Abstract
Objectives: Glutamatergic dysfunction has been implicated in the pathogenesis of numerous central nervous system disorders. Artemisia absinthium (A. absinthium), has been reported to have neuroprotective and antioxidant activities. The current study examined the effect of A. absinthium extract on glutamate-induced cytotoxicity using PC12 cells.
Methods: PC12 cells were pretreated with A. absinthium extract (70% ethanol) at concentration ranges of 6.25-25 μg/ml for 2 h, followed by exposure to glutamate (8 mM) for 24 hr. The cells viability, reactive oxygen species (ROS) accumulation, and lipid peroxidation were measured. Moreover, a propidium iodide flow cytometry assay was performed for the assessment of apoptosis.
Results: Pretreatment with A. absinthium extract markedly attenuated the loss of cell viability, ROS generation, lipid peroxidation, and cell apoptosis in glutamate-injured PC-12 cells.
Conclusion: Our results indicate that A. absinthium extract protects PC12 cells against glutamate-induced injury, possibly by reducing oxidative stress and apoptosis.
Keywords: Artemisia absinthium, PC12 cells, glutamate, antioxidant, apoptosis, central nervous system disorders.
Graphical Abstract
[1]
Chang, C-H.; Chen, H-X.; Yü, G.; Peng, C-C.; Peng, R.Y. Curcumin-protected PC12 cells against glutamate-induced oxidative toxicity. Food Technol. Biotechnol., 2014, 52(4), 468-478.
[http://dx.doi.org/10.17113/ftb.52.04.14.3622] [PMID: 27904320]
[http://dx.doi.org/10.17113/ftb.52.04.14.3622] [PMID: 27904320]
[2]
Ma, S.; Liu, H.; Jiao, H.; Wang, L.; Chen, L.; Liang, J.; Zhao, M.; Zhang, X. Neuroprotective effect of ginkgolide K on glutamate-induced cytotoxicity in PC 12 cells via inhibition of ROS generation and Ca(2+) influx. Neurotoxicology, 2012, 33(1), 59-69.
[http://dx.doi.org/10.1016/j.neuro.2011.11.003] [PMID: 22120026]
[http://dx.doi.org/10.1016/j.neuro.2011.11.003] [PMID: 22120026]
[3]
Jiang, G.; Wu, H.; Hu, Y.; Li, J.; Li, Q. Gastrodin inhibits glutamate-induced apoptosis of PC12 cells via inhibition of CaMKII/ASK-1/p38 MAPK/p53 signaling cascade. Cell. Mol. Neurobiol., 2014, 34(4), 591-602.
[http://dx.doi.org/10.1007/s10571-014-0043-z] [PMID: 24619207]
[http://dx.doi.org/10.1007/s10571-014-0043-z] [PMID: 24619207]
[4]
Batiha, G.E-S.; Olatunde, A.; El-Mleeh, A.; Hetta, H.F.; Al-Rejaie, S.; Alghamdi, S.; Zahoor, M.; Magdy Beshbishy, A.; Murata, T.; Zaragoza-Bastida, A.; Rivero-Perez, N. Bioactive compounds, pharmacological actions, and pharmacokinetics of wormwood (Artemisia absinthium). Antibiotics (Basel), 2020, 9(6), 353.
[http://dx.doi.org/10.3390/antibiotics9060353] [PMID: 32585887]
[http://dx.doi.org/10.3390/antibiotics9060353] [PMID: 32585887]
[5]
Canadanovicq Brunet, J.M.; Djilas, S.M.; Cetkovic, G.S.; Tumbas, V.T. Freeq radical scavenging activity of wormwood (Artemisia absinthium L) extracts. J. Sci. Food Agric., 2005, 85(2), 265-272.
[http://dx.doi.org/10.1002/jsfa.1950]
[http://dx.doi.org/10.1002/jsfa.1950]
[6]
Hadi, A.; Hossein, N.; Shirin, P.; Najmeh, N.; Abolfazl, M. Anti-inflammatory and analgesic activities of Artemisia absinthium and chemical composition of its essential oil. Int. J. Pharm. Sci. Rev. Res., 2014, 38, 237-244.
[7]
Bora, K.S.; Sharma, A. Neuroprotective effect of Artemisia absinthium L. on focal ischemia and reperfusion-induced cerebral injury. J. Ethnopharmacol., 2010, 129(3), 403-409.
[http://dx.doi.org/10.1016/j.jep.2010.04.030] [PMID: 20435123]
[http://dx.doi.org/10.1016/j.jep.2010.04.030] [PMID: 20435123]
[8]
Zeraati, F.; Esna-Ashari, F.; Araghchian, M.; Emam, A.H.; Rad, M.V.; Seif, S. Evaluation of topical antinociceptive effect of Artemisia absinthium extract in mice and possible mechanisms. Afr. J. Pharm. Pharmacol., 2014, 8(19), 492-496.
[9]
Rakhshandeh, H.; Heidari, A.; Pourbagher-Shahri, A.M.; Rashidi, R.; Forouzanfar, F. Hypnotic Effect of A. absinthium hydroalcoholic extract in pentobarbital-treated mice. Neurol. Res. Int., 2021, 2021, 5521019.
[10]
Aberham, A.; Cicek, S.S.; Schneider, P.; Stuppner, H. Analysis of sesquiterpene lactones, lignans, and flavonoids in wormwood (Artemisia absinthium L.) using high-performance liquid chromatography (HPLC)-mass spectrometry, reversed phase HPLC, and HPLC-solid phase extraction-nuclear magnetic resonance. J. Agric. Food Chem., 2010, 58(20), 10817-10823.
