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Current Pharmaceutical Design

Editor-in-Chief

ISSN (Print): 1381-6128
ISSN (Online): 1873-4286

Research Article

Effects of Musk Volatile Compounds on Attenuated Nerve Injury and Improving Post-cerebral Ischemic Exercise Functions

Author(s): Dan Wang, Meng-Meng Zhang, Chun-Jie Wu*, Qi Liang, Da-Neng Wei, Lin He and Xun Ye

Volume 28, Issue 23, 2022

Published on: 15 July, 2022

Page: [1932 - 1948] Pages: 17

DOI: 10.2174/1381612828666220526154014

Price: $65

Abstract

Background: Reperfusion Injury Acute ischemic stroke is increasing in people recently and Musk, as a commonly used Traditional Chinese Medicine (TCM), has been suggested as a potential agent against acute ischemic stroke, but the efficacies and underlying mechanisms of it remain unknown.

Objective: This study was aimed to test the hypotheses that volatile compounds of musk could attenuate nerve injury and identify the bioactive compounds and potential mechanisms of Musk.

Methods: Transient middle cerebral artery occlusion (MCAO) model in vivo in Sprague-Dawley rats (SD rats) was used to test this hypothesis. Collecting ingredients of Musk and their related targets were discerned from the Gas chromatography-olfactory mass spectrometry (GC-O-MS) experiment. Then the potential mechanisms and targets of the compounds were searched by network pharmacology techniques. Finally, the pathway was verified by Western Bolt (WB).

Results: First, Musk treatment significantly up-regulated the relative levels of AKT1, PI3KA, and VEGFA in the hippocampus, and improved the sport functions in the post-MCAO ischemic rats in vivo. Next, twenty potential flavor active compounds were recognized by GC-O-MS. A total of 89 key targets including HIF-1, PIK3CA, TNF signaling pathway, and VEGF were identified. AKT1, HIF1A, PIK3CA, and VEGFA were viewed as the most important genes, which were validated by molecular docking simulation.

Conclusion: The Volatile compounds of musk can attenuate nerve injury and improving post-cerebral ischemic exercise functions by HIF1A pathways, and the combined data provide novel insight for Musk volatile compounds developed as new drug for improving reperfusion injury in acute ischemic stroke.

Keywords: Musk, reperfusion injury, GC-O-MS, GO enrichment, KEGG enrichment, molecular docking.

