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Protein & Peptide Letters

Editor-in-Chief

ISSN (Print): 0929-8665
ISSN (Online): 1875-5305

Research Article

Amplification of Amyloid Protein-induced Aggregation of the Eukaryotic Ribosome

Author(s): Sehnaz Ferdosh, Senjuti Banerjee and Chandana Barat*

Volume 29, Issue 11, 2022

Published on: 12 October, 2022

Page: [993 - 1005] Pages: 13

DOI: 10.2174/0929866529666220905112156

Price: $65

Abstract

Background: Alzheimer’s disease (AD) is characterized by the aggregation of Tau protein and Amyloid-β peptides (Aβ 1-40 and Aβ 1-42). A loss of ribosomal population is also observed in the neurons in affected regions of AD. Our studies demonstrated that in vitro aggregation of amyloid forming proteins, Aβ peptides and Tau protein variants (AFPs), in the vicinity of yeast 80S ribosome can induce co-aggregation of ribosomal components.

Objective: In this study, the ability of minute quantities of AFP-ribosome co-aggregates to seed the aggregation of a large excess of untreated 80S ribosomes was explored.

Methods: The AFPs were purified using ion-exchange chromatography. Seeded aggregation of ribosomes in the presence of minute quantities of ribosome-protein co-aggregates or ribosomal components was studied using agarose gel electrophoretic and SDS-PAGE analysis of the pellets and Sucrose Density Gradient centrifugation of the supernatant obtained after centrifugation of the aggregation reaction mixture.

Results: Our studies, therefore, demonstrate that minute quantities of AFP-80S co-aggregate have significant seeding potential and could lead to aggregation of a large excess of fresh 80S ribosomes and this seeding ability is sustained over multiple cycles of ribosome aggregation. The aggregation propensity of ribosomal components alone could contribute towards the seeding of ribosome aggregation.

Conclusion: The ability of minute quantities of AFP-80S co-aggregates to seed the aggregation of a large excess of fresh 80S ribosomes would result in the loss of global ribosomal population in Alzheimer’s disease afflicted neurons. Hence, subject to further validation by in vivo studies, our in vitro studies indicate a significant mode of toxicity of amyloid aggregates that might be important in Alzheimer’s disease pathology.

Keywords: Amyloid-β, Tau protein, Ribosome, Aggregation, Seeding, Alzheimer’s disease, Ribosomal RNA (rRNA), Ribosomal protein

