Abstract
A novel osteoprotegerin (OPG) isoform cDNA was cloned and expressed in the insect cell and yeast. No homodimeric OPG was found according to the SDS-PAGE analysis and western blot. The OPG isoform was 8 amino acid residues less than the reported hOPG. Genomic DNA of Chinese PBL and HEK 293 cells sequence analysis revealed that the mutation was not the result of RNA editing. The rhOPG with His-Tag at N terminal did not express the hypocalcemic effect in the normal mice.
Protein & Peptide Letters
Title:A Novel Isoform of Osteoprotegerin Gene: Cloning and Expression and Its Hypocalcemic Effect in Mice
Volume: 7 Issue: 4
Author(s): Zhi-Yong He, Guan-Zhen Yang, Zhe-Yu Chen, Biao Li, Wei-Jie Zhang and Xiang-Fu Wu
Affiliation:
Abstract: A novel osteoprotegerin (OPG) isoform cDNA was cloned and expressed in the insect cell and yeast. No homodimeric OPG was found according to the SDS-PAGE analysis and western blot. The OPG isoform was 8 amino acid residues less than the reported hOPG. Genomic DNA of Chinese PBL and HEK 293 cells sequence analysis revealed that the mutation was not the result of RNA editing. The rhOPG with His-Tag at N terminal did not express the hypocalcemic effect in the normal mice.
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Cite this article as:
He Zhi-Yong, Yang Guan-Zhen, Chen Zhe-Yu, Li Biao, Zhang Wei-Jie and Wu Xiang-Fu, A Novel Isoform of Osteoprotegerin Gene: Cloning and Expression and Its Hypocalcemic Effect in Mice, Protein & Peptide Letters 2000; 7 (4) . https://dx.doi.org/10.2174/092986650704221206163712
DOI https://dx.doi.org/10.2174/092986650704221206163712 |
Print ISSN 0929-8665 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5305 |
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