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Recent Advances in Anti-Infective Drug Discovery

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ISSN (Print): 2772-4344
ISSN (Online): 2772-4352

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Research Article

Physicochemical Properties, Antioxidant and Antimicrobial Activities of Ethiopian Sweet Basil (Ocimum basilicum L.) Leaf and Flower Oil Extracts

Author(s): Getachew Yibeltal, Zekeria Yusuf* and Mulugeta Desta

Volume 17, Issue 2, 2022

Published on: 29 August, 2022

Page: [131 - 138] Pages: 8

DOI: 10.2174/2772434417666220720121051

Price: $65

Abstract

Background: The occurrence of multidrug resistant pathogenic microbes has initiated the development of natural antimicrobial agents from plants. Oils from herbal sources have drawn scientific interest due to their potential source of bioactive compounds.

Objective: This study was aimed to examine the physicochemical and biological activities including antioxidant and antimicrobial potential of the oil extracted from basil leaves and flowers.

Methods: The physicochemical properties of the oil extracts were measured based on oil yield, specific gravity, acid value, free fatty acids and peroxide values whilst the antioxidant activities were assessed by ascorbic acid, DPPH (2, 2- diphenyl-1-picrylhydrazyl), and hydrogen peroxide free radical scavenging activities. The antimicrobial experiment was conducted based on disc diffusion and broth dilution methods.

Results: The result of antioxidant activity of Ocimum basilicum indicated significantly higher DPPH (86.45%) for leaf oil extract. The strongest antibacterial activity with maximum zone of inhibition (15.47 mm), minimum inhibitory concentration MIC (0.09 μg/ml), and corresponding minimum bactericidal concentration MBC (0.19 μg/ml) was exhibited by the flower oil extract against Staphylococcus aureus ATCC-25923. The strongest antifungal activity with maximum zone of inhibition (15.90 mm), MIC (0.125 μg/ml, the least value), and minimum fungicidal concentration MFC (0.09 μg/ml) were recorded for leaf oil against Candida albicans.

Conclusion: It can be concluded from the present study that the sweet basil flower and leaf oil extracts can be potential antioxidant, antibacterial, and antifungal agents.

Keywords: Antibacterial, antifungal, bactericidal concentration, Oil extract, zone of inhibition, Ocimum basilicum L.