Graphical Abstract

[1]
Drew, L. An age-old story of dementia. Nature, 2018, 559(7715), S2-S3.
[http://dx.doi.org/10.1038/d41586-018-05718-5] [PMID: 30046085]
[2]
Cuello, A.C. Early and late CNS inflammation in Alzheimer’s disease: Two extremes of a continuum? Trends Pharmacol. Sci., 2017, 38(11), 956-966.
[http://dx.doi.org/10.1016/j.tips.2017.07.005] [PMID: 28867259]
[3]
Ballatore, C.; Lee, V.M.Y.; Trojanowski, J.Q. Tau-mediated neurodegeneration in Alzheimer’s disease and related disorders. Nat. Rev. Neurosci., 2007, 8(9), 663-672.
[http://dx.doi.org/10.1038/nrn2194] [PMID: 17684513]
[4]
Lee, S.J.C.; Nam, E.; Lee, H.J.; Savelieff, M.G.; Lim, M.H. Towards an understanding of amyloid-β oligomers: Characterization, toxicity mechanisms, and inhibitors. Chem. Soc. Rev., 2017, 46(2), 310-323.
[http://dx.doi.org/10.1039/C6CS00731G] [PMID: 27878186]
[5]
LaFerla, F.M.; Green, K.N.; Oddo, S. Intracellular amyloid-β in Alzheimer’s disease. Nat. Rev. Neurosci., 2007, 8(7), 499-509.
[http://dx.doi.org/10.1038/nrn2168] [PMID: 17551515]
[6]
Ding, Q.; Markesbery, W.R.; Cecarini, V.; Keller, J.N. Decreased RNA, and increased RNA oxidation, in ribosomes from early Alzheimer’s disease. Neurochem. Res., 2006, 31(5), 705-710.
[http://dx.doi.org/10.1007/s11064-006-9071-5] [PMID: 16770743]
[7]
Banerjee, S.; Ferdosh, S.; Ghosh, A.N.; Barat, C. Tau protein-induced sequestration of the eukaryotic ribosome: Implications in neurodegenerative disease. Sci. Rep., 2020, 10(1), 5225.
[http://dx.doi.org/10.1038/s41598-020-61777-7] [PMID: 32251304]
[8]
Ferdosh, S.; Banerjee, S.; Singh, J.; Barat, C. Amyloid protein‐induced sequestration of the eukaryotic ribosome: Effect of stoichiometry and polyphenolic inhibitors. FEBS Lett., 2022, 596(9), 1190-1202.
[http://dx.doi.org/10.1002/1873-3468.14308] [PMID: 35114013]
[9]
Chakraborty, B.; Bhakta, S.; Sengupta, J. Disassembly of yeast 80S ribosomes into subunits is a concerted action of ribosome-assisted folding of denatured protein. Biochem. Biophys. Res. Commun., 2016, 469(4), 923-929.
[http://dx.doi.org/10.1016/j.bbrc.2015.12.107] [PMID: 26723252]
[10]
Walsh, D.M.; Thulin, E.; Minogue, A.M.; Gustavsson, N.; Pang, E.; Teplow, D.B.; Linse, S. A facile method for expression and purification of the Alzheimer’s disease-associated amyloid β-peptide. FEBS J., 2009, 276(5), 1266-1281.
[http://dx.doi.org/10.1111/j.1742-4658.2008.06862.x] [PMID: 19175671]
[11]
Ramachandran, G.; Udgaonkar, J.B. Understanding the kinetic roles of the inducer heparin and of rod-like protofibrils during amyloid fibril formation by Tau protein. J. Biol. Chem., 2011, 286(45), 38948-38959.
[http://dx.doi.org/10.1074/jbc.M111.271874] [PMID: 21931162]
[12]
Ge, J.F.; Qiao, J.P.; Qi, C.C.; Wang, C.W.; Zhou, J.N. The binding of resveratrol to monomer and fibril amyloid beta. Neurochem. Int., 2012, 61(7), 1192-1201.
[http://dx.doi.org/10.1016/j.neuint.2012.08.012] [PMID: 22981725]
[13]
Ono, K.; Li, L.; Takamura, Y.; Yoshiike, Y.; Zhu, L.; Han, F.; Mao, X.; Ikeda, T.; Takasaki, J.; Nishijo, H.; Takashima, A.; Teplow, D.B.; Zagorski, M.G.; Yamada, M. Phenolic compounds prevent amyloid β-protein oligomerization and synaptic dysfunction by site-specific binding. J. Biol. Chem., 2012, 287(18), 14631-14643.
[http://dx.doi.org/10.1074/jbc.M111.325456] [PMID: 22393064]
[14]
Piir, K.; Tamm, T.; Kisly, I.; Tammsalu, T.; Remme, J. Stepwise splitting of ribosomal proteins from yeast ribosomes by LiCl. PLoS One, 2014, 9(7), e101561.
[http://dx.doi.org/10.1371/journal.pone.0101561] [PMID: 24991888]
[15]
Schieber, G.L.; O’Brien, T.W. Extraction of proteins from the large subunit of bovine mitochondrial ribosomes under nondenaturing conditions. J. Biol. Chem., 1982, 257(15), 8781-8787.
[http://dx.doi.org/10.1016/S0021-9258(18)34198-X] [PMID: 6284743]
[16]
Conchillo, S.O.; De Groot, N.S.; Avilés, F.X.; Vendrell, J.; Daura, X.; Ventura, S. AGGRESCAN: A server for the prediction and evaluation of “hot spots” of aggregation in polypeptides. BMC Bioinformatics, 2007, 8(1), 65.
[http://dx.doi.org/10.1186/1471-2105-8-65] [PMID: 17324296]
[17]
Garbuzynskiy, S.O.; Lobanov, M.Y.; Galzitskaya, O.V. FoldAmyloid: A method of prediction of amyloidogenic regions from protein sequence. Bioinformatics, 2010, 26(3), 326-332.
[http://dx.doi.org/10.1093/bioinformatics/btp691] [PMID: 20019059]
[18]
Oliveberg, M. Waltz, an exciting new move in amyloid prediction. Nat. Methods, 2010, 7(3), 187-188.
[http://dx.doi.org/10.1038/nmeth0310-187] [PMID: 20195250]
[19]
