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ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

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Research Article

Evaluating the Cytotoxicity of Monolayered and Multilayered Carbon Nanotubes on Three Different Human Cell Lines

Author(s): Anahita Marhounian Nezhad, Omid Farshad and Mohammad Hossein Morowvat*

Volume 17, Issue 2, 2023

Published on: 13 September, 2022

Page: [186 - 195] Pages: 10

DOI: 10.2174/1872208316666220820121657

Price: $65

Abstract

Background: Nanotechnology and nanobiotechnology have emerged as novel technologies for the production and application of nanoscale materials in different pharmaceutical, medical, and biological fields. Besides, there are a bunch of recently published patents in this field. Although Carbon Nanotubes (CNTs) have various advantages and can be applied for a wide variety of purposes, their toxicity on humans is a matter of concern.

Objective: This study aimed to evaluate six different types of CNTs, including pristine, carboxylated, and hydroxylated single-walled CNTs (SWCNTs) and multi-walled CNTs (MWCNTs) on three human cell lines.

Methods: MTT assay was employed to assess the cytotoxicity of six types of CNTs, including pristine, carboxylated, and hydroxylated forms of SWCNTs and MWCNTs on three different human cell lines.

Results: The findings of the MTT assay showed that the six different types of CNTs (100- 600 μg/mL) exhibited different levels of cytotoxicity on the three human cell lines. The observed trend presented dose-dependent cytotoxicity on the three studied cell lines, including pulmonary, skin, and gastrointestinal cell lines. SWCNT-COOH and MWCNTs accounted for the lowest cell viability in the three human cell lines.

Conclusion: In conclusion, researchers and industrial workers are recommended to be cautious while working with different types of CNT because all their toxicity dimensions have not been determined yet.

Keywords: Carbon nanotube, dose-dependent response, human cell line, toxicity, MTT assay, nanobiotechnology.

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