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ISSN (Print): 1872-2083
ISSN (Online): 2212-4012

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Research Article

Physicochemical Properties and Biological Activities of Koseret (Lippia adoensis Hochst. Var. Koseret) Seed and Leaf Oil Extracts

Author(s): Mekdes Shiferaw, Zekeria Yusuf* and Mulugeta Desta

Volume 17, Issue 2, 2023

Published on: 03 September, 2022

Page: [142 - 150] Pages: 9

DOI: 10.2174/1872208316666220617104318

Price: $65

Abstract

Background: Lippia adoensis Hoechst var. adoensis (wild variety) and variety koseret (cultivated variety) have been used as traditional medicine, condiments, and endemic to Ethiopia.

Objective: This study aimed to assess the physicochemical properties and biological activities of oil extracts from seed and leaves of koseret (L. adoensis var. koseret).

Methodology: Soxhlet apparatus was used for oil extraction using hexane as a solvent. The oil quality assessment was based on oil yield, acid value, percent free fatty acid, and peroxide value, while the biological activities were investigated based on antioxidant and antimicrobial activities. The antimicrobial experiment was arranged as 2 x1x4 in a completely randomized factorial design with three replications.

Results: The result indicated that significantly higher oil yield (2.25%), acid value (2.66%) and free fatty acid (1.34%) were recorded for seed oil using the solvent extraction method. Leaf oil was recorded to have significantly higher values of DPPH (2,2- diphenyl-1- picrylhydrazyl), ascorbic acid and total carotenoid contents, but a lower value of hydrogen peroxide scavenging activity indicated that leaf oil presented higher antioxidant activity than seed oil in koseret. The koseret leaf oil demonstrated stronger antibacterial activity with a maximum zone of inhibition (14.50±0.21 mm), minimum inhibitory concentration (MIC, 0.25 μg/ml) and corresponding minimum bactericidal concentration (MBC, 0.25 μg/ml) against S. aureus. Furthermore, leaf oil has also presented stronger antifungal activity with a maximum zone of inhibition (14.83 mm), MIC (0.25 μg/ml), and minimum fungicidal concentration (MFC, 0.50 μg/ml) against Aspergillus versicolor.

Conclusion: It can be concluded from the results of this study that leaf oil extract has demonstrated better biological activities, including both antioxidant and antimicrobial potentials, than seed oil in koseret.

Keywords: Antimicrobial, antioxidant, bactericidal, fungicidal, total carotenoids, ethiopian.