Walsh, I.; Seno, F.; Tosatto, S.C.E.; Trovato, A. PASTA 2.0: An improved server for protein aggregation prediction. Nucleic Acids Res., 2014, 42(W1), W301-W307.
[http://dx.doi.org/10.1093/nar/gku399] [PMID: 24848016]
[20]
Grishin, S.Y.; Deryusheva, E.I.; Machulin, A.V.; Selivanova, O.M.; Glyakina, A.V.; Gorbunova, E.Y.; Mustaeva, L.G.; Azev, V.N.; Rekstina, V.V.; Kalebina, T.S.; Surin, A.K.; Galzitskaya, O.V. Amyloidogenic propensities of ribosomal s1 proteins: Bioinformatics screening and experimental checking. Int. J. Mol. Sci., 2020, 21(15), 5199.
[http://dx.doi.org/10.3390/ijms21155199] [PMID: 32707977]
[21]
Friesen, M. Meyer, L.M. Aβ seeding as a tool to study cerebral amyloidosis and associated pathology. Front. Mol. Neurosci., 2019, 12, 233.
[http://dx.doi.org/10.3389/fnmol.2019.00233] [PMID: 31632238]
[22]
Makarava, N.; Savtchenko, R.; Baskakov, I.V. Methods of protein misfolding cyclic amplification. Methods Mol. Biol., 2017, 1658, 169-183.
[http://dx.doi.org/10.1007/978-1-4939-7244-9_13] [PMID: 28861790]
[23]
Meyer, V.; Dinkel, P.D.; Rickman Hager, E.; Margittai, M. Amplification of Tau fibrils from minute quantities of seeds. Biochemistry, 2014, 53(36), 5804-5809.
[http://dx.doi.org/10.1021/bi501050g] [PMID: 25153692]
[24]
Tran, J.; Chang, D.; Hsu, F.; Wang, H.; Guo, Z. Cross-seeding between Aβ40 and Aβ42 in Alzheimer’s disease. FEBS Lett., 2017, 591(1), 177-185.
[http://dx.doi.org/10.1002/1873-3468.12526] [PMID: 27981583]
[25]
Dinkel, P.D.; Holden, M.R.; Matin, N.; Margittai, M. RNA binds to tau fibrils and sustains template-assisted growth. Biochemistry, 2015, 54(30), 4731-4740.
[http://dx.doi.org/10.1021/acs.biochem.5b00453] [PMID: 26177386]
[26]
Harper, J.D.; Lansbury, P.T., Jr Models of amyloid seeding in Alzheimer’s disease and scrapie: Mechanistic truths and physiological consequences of the time-dependent solubility of amyloid proteins. Annu. Rev. Biochem., 1997, 66(1), 385-407.
[http://dx.doi.org/10.1146/annurev.biochem.66.1.385] [PMID: 9242912]
[27]
Jäkel, S.; Mingot, J.M.; Schwarzmaier, P.; Hartmann, E.; Görlich, D. Importins fulfil a dual function as nuclear import receptors and cytoplasmic chaperones for exposed basic domains. EMBO J., 2002, 21(3), 377-386.
[http://dx.doi.org/10.1093/emboj/21.3.377] [PMID: 11823430]
[28]
Koch, B.; Mitterer, V.; Niederhauser, J.; Stanborough, T.; Murat, G.; Rechberger, G.; Bergler, H.; Kressler, D.; Pertschy, B. Yar1 protects the ribosomal protein Rps3 from aggregation. J. Biol. Chem., 2012, 287(26), 21806-21815.
[http://dx.doi.org/10.1074/jbc.M112.365791] [PMID: 22570489]
[29]
Pillet, B.; García, G.J.J.; Pausch, P.; Falquet, L.; Bange, G.; De La, C.J.; Kressler, D. The dedicated chaperone Acl4 escorts ribosomal protein Rpl4 to its nuclear pre-60S assembly site. PLoS Genet., 2015, 11(10), e1005565.
[http://dx.doi.org/10.1371/journal.pgen.1005565] [PMID: 26447800]
[30]
Peng, Z.; Oldfield, C.J.; Xue, B.; Mizianty, M.J.; Dunker, A.K.; Kurgan, L.; Uversky, V.N. A creature with a hundred waggly tails: Intrinsically disordered proteins in the ribosome. Cell. Mol. Life Sci., 2014, 71(8), 1477-1504.
[http://dx.doi.org/10.1007/s00018-013-1446-6] [PMID: 23942625]
[31]
Timsit, Y.; Acosta, Z.; Allemand, F.; Chiaruttini, C.; Springer, M. The role of disordered ribosomal protein extensions in the early steps of eubacterial 50 S ribosomal subunit assembly. Int. J. Mol. Sci., 2009, 10(3), 817-834.
[http://dx.doi.org/10.3390/ijms10030817] [PMID: 19399222]
[32]
Uversky, V. Amyloidogenesis of natively unfolded proteins. Curr. Alzheimer Res., 2008, 5(3), 260-287.
[http://dx.doi.org/10.2174/156720508784533312] [PMID: 18537543]
[33]
Gunawardana, C.G.; Mehrabian, M.; Wang, X.; Mueller, I.; Lubambo, I.B.; Jonkman, J.E.N.; Wang, H.; Schmitt-Ulms, G. The human tau interactome: Binding to the ribonucleoproteome, and impaired binding of the proline-to-leucine mutant at Position 301 (P301L) to chaperones and the proteasome. Mol. Cell. Proteomics, 2015, 14(11), 3000-3014.
[http://dx.doi.org/10.1074/mcp.M115.050724] [PMID: 26269332]
[34]
Meier, S.; Bell, M.; Lyons, D.N.; Rodriguez, R.J.; Ingram, A.; Fontaine, S.N.; Mechas, E.; Chen, J.; Wolozin, B.; LeVine, H., III; Zhu, H.; Abisambra, J.F. Pathological tau promotes neuronal damage by impairing ribosomal function and decreasing protein synthesis. J. Neurosci., 2016, 36(3), 1001-1007.
[http://dx.doi.org/10.1523/JNEUROSCI.3029-15.2016] [PMID: 26791227]
[35]
Iseki, E.; Matsumura, T.; Marui, W.; Hino, H.; Odawara, T.; Sugiyama, N.; Suzuki, K.; Sawada, H.; Arai, T.; Kosaka, K. Familial frontotemporal dementia and parkinsonism with a novel N296H mutation in exon 10 of the tau gene and a widespread tau accumulation in the glial cells. Acta Neuropathol., 2001, 102(3), 285-292.