« Previous Next »
[1]
Pushpangadan, P.; Bradu, B.L. Advances in horticulture. Medicinal and aromatic plants. New Delhi; Malhotra Publishing House, 1995, 11, pp. 627-657.
[2]
Stanojkovic-Sebic, A.; Dinic, Z. iiicic R, Pivic R, Josic D. Effect of indigenous pseudomonas chlororaphic strains on morphological and main chemical growth parameters of basil (Ocimum basilicum L.). Ratar. Povrt., 2017, 54(2), 42-47.
[http://dx.doi.org/10.5937/ratpov54-12629]
[3]
Opalachenoiva, G.; Obreshkova, D. Comparative studies on the activity of basil an essential oil from Ocimum basilicum L.-against multidrug resistant clinical isolates of the genera Staphylococcus, Enterococcus and Pseudomonas. J. Microbial. Methods, 2003, 54, 105.
[4]
Runyoro, D.; Ngassapa, O.; Vagionas, K.; Aligiannis, N.; Graikou, K.; Chinou, I. Chemical composition and antimicrobial activity essential oils of four Ocimum spp growing in Tanzania. Food Chem., 2010, 119(1), 311-316.
[http://dx.doi.org/10.1016/j.foodchem.2009.06.028]
[5]
Eriotou, E.; Anastasiadou, K.; Nikolopoulos, D.; Koulougliotis, D. Antimicrobial and free radical scavenging activities of basil (Ocimum basilicum) essential oil isolated from five plant varieties growing in Greece. J. Nutr. Food Sci., 2015, 5, 367.
[6]
Labrador, V.; Fernández, F.P.; Pérez Martín, J.M.; Hazen, M.J. Cytotoxicity of butylated hydroxyanisole in Vero cells. Cell Biol. Toxicol., 2007, 23(3), 189-199.
[http://dx.doi.org/10.1007/s10565-006-0153-6] [PMID: 17149658]
[7]
Alemu, A.; Garedew, W.; Gebre, A. Essential oil yield and yield components of basil (Ocimum basilicum L.) as affected by genotype and intrarow spacing at Jimma, SW Ethiopia. Acta Agrobot., 2018, 71(3), 1743.
[http://dx.doi.org/10.5586/aa.1743]
[8]
Yusuf, Z.; Malede, A.; Desta, M.; Idris, M.; Seyida, S. Physicochemical properties and biological activities of mango (Magnifera indica L.) seed kernel and peel oils. Fungal Territory, 2021, 4(4), 1-3.
[9]
Horwitz, W. Official Methods of Analysis. In: 15th ed. Association of Official Chemists’ (AOAC). Washington, DC; , 1990.
[10]
Nielsen, S.S. Introduction to the chemical analysis of foods; Chapman & Hall, 1989, pp. 183-204.
[11]
Loizzo, M.R.; Bonesi, M.; Menichini, F.; Tenuta, M.C.; Leporini, M.; Tundis, R. Antioxidant and carbohydrate-hydrolysing enzymes potential of Sechium edule (Jacq.) Swartz (Cucurbitaceae) peel, leaves and pulp fresh and processed. Plant Foods Hum. Nutr., 2016, 71(4), 381-387.
[http://dx.doi.org/10.1007/s11130-016-0571-4] [PMID: 27474037]
[12]
Bozin, B.; Mimica, D.N.; Samojlik, I.; Goran, A.; Igic, R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem., 2008, 111(4), 925-929.
[http://dx.doi.org/10.1016/j.foodchem.2008.04.071]
[13]
Hsueh, P.R.; Ko, W.C.; Wu, J.J. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial disk susceptibility tests; approved standards – Eleventh Edition. CLSI document M02-A11. In: Clinical and Laboratory Standards Institute. Wayne, PA, 19087 USA , 2012.
[14]
Hudzicki, J. Kirby-bauer disk diffusion susceptibility test protocol. Am Soc Microbiol, 2009, 15, 55-56.
[15]
Andrews, J.M. Determination of minimum inhibitory concentrations. J. Antimicrob. Chemother., 2001, 48(Suppl. 1), 5-16.
[http://dx.doi.org/10.1093/jac/48.suppl_1.5] [PMID: 11420333]
[16]
SAS Institute. SAS enterprise guide, Version 92; SAS Inst.: Cary, NC, USA, 2011.
[17]
Alfawaz, M.A. Chemical composition and oil characteristics of pumpkin (Cucurbita maxima) seed kernels., Food Sci Agric Res Center. King Saud Univ, 2004, 2(1), 5-18.
[18]
Ajayi, I.A.; Oderinde, R.A.; Kajogbola, D.O.; Uponi, J.I. Oil content and fatty acid composition of some underutilized legumes from Nigeria. Food Chem., 2006, 99(1), 115-120.
[http://dx.doi.org/10.1016/j.foodchem.2005.06.045]
[19]
Markovic, V.V.; Bastic, L.V. Characteristics of pumpkin seed oil. J. Am. Oil Chem. Soc., 1975, 53(1), 42-44.
[http://dx.doi.org/10.1007/BF02632524]
[20]
Olayemi, R.F.; Jawonisi, I.O.; Samuel, J.A. Characterization and physicochemical analysis of essential oil of Cymbopogon citrates leaves. Bayero J. Pure Appl. Sci., 2018, 11(1), 74-81.
[http://dx.doi.org/10.4314/bajopas.v11i1.14]
[21]
Stanojevic, L.P.; Marjanovic-Balaban, Z.R.; Kalaba, V.D.; Stanojevic, J.S.; Cvetkovic, D.J.; Cakic, M.D. Chemical composition, antioxidant and antimicrobial activity of basil (Ocimum basilicum L.) essential oil. J. Essent. Oil-Bear. Plants, 2017, 20(6), 1557-1569.
[http://dx.doi.org/10.1080/0972060X.2017.1401963]
[22]
Sanchez-Moreno, C. Review: Methods used to evaluate the free radical scavenging activity in foods and biological systems. Food Sci. Technol. Int., 2002, 8(3), 121-137.
[http://dx.doi.org/10.1177/1082013202008003770]
[23]
Molyneux, P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol., 2004, 26(2), 211-219.
[24]
Hanif, M.A.; Al-Maskari, M.Y.; Al-Maskari, A.; Al-Shukaili, A.; Al-Maskari, A.Y. Essential oil composition, antimicrobial and antioxidant activities of unexplored Omani basil. J. Med. Plants Res., 2011, 5(5), 751-757.
[25]
Politeo, O.; Jukic, M.; Milos, M. Chemical composition and antioxidant capacity of free volatile aglycones from basil (Ocimum basilicum L.) compared with its essential oil. Food Chem., 2007, 101(1), 379-385.
[http://dx.doi.org/10.1016/j.foodchem.2006.01.045]
[26]
Sakkas, H.; Papadopoulou, C. Antimicrobial activity of basil, oregano, and thyme essential oils. J. Microbiol. Biotechnol., 2017, 27(3), 429-438.
[http://dx.doi.org/10.4014/jmb.1608.08024] [PMID: 27994215]

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