Long-Short-Long Games in mRNA Identification: The Length Matters

San   Ming   Wang

doi:10.2174/138920108785915166

Abstract

The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.

Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome

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Current Pharmaceutical Biotechnology

Title: Long-Short-Long Games in mRNA Identification: The Length Matters

Volume: 9 Issue: 5

Author(s): San Ming Wang

Affiliation:

Keywords: Full-length cDNA, EST, SAGE, LongSAGE, SuperSAGE, transcriptome

Abstract: The multiple levels of post-transcriptional processing and million-fold differences in expression levels make fully decoding the transcriptome in any given species extremely challenging. This review addresses the influence of sequenced length on transcriptome decoding under current DNA sequencing capabilities. Comparison of full-length cDNA, EST, 14-base SAGE, 21-LongSAGE, 26-SuperSAGE, and other variations shows that the sequenced length has been a key factor in determining the sensitivity and specificity for mRNA detection.

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Wang Ming San, Long-Short-Long Games in mRNA Identification: The Length Matters, Current Pharmaceutical Biotechnology 2008; 9 (5) . https://dx.doi.org/10.2174/138920108785915166