[http://dx.doi.org/10.1007/s004010000333] [PMID: 11585254]
[36]
Nelson, P.T.; Marton, L.; Saper, C.B. Alz-50 immunohisto-chemistry in the normal sheep striatum: A light and electron microscope study. Brain Res., 1993, 600(2), 285-297.
[http://dx.doi.org/10.1016/0006-8993(93)91385-6] [PMID: 8094642]
[37]
Nelson, P.T.; Saper, C.B. Ultrastructure of neurofibrillary tangles in the cerebral cortex of sheep. Neurobiol. Aging, 1995, 16(3), 315-323.
[http://dx.doi.org/10.1016/0197-4580(94)00175-Z] [PMID: 7566341]
[38]
Papasozomenos, S.C. Tau protein immunoreactivity in dementia of the Alzheimer type. I. Morphology, evolution, distribution, and pathogenetic implications. Lab. Invest., 1989, 60(1), 123-137.
[PMID: 2492060]
[39]
Papasozomenos, S.C.; Binder, L.I. Phosphorylation determines two distinct species of tau in the central nervous system. Cell Motil. Cytoskeleton, 1987, 8(3), 210-226.
[http://dx.doi.org/10.1002/cm.970080303] [PMID: 2446784]
[40]
Piao, Y.S.; Hayashi, S.; Wakabayashi, K.; Kakita, A.; Aida, I.; Yamada, M.; Takahashi, H. Cerebellar cortical tau pathology in progressive supranuclear palsy and corticobasal degeneration. Acta Neuropathol., 2002, 103(5), 469-474.
[http://dx.doi.org/10.1007/s00401-001-0488-2] [PMID: 11935262]
[41]
Tanemura, K.; Murayama, M.; Akagi, T.; Hashikawa, T.; Tominaga, T.; Ichikawa, M.; Yamaguchi, H.; Takashima, A. Neurodegeneration with tau accumulation in a transgenic mouse expressing V337M human tau. J. Neurosci., 2002, 22(1), 133-141.
[http://dx.doi.org/10.1523/JNEUROSCI.22-01-00133.2002] [PMID: 11756496]
[42]
Koren, S.A.; Hamm, M.J.; Meier, S.E.; Weiss, B.E.; Nation, G.K.; Chishti, E.A.; Arango, J.P.; Chen, J.; Zhu, H.; Blalock, E.M.; Abisambra, J.F. Tau drives translational selectivity by interacting with ribosomal proteins. Acta Neuropathol., 2019, 137(4), 571-583.
[http://dx.doi.org/10.1007/s00401-019-01970-9] [PMID: 30759285]
[43]
Ding, Q.; Markesbery, W.R.; Chen, Q.; Li, F.; Keller, J.N. Ribosome dysfunction is an early event in Alzheimer’s disease. J. Neurosci., 2005, 25(40), 9171-9175.
[http://dx.doi.org/10.1523/JNEUROSCI.3040-05.2005] [PMID: 16207876]
[44]
Langstrom, N.S.; Anderson, J.P.; Lindroos, H.G.; Winbland, B.; Wallace, W.C. Alzheimer’s disease-associated reduction of polysomal mRNA translation. Brain Res. Mol. Brain Res., 1989, 5(4), 259-269.
[http://dx.doi.org/10.1016/0169-328X(89)90060-0] [PMID: 2747450]
[45]
Mann, D.M.; Neary, D.; Yates, P.O.; Lincoln, J.; Snowden, J.S.; Stanworth, P. Alterations in protein synthetic capability of nerve cells in Alzheimer’s disease. J. Neurol. Neurosurg. Psychiatry, 1981, 44(2), 97-102.
[http://dx.doi.org/10.1136/jnnp.44.2.97] [PMID: 6163842]
[46]
Sajdel, S.E.M.; Marotta, C.A. Alzheimer’s disease brain: Alterations in RNA levels and in a ribonuclease-inhibitor complex. Science, 1984, 225(4665), 947-949.
[http://dx.doi.org/10.1126/science.6206567] [PMID: 6206567]
[47]
Ginsberg, S.D.; Crino, P.B.; Lee, V.M.Y.; Eberwine, J.H.; Trojanowski, J.Q. Sequestration of RNA in Alzheimer’s disease neurofibrillary tangles and senile plaques. Ann. Neurol., 1997, 41(2), 200-209.
[http://dx.doi.org/10.1002/ana.410410211] [PMID: 9029069]
[48]
Ginsberg, S.D.; Galvin, J.E.; Chiu, T.S.; Lee, V.M.Y.; Masliah, E.; Trojanowski, J.Q. RNA sequestration to pathological lesions of neurodegenerative diseases. Acta Neuropathol., 1998, 96(5), 487-494.
[http://dx.doi.org/10.1007/s004010050923] [PMID: 9829812]
[49]
Yang, H.; Hu, H.Y. Sequestration of cellular interacting partners by protein aggregates: Implication in a loss‐of‐function pathology. FEBS J., 2016, 283(20), 3705-3717.
[http://dx.doi.org/10.1111/febs.13722] [PMID: 27016044]
[50]
Chakraborty, P.; Rivière, G.; Liu, S.; De Opakua, A.I. Dervişoğlu, R; Hebestreit, A; Andreas, LB; Vorberg, IM; Zweckstetter, M Co-factor-free aggregation of tau into seeding-competent RNA-sequestering amyloid fibrils. Nature Communications, 2021, 12(1), 4231.
[http://dx.doi.org/10.1038/s41467-021-24362-8] [PMID: 34244499]
[51]
Brunello, C.A.; Merezhko, M.; Uronen, R.L.; Huttunen, H.J. Mechanisms of secretion and spreading of pathological tau protein. Cell. Mol. Life Sci., 2020, 77(9), 1721-1744.
[http://dx.doi.org/10.1007/s00018-019-03349-1] [PMID: 31667556]
[52]
Clavaguera, F.; Hench, J.; Goedert, M.; Tolnay, M. Invited review: Prion-like transmission and spreading of tau pathology. Neuropathol. Appl. Neurobiol., 2015, 41(1), 47-58.
[http://dx.doi.org/10.1111/nan.12197] [PMID: 25399729]