« Previous
[1]
Winstein CJ, Stein J, Arena R, et al. Amer heart assoc stroke C, council cardiovasc stroke N, council clin C, and council quality care outcomes R. Guidelines for adult stroke rehabilitation and recovery a guideline for healthcare professionals from the american heart association/american stroke association. Stroke 2016; 47(6): E98-E169.
[http://dx.doi.org/10.1161/STR.0000000000000098] [PMID: 27145936]
[2]
Katan M, Luft A. Global burden of stroke. Semin Neurol 2018; 38(2): 208-11.
[http://dx.doi.org/10.1055/s-0038-1649503] [PMID: 29791947]
[3]
Powers WJ, Derdeyn CP, Biller J, et al. 2015 american heart association/american stroke association focused update of the 2013 guidelines for the early management of patients with acute ischemic stroke regarding endovascular treatment: A guideline for healthcare professionals from the american heart association/american stroke association. Stroke 2015; 46(10): 3020-35.
[http://dx.doi.org/10.1161/STR.0000000000000074] [PMID: 26123479]
[4]
Liu Z, Chopp M. Astrocytes, therapeutic targets for neuroprotection and neurorestoration in ischemic stroke. Prog Neurobiol 2016; 144: 103-20.
[http://dx.doi.org/10.1016/j.pneurobio.2015.09.008] [PMID: 26455456]
[5]
Wardlaw JM, Murray V, Berge E, del Zoppo GJ. Thrombolysis for acute ischaemic stroke. Cochrane Db Syst Rev 2014; p. 7.
[6]
Prabhakaran S, Ruff I, Bernstein RA. Acute stroke intervention: A systematic review. JAMA 2015; 313(14): 1451-62.
[http://dx.doi.org/10.1001/jama.2015.3058] [PMID: 25871671]
[7]
Commission CP. Pharmacopoeia of the People’s Republic of China Part 1. Beijing: People's Medical Publishing House 2020.
[8]
Liu K, Xie L, Deng M, Zhang X, Luo J, Li X. Zoology, chemical composition, pharmacology, quality control and future perspective of Musk (Moschus): A review. Chin Med 2021; 16(1): 46-6.
[http://dx.doi.org/10.1186/s13020-021-00457-8] [PMID: 34147113]
[9]
Zhang C, Liao Y, Liu L, et al. A network pharmacology approach to investigate the active compounds and mechanisms of musk for ischemic stroke. Evid Based Complement Alternat Med 2020; 2020: 4063180-0.
[http://dx.doi.org/10.1155/2020/4063180] [PMID: 32714405]
[10]
Xu L, Cao Y. Native musk and synthetic musk ketone strongly induced the growth repression and the apoptosis of cancer cells. BMC Complement Altern Med 2016; 16(1): 511-1.
[http://dx.doi.org/10.1186/s12906-016-1493-2] [PMID: 27931220]
[11]
Gane S, Georganakis D, Maniati K, et al. Molecular vibration-sensing component in human olfaction. PLoS One 2013; 8(1): e55780-0.
[http://dx.doi.org/10.1371/journal.pone.0055780] [PMID: 23372854]
[12]
Song H, Liu J. GC-O-MS technique and its applications in food flavor analysis. Food Res Int 2018; 114: 187-98.
[http://dx.doi.org/10.1016/j.foodres.2018.07.037] [PMID: 30361015]
[13]
Liang J, Wang Q, Li JQ, et al. Long non-coding RNA MEG3 promotes cerebral ischemia-reperfusion injury through increasing pyroptosis by targeting miR-485/AIM2 axis. Exp Neurol 2020; 325: 113139.
[http://dx.doi.org/10.1016/j.expneurol.2019.113139] [PMID: 31794744]
[14]
Zhang X, Yuan M, Yang S, et al. Enriched environment improves post-stroke cognitive impairment and inhibits neuroinflammation and oxidative stress by activating Nrf2-ARE pathway. Int J Neurosci 2021; 131(7): 641-9.
[http://dx.doi.org/10.1080/00207454.2020.1797722] [PMID: 32677581]
[15]
Wang WW, Xie CL, Lu L, et al. A systematic review and meta-analysis of Baihui (GV20)-based scalp acupuncture in experimental ischemic stroke. Sci Rep 2014; 4(1): 3981-1.
[http://dx.doi.org/10.1038/srep03981] [PMID: 24496233]
[16]
Ai Q, Chen C, Chu S, et al. IMM-H004 protects against cerebral ischemia injury and cardiopulmonary complications via CKLF1 mediated inflammation pathway in adult and aged rats. Int J Mol Sci 2019; 20(7): 1661.
[http://dx.doi.org/10.3390/ijms20071661] [PMID: 30987181]
[17]
Majithia D, Metrani R, Dhowlaghar N, et al. Assessment and classification of volatile profiles in melon breeding lines using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry. Plants 2021; 10(10): 2166.
[http://dx.doi.org/10.3390/plants10102166] [PMID: 34685975]
[18]
Kesen S, Amanpour A, Tsouli Sarhir S, et al. Characterization of aroma-active compounds in seed extract of black cumin (Nigella sativa L.) by aroma extract dilution analysis. Foods 2018; 7(7): 98.
[http://dx.doi.org/10.3390/foods7070098] [PMID: 29954052]
[19]
Lasekan O, Teoh LS. Contribution of aroma compounds to the antioxidant properties of roasted white yam (Dioscorea rotundata). BMC Chem 2019; 13(1): 133-3.
