Mathai RT, Simon P, Ravi RT, Baliga-Rao MP, Baliga MS. Hepatoprotective properties of the Indian gooseberry (Emblica officinalis Gaertn): A review. Food Funct 2013; 4(10): 1431-41.
[http://dx.doi.org/10.1039/c3fo60237k] [PMID: 23978895]
[47]
Yin K, Li X, Luo X, et al. Hepatoprotective effect and potential mechanism of aqueous extract from Phyllanthus emblica on carbontetrachloride-induced liver fibrosis in rats. Evidence-based Complement Altern Med 2021; 2021: 5345821.
[48]
Diab KA, Ibrahim NE, Fahmy MA, Hassan EM, Omara EA. Inhibitory activity of flaxseed oil against CdCl2 induced liver and kidney damage: Histopathology, genotoxicity, and gene expression study. Toxicol Rep 2020; 7: 1127-37.
[http://dx.doi.org/10.1016/j.toxrep.2020.08.023] [PMID: 32963966]
[49]
Kamel EO, Gad-Elrab WM, Ahmed MA, Mohammedsaleh ZM, Hassanein EHM, Ali FEM. Candesartan protects against cadmiuminduced hepatorenal syndrome by affecting Nrf2, NF-κB, Bax/Bcl-2/Cyt-C, and Ang II/Ang 1-7 signals. Biol Trace Elem Res 2022. Epub ahead of print
[http://dx.doi.org/10.1007/s12011-022-03286-4]
[50]
Sepehrinezhad A, Shahbazi A, Sahab Negah S, Joghataei MT, Larsen FS. Drug-induced-acute liver failure: A critical appraisal of the thio-acetamide model for the study of hepatic encephalopathy. Toxicol Rep 2021; 8: 962-70.
[http://dx.doi.org/10.1016/j.toxrep.2021.04.011] [PMID: 34026559]
[51]
Shirin H, Sharvit E, Aeed H, Gavish D, Bruck R. Atorvastatin and rosuvastatin do not prevent thioacetamide induced liver cirrhosis in rats. World J Gastroenterol 2013; 19(2): 241-8.
[http://dx.doi.org/10.3748/wjg.v19.i2.241] [PMID: 23345947]
[52]
Hoffmann K, Nagel AJ, Tanabe K, et al. Markers of liver regeneration-the role of growth factors and cytokines: A systematic review. BMC Surg 2020; 20(1): 31.
[http://dx.doi.org/10.1186/s12893-019-0664-8] [PMID: 32050952]
[53]
Fontes-Cal TCM, Mattos RT, Medeiros NI, et al. Crosstalk between plasma cytokines, inflammation, and liver damage as a new strategy to monitoring NAFLD progression. Front Immunol 2021; 12: 708959.
[http://dx.doi.org/10.3389/fimmu.2021.708959] [PMID: 34447378]

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