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    • (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_journal_volume_trail WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_journal_volume_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_journal_volume_token WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_ebook_volume_trail WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_ebook_volume_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_ebook_volume_token WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_issue_trail WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_issue_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_issue_token WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_article_trail WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_article_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_article_token WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_chapter_trail WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_chapter_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_chapter_token WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) union (select `user_id`, `to_ip`, `from_ip` from (select user_id, to_ip, from_ip FROM user_access_disease_corporate WHERE to_ip >= 873421719 ORDER BY to_ip ASC LIMIT 1) as `tjv` where `from_ip` <= 873421719) limit 1
      18.95msalphaeurekaselec_live_10_06_2022UserAccess.php#784
      Bindings
      • 0: 873421719
      • 1: 873421719
      • 2: 873421719
      • 3: 873421719
      • 4: 873421719
      • 5: 873421719
      • 6: 873421719
      • 7: 873421719
      • 8: 873421719
      • 9: 873421719
      • 10: 873421719
      • 11: 873421719
      • 12: 873421719
      • 13: 873421719
      • 14: 873421719
      • 15: 873421719
      Backtrace
      • 14. app/Models/UserAccess/UserAccess.php:784
      • 15. middleware::check_user_access:114
      • 16. vendor/laravel/framework/src/Illuminate/Pipeline/Pipeline.php:183
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Middleware/SubstituteBindings.php:50
      • 18. vendor/laravel/framework/src/Illuminate/Pipeline/Pipeline.php:183
    • SELECT at.type_name, p.copyright, p.publisher_location,m.month,y.year, a.publish_on, a.nid article_nid,a.article_id, a.pmid, a.issue_id, a.title, a.abstract,a.podcast,a.altmetric_score,a.altmetric_url,a.kudos_url, a.page_count, a.first_page, a.elocator, a.last_page, i.publication_date, a.file_name, a.file_path, a.doi, a.is_epub,a.epub_type, a.volume_id, a.is_uploaded, a.editor_choice, a.is_epub, j.image_file_name, a.article_no, a.art_type AS article_type, a.supplementary_filename,a.html_filename,a.prc_filename,a.epub_filename, a.graph_abs_lg_filename, a.graph_abs_sm_filename, a.oa_image_lg_filename, a.oa_image_sm_filename, j.journal_banner,a.animated_abstract,a.erratum_in,a.erratum_for, (CASE WHEN a.issue_id =0 THEN j.epub_price ELSE j.price END) AS sell_price, j.epub_price, j.nid AS jour_nid, j.title AS journal_title, j.issn, j.eissn,j.journal_id, j.subtitle AS jour_subtitle, v.volume_id,v.volume_name AS volume, v.year_id, i.title AS issue, i.month_id ,i.type issue_type, j.subtitle,j.formerly_title, ac.subtitle AS epub_title,a.crossmark_enabled,a.text_mining_urls,a.license_urls,a.created, a.receivedate, a.revisedate, a.acceptdate,js.js_title FROM article a LEFT JOIN article_section ac ON a.is_epub = ac.art_sec_id LEFT JOIN journal_section js ON a.js_id = js.js_id LEFT JOIN article_type at ON a.art_type = at.art_type_id LEFT JOIN issue i ON a.issue_id = i.issue_id LEFT JOIN month m ON m.id = i.month_id LEFT JOIN volume v ON v.volume_id = a.volume_id INNER JOIN year y ON y.id = v.year_id INNER JOIN journal j ON j.journal_id = a.journal_id INNER JOIN publisher p ON p.publisher_id = j.publisher_id WHERE a.article_id=126152
      990μsalphaeurekaselec_live_10_06_2022Article.php#408
      Backtrace
      • 11. app/Models/Article.php:408
      • 12. app/Http/Controllers/ArticleController.php:2515
      • 13. app/Http/Controllers/ArticleController.php:2035
      • 14. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 15. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select * from `journal` where `journal_id` = 51 limit 1
      980μsalphaeurekaselec_live_10_06_2022Journal.php#64
      Bindings
      • 0: 51
      Backtrace
      • 14. app/Models/Journal.php:64
      • 15. app/Http/Controllers/ArticleController.php:2516
      • 16. app/Http/Controllers/ArticleController.php:2035
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 18. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select count(*) as aggregate from (select j.nid as journal_nid,j.flyer_link,j.title ,j.issn,j.eissn,j.keyword, j.journal_id as journal_id, j.subtitle, j.image_file_name,j.description,j.journal_insight_url,j.doi from `subject_journal` as `sj` inner join `journal` as `j` on `j`.`journal_id` = `sj`.`journal_id` where j.journal_status=1 and subject_id IN (SELECT subject_id FROM subject_journal WHERE journal_id=51) and sj.journal_id!=51 group by `j`.`nid`) as `aggregate_table`
      1.11msalphaeurekaselec_live_10_06_2022Subject.php#200
      Backtrace
      • 16. app/Models/Subject.php:200
      • 17. app/Http/Controllers/ArticleController.php:2517
      • 18. app/Http/Controllers/ArticleController.php:2035
      • 19. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 20. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select j.nid as journal_nid,j.flyer_link,j.title ,j.issn,j.eissn,j.keyword, j.journal_id as journal_id, j.subtitle, j.image_file_name,j.description,j.journal_insight_url,j.doi from `subject_journal` as `sj` inner join `journal` as `j` on `j`.`journal_id` = `sj`.`journal_id` where j.journal_status=1 and subject_id IN (SELECT subject_id FROM subject_journal WHERE journal_id=51) and sj.journal_id!=51 group by `j`.`nid` order by `j`.`journal_id` asc limit 10 offset 0
      1msalphaeurekaselec_live_10_06_2022Subject.php#200
      Backtrace
      • 14. app/Models/Subject.php:200
      • 15. app/Http/Controllers/ArticleController.php:2517
      • 16. app/Http/Controllers/ArticleController.php:2035
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 18. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select count(*) as aggregate from (select v.nid as volume_id,e.nid,e.title,v.issn,v.eissn,v.isbn,v.eisbn,e.ebook_id, v.image_file_name,v.file_path,v.flyer_link,v.ebook_volume_id, v.volume_name,v.doi,v.year_id,v.introduction from `subject_ebook` as `se` inner join `ebook` as `e` on `e`.`ebook_id` = `se`.`ebook_id` inner join `ebook_volume` as `v` on `v`.`ebook_id` = `se`.`ebook_id` inner join `year` as `y` on `y`.`id` = `v`.`year_id` where subject_id IN (SELECT subject_id FROM subject_journal WHERE journal_id=51) and v.ebook_status='1' and y.year > 2020 group by `v`.`nid`) as `aggregate_table`
      5.7msalphaeurekaselec_live_10_06_2022Subject.php#220
      Backtrace
      • 16. app/Models/Subject.php:220
      • 17. app/Http/Controllers/ArticleController.php:2518
      • 18. app/Http/Controllers/ArticleController.php:2035
      • 19. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 20. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select v.nid as volume_id,e.nid,e.title,v.issn,v.eissn,v.isbn,v.eisbn,e.ebook_id, v.image_file_name,v.file_path,v.flyer_link,v.ebook_volume_id, v.volume_name,v.doi,v.year_id,v.introduction from `subject_ebook` as `se` inner join `ebook` as `e` on `e`.`ebook_id` = `se`.`ebook_id` inner join `ebook_volume` as `v` on `v`.`ebook_id` = `se`.`ebook_id` inner join `year` as `y` on `y`.`id` = `v`.`year_id` where subject_id IN (SELECT subject_id FROM subject_journal WHERE journal_id=51) and v.ebook_status='1' and y.year > 2020 group by `v`.`nid` order by `v`.`ebook_volume_id` desc limit 10 offset 0
      5.01msalphaeurekaselec_live_10_06_2022Subject.php#220
      Backtrace
      • 14. app/Models/Subject.php:220
      • 15. app/Http/Controllers/ArticleController.php:2518
      • 16. app/Http/Controllers/ArticleController.php:2035
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 18. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select * from `keywords` where `article_id` = '126152'
      1.3msalphaeurekaselec_live_10_06_2022Keywords.php#43
      Bindings
      • 0: 126152
      Backtrace
      • 13. app/Models/Keywords.php:43
      • 14. app/Http/Controllers/ArticleController.php:2519
      • 15. app/Http/Controllers/ArticleController.php:2035