« Previous Next »
[1]
Yogayata SP, Vijay DW. Herbal medicines and nutritional supplements used in the treatment of glaucoma. Res J Pharm Biol Chem Sci 2012; 3(1): 331.
[2]
Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001; 109 (Suppl. 1): 69-75.
[PMID: 11250806]
[3]
Tapsell LC, Hemphill I, Cobiac L, et al. Health benefits of herbs and spices: The past, the present, the future. Med J Aust 2006; 185(S4): S1-S24.
[http://dx.doi.org/10.5694/j.1326-5377.2006.tb00548.x] [PMID: 17022438]
[4]
Verdcourt B. Verbenaceae Flora of East Africa. Rotterdam, Brookfield: Balkema A. A. 1992; p. 27.
[5]
Demissew S. A confusion in Lippia (Verbenaceae) in Tropical Africa. Kew Bull 1993; 48(2): 375-9.
[http://dx.doi.org/10.2307/4117945]
[6]
Tadeg H, Mohammed E, Asres K, Gebre-Mariam T. Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. J Ethnopharmacol 2005; 100(1-2): 168-75.
[http://dx.doi.org/10.1016/j.jep.2005.02.031] [PMID: 16054532]
[7]
Megersa M, Asfaw Z, Kelbessa E, Beyene A, Woldeab B. An ethnobotanical study of medicinal plants in Wayu Tuka District, East Welega Zone of Oromia Regional State, West Ethiopia. J Ethnobiol Ethnomed 2013; 9(1): 68.
[http://dx.doi.org/10.1186/1746-4269-9-68] [PMID: 24295044]
[8]
Riot S, Mariamawit Y, Ameha S, Kaleab A. Radical scavenging activity of volatile oils of herbs traditionally used to spice cooking butter in Ethiopia. Ethiopian Pharmaceutical Journal 2005; 23: 7-14.
[9]
Nigist A, Sebsebe D. Aromatic Plants of Ethiopia. Addis Ababa, Ethiopia: Shama Books 2009; pp. 137-8.
[10]
van Wyk and Ben-Erik. Culinary Herbs and Spices of the World. Chicago, IL: University of Chicago Press 2014; 9.
[11]
Buli GA, Duga AG, Dessalegn E. Antimicrobial activity of Lippia adoensis var. koseret against human pathogenic bacteria and fungi. Am J of Clin Exp Med 2015; 3(3): 118-23.
[http://dx.doi.org/10.11648/j.ajcem.20150303.18]
[12]
Gemeda N, Woldeamanuel Y, Asrat D, Debella A, Belete Y. Assessment of Lippia adoensis Hochst. Var. Koseret, Rosmarinus officinalis and Ruta chalepensis. Essential oils as a potential source of fungitoxic and mycosporicidal activity against toxigenic Aspergillus spp. Archives 2015; 2: 85-94.
[13]
Germame Y, Mekuria T. Identification of bioactive compound, phytochemical analysis and antimicrobial activity of Lippia adoensis var. koseret from Ethiopia. Sch Int J Tradit Complement Med 2021; 4(8): 139-53.
[14]
Fikadu Y. Investigation of chemical constituents and antioxidant activities of the essential oils of Lippia adoensis and Ocimum sanctum L. M.Sc Thesis, Addis Ababa University 2020.
[15]
Official Methods of Analysis of the AOAC International. (7th ed.). Gaithersburg, Maryland, USA 2000; II.
[16]
Nielsen SS. Introduction to the Chemical Analysis of Foods 2002; 183-204.
[17]
Loizzo MR, Bonesi M, Menichini F, Tenuta MC, Leporini M, Tundis R. Antioxidant and carbohydrate-hydrolysing enzymes potential of Sechium edule (Jacq.) Swartz (Cucurbitaceae) peel, leaves and pulp fresh and processed. Plant Foods Hum Nutr 2016; 71(4): 381-7.
[http://dx.doi.org/10.1007/s11130-016-0571-4] [PMID: 27474037]
[18]
Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem 2008; 111(4): 925-9.
[http://dx.doi.org/10.1016/j.foodchem.2008.04.071]
[19]
Official Methods of Analysis. Association of Official Chemists. 15th ed. Washington, DC, USA: AOAC 1990.
[20]
Nagata M, Yamashita I. Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit. Japan J Food Eng 1992; 39(10): 925-8.
[21]
Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001; 48 (Suppl. 1): 5-16.
[http://dx.doi.org/10.1093/jac/48.suppl_1.5] [PMID: 11420333]
[22]
Morshed H, Islam MS, Parvin S, et al. Antimicrobial and cytotoxic activity of the methanol extract of Paederia foetida Linn. (Rubiaceae). J Appl Pharm Sci 2012; 02(01): 77-80.
[23]
SAS Institute. SAS enterprise guide, Version 92. Cary, NC, USA: SAS Inst. 2011.
[24]
Vlietinck AJ, Van Hoof L, Totté J, et al. Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. J Ethnopharmacol 1995; 46(1): 31-47.
[http://dx.doi.org/10.1016/0378-8741(95)01226-4] [PMID: 7475121]
[25]
Hsieh PC, Siegel SA, Rogers B, Davis D, Lewis K. Bacteria lacking a multidrug pump: A sensitive tool for drug discovery. Proc Natl Acad Sci 1998; 95(12): 6602-6.
[http://dx.doi.org/10.1073/pnas.95.12.6602] [PMID: 9618458]
[26]
Chérigo L, Pereda-Miranda R, Fragoso-Serrano M, Jacobo-Herrera N, Kaatz GW, Gibbons S. Inhibitors of bacterial multidrug efflux pumps from the resin glycosides of Ipomoea murucoides. J Nat Prod 2008; 71(6): 1037-45.
[http://dx.doi.org/10.1021/np800148w] [PMID: 18500841]
[27]
Kumar A, Khan IA, Koul S, et al. Novel structural analogues of piperine as inhibitors of the NorA efflux pump of Staphylococcus aureus. J Antimicrob Chemother 2008; 61(6): 1270-6.
[http://dx.doi.org/10.1093/jac/dkn088] [PMID: 18334493]

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