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Current Pharmaceutical Analysis

Editor-in-Chief

ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

Research Article

Evaluation and Application of ZIC-HILIC Columns Selectivity for Four Angiotensin II Receptor Blockers in Pharmaceutical Formulations

Author(s): Ayad K. Fadhil, Ashraf S. Rasheed* and Mohammed Jasim M. Hassan

Volume 18, Issue 9, 2022

Published on: 12 September, 2022

Page: [901 - 908] Pages: 8

DOI: 10.2174/1573412918666220822144157

Price: $65

Abstract

Background: Angiotensin II receptor blockers (ARBs) are helpful medications for treating hypertension and heart failure and have been proposed as a possible alternative to standard angiotensin-converting enzyme inhibitors.

Objective: The present article describes and validates sensitive hydrophilic interaction liquid chromatography methods to simultaneously analyse four angiotensin II receptor blockers: valsartan, telmisartan, losartan, and irbesartan.

Methods: Numerous chromatographic parameters were studied and optimized in detail. As a mobile phase, the best separation was obtained on two handmade columns (ZIC-S1 and ZIC-S4) using acetonitrile/sodium acetate buffer (40 mM, pH 4.75). The detection of target pharmaceuticals was obtained at 220 nm.

Results: The linear ranges of target drugs (valsartan, telmisartan, losartan, and irbesartan) were 0.02-4, 0.06-7, 0.005-6 and 0.08-5 μg/mL on the ZIC-S1 and ZIC-S4 columns, respectively. The suggested methods demonstrated high precision (RSD ≤ 1.15), linearity (r2 ≥ 0.9964), and accuracy (between 98.88 and 101%) with detection and quantitation limits of (0.0021-0.0550 and 0.0063-0.1666 μg/mL, respectively) for all target pharmaceuticals.

Conclusion: The suggested methods have been validated and applied to pharmaceutical formulations, indicating that it is suitable for therapeutic drug monitoring of these medications.

Keywords: Hypertension, angiotensin II, receptor blockers, HILIC, pharmaceutical, formulations.

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