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select * from `article_citation` where `article_id` = '126152'
      4.39msalphaeurekaselec_live_10_06_2022Article.php#1570
      Bindings
      • 0: 126152
      Backtrace
      • 13. app/Models/Article.php:1570
      • 14. app/Http/Controllers/ArticleController.php:2520
      • 15. app/Http/Controllers/ArticleController.php:2035
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select CONCAT_WS(' ',au.first_name, ' ', ifnull(au.initials,''), ' ', au.last_name) as authors, CONCAT(au.last_name, IFNULL(CONCAT(' ', au.initials), ''), ' ', au.first_name) AS authorsRIS, CONCAT_WS(' ',au.last_name, ' ', ifnull(au.initials,''), ' ', au.first_name) as authorsCiteAs, CONCAT_WS(' ',au.first_name, IFNULL(CONCAT(' ', au.initials), ''), IFNULL(CONCAT(' ', au.last_name), '')) as authorsmodal , `au`.*, `af`.`ror_id`, `af`.`institution`, `af`.`department`, `af`.`country`, `af`.`city`, `af`.`address`, (GROUP_CONCAT(TRIM(BOTH ', ' FROM CONCAT(ifnull(concat(af.institution,','),''), ifnull(concat(af.department,','),''), ifnull(concat(af.address,','),''), ifnull(concat(af.city,','),''), ifnull(concat(af.country,','),''), ifnull(concat(af.postal_code,','),''), ifnull(concat(af.phone,','),''), ifnull(concat(af.fax,','),''))) SEPARATOR '|')) as `author_affiliation`, (GROUP_CONCAT(TRIM(BOTH ', ' FROM CONCAT(ifnull(concat(af.web_view,','),''))) SEPARATOR '|')) as `web_view` from `author` as `au` left join `author_affiliation` as `af` on `au`.`author_id` = `af`.`author_id` where `au`.`article_id` = '126152' group by `au`.`author_id` order by `au`.`article_id` asc, `au`.`sequence` asc
      1.8msalphaeurekaselec_live_10_06_2022Author.php#86
      Bindings
      • 0: 126152
      Backtrace
      • 13. app/Models/Author.php:86
      • 14. app/Http/Controllers/ArticleController.php:2533
      • 15. app/Http/Controllers/ArticleController.php:2035
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select `bo`.* from `bundle_offer` as `bo` where `bo`.`bundle_status` = 'A' order by `bo`.`bundle_id` desc
      210μsalphaeurekaselec_live_10_06_2022BundleOffer.php#57
      Bindings
      • 0: A
      Backtrace
      • 13. app/Models/BundleOffer.php:57
      • 14. app/Http/Controllers/ArticleController.php:2535
      • 15. app/Http/Controllers/ArticleController.php:2035
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
    • select `subtitle` from `journal` where `continues_publication_journal` = 1
      470μsalphaeurekaselec_live_10_06_2022Article.php#1960
      Bindings
      • 0: 1
      Backtrace
      • 13. app/Models/Article.php:1960
      • 14. app/Http/Controllers/ArticleController.php:2062
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • Select j.subtitle, v.volume_id as volume_id,v.year_id,y.year as year,v.volume_name, v.total_no_of_issues, i.issue_id as issue_id,i.title as issue, i.publication_date as issue_pub_date from journal j join volume v on j.journal_id = v.journal_id join issue i on v.volume_id = i.volume_id join article a on a.issue_id = i.issue_id join year y on v.year_id = y.id where j.journal_id = 51 and i.is_uploaded = 1 order by v.volume_name+0 desc, i.title+0 desc limit 1
      27.58msalphaeurekaselec_live_10_06_2022Issue.php#298
      Backtrace
      • 11. app/Models/Issue.php:298
      • 12. app/Http/Controllers/ArticleController.php:2068
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select a.nid,a.publish_on, a.pmid,a.article_id,a.doi,j.journal_id,j.subtitle,v.volume_name,a.volume_id as volume_id, a.issue_id,a.title,first_page, last_page, page_count,abstract,ifnull(is_abstract,"y"),asec.title as article_section,at.type_name as article_type_name, a.article_id as ArticleID ,is_epub,epub_price,epub_type,a.created, y.year from `article` as `a` left join `article_section` as `asec` on `asec`.`art_sec_id` = `a`.`is_epub` left join `article_type` as `at` on `at`.`art_type_id` = `a`.`art_type` left join `journal` as `j` on `j`.`journal_id` = `a`.`journal_id` inner join `volume` as `v` on `a`.`volume_id` = `v`.`volume_id` inner join `year` as `y` on `v`.`year_id` = `y`.`id` where a.article_status != "W" and a.is_epub = 1 and a.journal_id = 51 and a.is_uploaded = 1 group by `a`.`article_id` order by `a`.`publish_on` desc
      15.24msalphaeurekaselec_live_10_06_2022Article.php#528
      Backtrace
      • 13. app/Models/Article.php:528
      • 14. app/Http/Controllers/ArticleController.php:2070
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select a.nid,a.publish_on, a.pmid,a.article_id,a.doi,j.journal_id,j.subtitle,v.volume_name,a.volume_id as volume_id, a.issue_id,a.title,first_page, last_page, page_count,abstract,ifnull(is_abstract,"y"),asec.title as article_section,at.type_name as article_type_name, a.article_id as ArticleID ,is_epub,epub_price,epub_type,a.created, y.year from `article` as `a` left join `article_section` as `asec` on `asec`.`art_sec_id` = `a`.`is_epub` left join `article_type` as `at` on `at`.`art_type_id` = `a`.`art_type` left join `journal` as `j` on `j`.`journal_id` = `a`.`journal_id` inner join `volume` as `v` on `a`.`volume_id` = `v`.`volume_id` inner join `year` as `y` on `v`.`year_id` = `y`.`id` where a.article_status != "W" and a.is_epub = 4 and a.journal_id = 51 and a.is_uploaded = 1 group by `a`.`article_id` order by `a`.`publish_on` desc
      13.72msalphaeurekaselec_live_10_06_2022Article.php#528
      Backtrace
      • 13. app/Models/Article.php:528
      • 14. app/Http/Controllers/ArticleController.php:2071
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select pdf, html, epub, prc from `article_metrics` where `article_id` = 126152 limit 1
      830μsalphaeurekaselec_live_10_06_2022Article.php#744
      Bindings
      • 0: 126152
      Backtrace
      • 14. app/Models/Article.php:744
      • 15. app/Http/Controllers/ArticleController.php:2091
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 18. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select * from content_by_diseases where content_id = 126152 and content_type = 'article' order by status desc limit 1
      270μsalphaeurekaselec_live_10_06_2022Article.php#751
      Backtrace
      • 11. app/Models/Article.php:751
      • 12. app/Http/Controllers/ArticleController.php:2094
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select * from `node_meta` where `nid` = 208535 limit 1
      240μsalphaeurekaselec_live_10_06_2022Meta.php#30
      Bindings
      • 0: 208535
      Backtrace
      • 14. app/Models/Meta.php:30
      • 15. app/Http/Controllers/ArticleController.php:2101
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 18. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select `bo`.* from `bundle_offer` as `bo` where `bo`.`bundle_status` = 'A' order by `bo`.`bundle_id` desc
      290μsalphaeurekaselec_live_10_06_2022BundleOffer.php#57
      Bindings
      • 0: A
      Backtrace
      • 13. app/Models/BundleOffer.php:57
      • 14. app/Http/Controllers/ArticleController.php:2108
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select * from `tbl_ht_submission` as `h` where `h`.`subtitle` = 'PPL' and (h.proposal_closing_date = 0 OR TO_DAYS(FROM_UNIXTIME(h.proposal_closing_date)) >= TO_DAYS(NOW())) order by `h`.`manuscript` asc
      310μsalphaeurekaselec_live_10_06_2022Article.php#2025
      Bindings
      • 0: PPL
      Backtrace
      • 13. app/Models/Article.php:2025
      • 14. app/Http/Controllers/ArticleController.php:2112
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select count(*) as total_count, content_type_access_id as access_type from journal_access where content_type_id = '126152' and content_type = 'a' and ( (from_date <= CURDATE() and to_date >= CURDATE() and perpetual= 0) or (from_date is null and to_date is null and perpetual= 1) )
      450μsalphaeurekaselec_live_10_06_2022ContentAccess.php#647
      Backtrace
      • 11. app/Models/ContentAccess.php:647
      • 12. app/Http/Controllers/ArticleController.php:2186
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select count(*) as total_count, content_type_access_id as access_type from journal_access where content_type_id = '11935' and content_type = 'i' and ( (from_date <= CURDATE() and to_date >= CURDATE() and perpetual= 0) or (from_date is null and to_date is null and perpetual= 1) )
      380μsalphaeurekaselec_live_10_06_2022ContentAccess.php#647
      Backtrace
      • 11. app/Models/ContentAccess.php:647
      • 12. app/Http/Controllers/ArticleController.php:2207