[http://dx.doi.org/10.1021/jf1022059] [PMID: 20886883]
[http://dx.doi.org/10.1021/jf1022059] [PMID: 20886883]
[11]
Sadeghnia, H.R.; Kolangikhah, M.; Asadpour, E.; Forouzanfar, F.; Hosseinzadeh, H. Berberine protects against glutamate-induced oxidative stress and apoptosis in PC12 and N2a cells. Iran. J. Basic Med. Sci., 2017, 20(5), 594-603.
[PMID: 28656094]
[PMID: 28656094]
[12]
Forouzanfar, F.; Asadpour, E.; Hosseinzadeh, H.; Boroushaki, M.T.; Adab, A.; Dastpeiman, S.H.; Sadeghnia, H.R. Safranal protects against ischemia-induced PC12 cell injury through inhibiting oxidative stress and apoptosis. Naunyn Schmiedebergs Arch. Pharmacol., 2021, 394(4), 707-716.
[http://dx.doi.org/10.1007/s00210-020-01999-8] [PMID: 33128592]
[http://dx.doi.org/10.1007/s00210-020-01999-8] [PMID: 33128592]
[13]
Rashidi, R.; Ghorbani, A.; Rakhshandeh, H.; Mousavi, S.H. Protective effect of Artemisia absinthium on 6-hydroxydopamine-induced toxicity in SH-SY5Y cell line. Avicenna J. Phytomed., 2021, 11(3), 238-246.
[PMID: 34046320]
[PMID: 34046320]
[14]
Ranjbar, M.; Naghavi, M.R.; Alizadeh, H. Chemical composition of the essential oils of Artemisia species from Iran: A comparative study using multivariate statistical analysis. J. Essent. Oil Res., 2020, 32(4), 361-371.
[http://dx.doi.org/10.1080/10412905.2020.1750495]
[http://dx.doi.org/10.1080/10412905.2020.1750495]
[15]
Mathlouthi, A.; Belkessam, M.; Sdiri, M.; Diouani, M.F.; Souli, A.; El-Bok, S.; Ben-Attia, M. Chemical composition and anti-leishmania major activity of essential oils from artemesia spp. grown in central tunisia. J. Essent. Oil-Bear. Plants, 2018, 21(5), 1186-1198.
[http://dx.doi.org/10.1080/0972060X.2018.1526128]
[http://dx.doi.org/10.1080/0972060X.2018.1526128]
[16]
Arai, T.; Toda, Y.; Kato, K.; Miyamoto, K.; Hasegawa, T.; Yamada, K.; Ueda, J.; Hasegawa, K.; Inoue, T.; Shigemori, H. Artabolide, a novel polar auxin transport inhibitor isolated from Artemisia absinthium. Tetrahedron, 2013, 69(34), 7001-7005.
[http://dx.doi.org/10.1016/j.tet.2013.06.052]
[http://dx.doi.org/10.1016/j.tet.2013.06.052]
[17]
Beauhaire, J.; Fourrey, J.; Vuilhorgne, M.; Lallemand, J. Dimeric sesquiterpene lactones: Structure of absinthin. Tetrahedron Lett., 1980, 21(33), 3191-3194.
[http://dx.doi.org/10.1016/S0040-4039(00)77442-9]
[http://dx.doi.org/10.1016/S0040-4039(00)77442-9]
[18]
Marmiroli, P.; Cavaletti, G. The glutamatergic neurotransmission in the central nervous system. Curr. Med. Chem., 2012, 19(9), 1269-1276.
[http://dx.doi.org/10.2174/092986712799462711] [PMID: 22338563]
[http://dx.doi.org/10.2174/092986712799462711] [PMID: 22338563]
[19]
Dong, X.X.; Wang, Y.; Qin, Z.H. Molecular mechanisms of excitotoxicity and their relevance to pathogenesis of neurodegenerative diseases. Acta Pharmacol. Sin., 2009, 30(4), 379-387.
[http://dx.doi.org/10.1038/aps.2009.24] [PMID: 19343058]
[http://dx.doi.org/10.1038/aps.2009.24] [PMID: 19343058]
[20]
Riccardi, C.; Nicoletti, I. Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat. Protoc., 2006, 1(3), 1458-1461.
[http://dx.doi.org/10.1038/nprot.2006.238] [PMID: 17406435]
[http://dx.doi.org/10.1038/nprot.2006.238] [PMID: 17406435]
[21]
Hussain, M.; Raja, N.I.; Akram, A.; Iftikhar, A.; Ashfaq, D.; Yasmeen, F.; Mazhar, R.; Imran, M.; Iqbal, M. A status review on the pharmacological implications of Artemisia absinthium: A critically endangered plant. Asian Pac. J. Trop. Dis., 2017, 7(3), 185-192.
[http://dx.doi.org/10.12980/apjtd.7.2017D6-385]
[http://dx.doi.org/10.12980/apjtd.7.2017D6-385]
[22]
Craciunescu, O.; Constantin, D.; Gaspar, A.; Toma, L.; Utoiu, E.; Moldovan, L. Evaluation of antioxidant and cytoprotective activities of Arnica montana L. and Artemisia absinthium L. ethanolic extracts. Chem. Cent. J., 2012, 6(1), 97.
[http://dx.doi.org/10.1186/1752-153X-6-97] [PMID: 22958433]
[http://dx.doi.org/10.1186/1752-153X-6-97] [PMID: 22958433]
[23]
Hallal, N.; Kharoubi, O.; Benyettou, I.; Tair, K.; Ozaslan, M.; Aoues, A. In vivo amelioration of oxidative stress by Artemisia absinthium L. administration on mercuric chloride toxicity in brain regions. J. Biol. Sci. (Faisalabad, Pak.), 2016, 16(5), 167-177.
[http://dx.doi.org/10.3923/jbs.2016.167.177]
[http://dx.doi.org/10.3923/jbs.2016.167.177]
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