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select count(*) as total_count, content_type_access_id as access_type from journal_access where content_type_id = '3176' and content_type = 'v' and ( (from_date <= CURDATE() and to_date >= CURDATE() and perpetual= 0) or (from_date is null and to_date is null and perpetual= 1) )
      370μsalphaeurekaselec_live_10_06_2022ContentAccess.php#647
      Backtrace
      • 11. app/Models/ContentAccess.php:647
      • 12. app/Http/Controllers/ArticleController.php:2231
      • 13. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 14. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"volume" as access_level,"" as issue_id,"" as article_id from `user_access_journal_volume_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.volume_id = 3176) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"volume" as access_level,"" as issue_id,"" as article_id from `user_access_journal_volume_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.volume_id = 3176) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"volume" as access_level,"" as issue_id,"" as article_id from `user_access_journal_volume_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.volume_id = 3176)
      25.8msalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ArticleController.php:2272
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"issue" as access_level,j.issue_id,"" as article_id from `user_access_issue_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.issue_id = 11935) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"issue" as access_level,j.issue_id,"" as article_id from `user_access_issue_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.issue_id = 11935) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"issue" as access_level,j.issue_id,"" as article_id from `user_access_issue_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.issue_id = 11935)
      670μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ArticleController.php:2297
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Trial Access" as access_base_text, CASE j.type WHEN 111 THEN true ELSE false END as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"article" as access_level, j.issue_id, j.article_id from `user_access_article_trail` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.article_id = 126152) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Subscribed" as access_base_text, false as terms_popup, "S" as content_download_type, null as restricted_user_access_key, null as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"article" as access_level, j.issue_id, j.article_id from `user_access_article_corporate` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.article_id = 126152) union (select j.user_access_id,j.volume_id,j.user_id,j.from_ip,j.to_ip,j.type,j.track,"Token Based Access" as access_base_text, false as terms_popup, "R" as content_download_type, j.uc_id as restricted_user_access_key, "v" as restricted_user_access_type, CASE j.track WHEN 0 THEN false ELSE true END as tracksAccessByVolume,"article" as access_level, j.issue_id, j.article_id from `user_access_article_token` as `j` inner join `user_access_info` as `info` on `j`.`user_access_id` = `info`.`user_access_id` where ((j.from_ip <= 873421719 and j.to_ip >=873421719 )) and ((j.from_date <= 1740474158 and j.to_date >= 1740474158 and j.perpetual= 0) or (j.from_date is null and j.to_date is null and j.perpetual= 1)) and j.article_id = 126152)
      600μsalphaeurekaselec_live_10_06_2022UserAccess.php#1266
      Backtrace
      • 13. app/Models/UserAccess/UserAccess.php:1266
      • 14. app/Http/Controllers/ArticleController.php:2329
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select * from `article` where `first_page` < '993' and is_uploaded = 1 and issue_id = 11935 order by cast(first_page as SIGNED) desc limit 1
      480μsalphaeurekaselec_live_10_06_2022Article.php#1819
      Bindings
      • 0: 993
      Backtrace
      • 14. app/Models/Article.php:1819
      • 15. app/Http/Controllers/ArticleController.php:2470
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 18. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select * from `article` where `last_page` > '1005' and is_uploaded = 1 and issue_id = 11935 order by cast(first_page as SIGNED) asc limit 1
      440μsalphaeurekaselec_live_10_06_2022Article.php#1831
      Bindings
      • 0: 1005
      Backtrace
      • 14. app/Models/Article.php:1831
      • 15. app/Http/Controllers/ArticleController.php:2472
      • 16. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 17. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 18. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select `language_id` from `multilanguage` where `short_code` = 'en'
      160μsalphaeurekaselec_live_10_06_2022ArticleController.php#2497
      Bindings
      • 0: en
      Backtrace
      • 14. app/Http/Controllers/ArticleController.php:2497
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
      • 18. vendor/laravel/framework/src/Illuminate/Routing/Route.php:206
    • select `source_lang_label`, `target_lang_label` from `multilanguage_labels` where `target_lang_id` = 1
      180μsalphaeurekaselec_live_10_06_2022MultiLanguage.php#23
      Bindings
      • 0: 1
      Backtrace
      • 13. app/Models/MultiLanguage.php:23
      • 14. app/Http/Controllers/ArticleController.php:2498
      • 15. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 16. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
    • select `id` from `multilanguage_article` where `article_id` = 126152 limit 1
      2.37msalphaeurekaselec_live_10_06_2022ArticleController.php#2506
      Bindings
      • 0: 126152
      Backtrace
      • 16. app/Http/Controllers/ArticleController.php:2506
      • 17. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
      • 18. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
      • 19. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
      • 20. vendor/laravel/framework/src/Illuminate/Routing/Route.php:206
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` inner join `journal` as `j` on `j`.`journal_id` = `bd`.`content_id` where `b`.`banner_journal` = 1 and `bd`.`content_type` = 'J' and `bd`.`content_id` = 51 and `b`.`banner_from_date` <= 1740474158 and `b`.`banner_to_date` >= 1740474158 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.17msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: J
      • 2: 51
      • 3: 1740474158
      • 4: 1740474158
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select b.banner_link, b.banner_name, b.banner_script, b.banner_remarks, b.banner_file_name, b.banner_placement, bd.content_type, bd.content_id from `tbl_banner` as `b` inner join `tbl_banner_detail` as `bd` on `bd`.`banner_id` = `b`.`banner_id` inner join `users` as `u` on `u`.`id` = `b`.`updated_by` inner join `journal` as `j` on `j`.`journal_id` = `bd`.`content_id` where `b`.`banner_journal` = 1 and `bd`.`content_type` = 'J' and `bd`.`content_id` = 51 and `b`.`banner_from_date` <= 1740474158 and `b`.`banner_to_date` >= 1740474158 and `b`.`banner_status` = 'A' order by `b`.`banner_id` desc
      1.12msalphaeurekaselec_live_10_06_2022Banner.php#95
      Bindings
      • 0: 1
      • 1: J
      • 2: 51
      • 3: 1740474158
      • 4: 1740474158
      • 5: A
      Backtrace
      • 13. app/Models/Banner.php:95
      • 14. app/Http/helpers.php:404
      • 17. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 18. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    • select count(*) as aggregate from `uc_cart_products` where cart_id='d06995145c5cc6586845e355571c5e0c'
      220μsalphaeurekaselec_live_10_06_2022Cart.php#271
      Backtrace
      • 15. app/Models/Cart.php:271
      • 16. view::layouts._header:260
      • 18. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:124
      • 19. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
      • 20. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:73
    App\Models\MultiLanguage
    1MultiLanguage.php#?
        _token
        E9jGSDQjNjdBAOL0qBoKy2n3du8AKUG6jFU5g1vm
        uc_cart_id
        d06995145c5cc6586845e355571c5e0c
        _previous
        array:1 [ "url" => "http://alpha.eurekaselect.com/article/126152" ]
        _flash
        array:2 [ "old" => [] "new" => [] ]
        PHPDEBUGBAR_STACK_DATA
        []
        path_info
        /article/126152
        status_code
        200
        
        status_text
        OK
        format
        html
        content_type
        text/html; charset=UTF-8
        request_query
        []
        
        request_request
        []
        
        request_headers
        0 of 0
        array:9 [ "cookie" => array:1 [ 0 => "wayfindertarget=eyJpdiI6Ikx6bDNXakdyREJvSnhLWm5HYitrQ1E9PSIsInZhbHVlIjoid1FEZ1VSalJSbGk1OHlFQnYwMzRsTnkwYlNHSTdqMzV6eVdlQ2hXK1QxTT0iLCJtYWMiOiIwYmYzZjAzOGQxNjFlMmViMzEwOWU2MTY2ZWVmZGMyZDExZDc5YjRmMzMyNTFkN2FlZTQ5MDY3MDgyMjhhYTBiIiwidGFnIjoiIn0%3Dwayfindertarget=eyJpdiI6Ikx6bDNXakdyREJvSnhLWm5HYitrQ1E9PSIsInZhbHVlIjoid1FEZ1VSalJSbGk1OHlFQnYwMzRsTnkwYlNHSTdqMzV6eVdlQ2hXK1QxTT0iLCJtYWMiOiIwYmYzZjAzOGQxNjFl" ] "accept-encoding" => array:1 [ 0 => "gzip, deflate" ] "accept" => array:1 [ 0 => "text/html,application/xhtml+xml,application/xml;q=0.9,image/avif,image/webp,image/apng,*/*;q=0.8,application/signed-exchange;v=b3;q=0.7" ] "user-agent" => array:1 [ 0 => "Mozilla/5.0 AppleWebKit/537.36 (KHTML, like Gecko; compatible; ClaudeBot/1.0; +claudebot@anthropic.com)" ] "upgrade-insecure-requests" => array:1 [ 0 => "1" ] "cache-control" => array:1 [ 0 => "no-cache" ] "pragma" => array:1 [ 0 => "no-cache" ] "connection" => array:1 [ 0 => "keep-alive" ] "host" => array:1 [ 0 => "alpha.eurekaselect.com" ] ]
        request_cookies
        0 of 0
        array:1 [ "wayfindertarget" => null ]
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