[http://dx.doi.org/10.1186/s13065-019-0650-3] [PMID: 31891159]
[20]
Daina A, Michielin O, Zoete V. SwissTargetPrediction: Updated data and new features for efficient prediction of protein targets of small molecules. Nucleic Acids Res 2019; 47(W1): W357-64.
[http://dx.doi.org/10.1093/nar/gkz382] [PMID: 31106366]
[21]
Tao W, Xu X, Wang X, et al. Network pharmacology-based prediction of the active ingredients and potential targets of Chinese herbal Radix Curcumae formula for application to cardiovascular disease. J Ethnopharmacol 2013; 145(1): 1-10.
[http://dx.doi.org/10.1016/j.jep.2012.09.051] [PMID: 23142198]
[22]
Casas AI, Hassan AA, Larsen SJ, et al. From single drug targets to synergistic network pharmacology in ischemic stroke. Proc Natl Acad Sci USA 2019; 116(14): 7129-36.
[http://dx.doi.org/10.1073/pnas.1820799116] [PMID: 30894481]
[23]
Rappaport N, Fishilevich S, Nudel R, et al. Rational confederation of genes and diseases: NGS interpretation via GeneCards, MalaCards and VarElect. Biomed Eng Online 2017; 16(S1)(Suppl. 1): 72-2.
[http://dx.doi.org/10.1186/s12938-017-0359-2] [PMID: 28830434]
[24]
Wishart DS, Feunang YD, Guo AC, et al. DrugBank 5.0: A major update to the DrugBank database for 2018. Nucleic Acids Res 2018; 46(D1): D1074-82.
[http://dx.doi.org/10.1093/nar/gkx1037] [PMID: 29126136]
[25]
Amberger JS, Bocchini CA, Schiettecatte F, Scott AF, Hamosh A. OMIM.org: Online mendelian inheritance in man (OMIM®), an online catalog of human genes and genetic disorders. Nucleic Acids Res 2015; 43: D789-98.
[http://dx.doi.org/10.1093/nar/gku1205] [PMID: 25428349]
[26]
Li S, Zhang B. Traditional chinese medicine network pharmacology: Theory, methodology and application. Chin J Nat Med 2013; 11(2): 110-20.
[http://dx.doi.org/10.1016/S1875-5364(13)60037-0] [PMID: 23787177]
[27]
Niu WH, Wu F, Cao WY, et al. Network pharmacology for the identification of phytochemicals in traditional Chinese medicine for COVID-19 that may regulate interleukin-6. Biosci Rep 2021; 41(1): BSR20202583.
[http://dx.doi.org/10.1042/BSR20202583] [PMID: 33146673]
[28]
Kanehisa M, Furumichi M, Sato Y, Ishiguro-Watanabe M, Tanabe M. KEGG: integrating viruses and cellular organisms. Nucleic Acids Res 2021; 49(D1): D545-51.https://pubmed.ncbi.nlm.nih.gov/33125081/
[PMID: 33125081]
[29]
Liu ZY, Guo FF, Wang Y, et al. BATMAN-TCM: a Bioinformatics Analysis Tool for Molecular mechANism of Traditional Chinese Medicine. Sci Rep-Uk 2016; p. 6.
[30]
Saikia S, Bordoloi M. Molecular docking: Challenges, advances and its use in drug discovery perspective. Curr Drug Targets 2019; 20(5): 501-21.
[http://dx.doi.org/10.2174/1389450119666181022153016] [PMID: 30360733]
[31]
Meng XY, Zhang HX, Mezei M, et al. Molecular docking: A powerful approach for structure-based drug discovery. Curr Computeraided Drug Des 2011; 7(2): 146-57.
[http://dx.doi.org/10.2174/157340911795677602] [PMID: 21534921]
[32]
Liu Z, Li H, Hong C, et al. ALS-Associated E478G mutation in human OPTN (Optineurin) promotes inflammation and induces neuronal cell death. Front Immunol 2018; 9: 2647.
[http://dx.doi.org/10.3389/fimmu.2018.02647] [PMID: 30519240]
[33]
Hamm RJ, White-Gbadebo DM, Lyeth BG, et al. The effect of age on motor and cognitive deficits after traumatic brain injury in rats. Neurosurgery 1992; 31(6): 1072-7.
[PMID: 1335138]
[34]
Dash PK, Orsi SA, Zhang M, et al. Valproate administered after traumatic brain injury provides neuroprotection and improves cognitive function in rats. PLoS One 2010; 5(6): e11383.
[http://dx.doi.org/10.1371/journal.pone.0011383] [PMID: 20614021]
[35]
Löwhagen Hendén P, Rentzos A, Karlsson JE, et al. General anesthesia versus conscious sedation for endovascular treatment of acute ischemic stroke: The anstroke trial (Anesthesia During Stroke). Stroke 2017; 48(6): 1601-7.
[http://dx.doi.org/10.1161/STROKEAHA.117.016554] [PMID: 28522637]
[36]
Sun R, Zhang Z, Huang W, et al. Protective effects and machanism of muskone on pheochromocytoma cell injure induced by glutamate. Zhongguo Zhongyao Zazhi 2009; 34(13): 1701-4.
[PMID: 19873786]
[37]
Petrovic-Djergovic D, Goonewardena SN, Pinsky DJ. Inflammatory disequilibrium in stroke. Circ Res 2016; 119(1): 142-58.
[http://dx.doi.org/10.1161/CIRCRESAHA.116.308022] [PMID: 27340273]
[38]
Sheldon RA, Osredkar D, Lee CL, et al. HIF-1 alpha-deficient mice have increased brain injury after neonatal hypoxia-ischemia. Dev Neurosci 2009; 31(5): 452-8.
[http://dx.doi.org/10.1159/000232563] [PMID: 19672073]
[39]
Liang X, Liu X, Lu F, et al. HIF1α signaling in the endogenous protective responses after neonatal brain hypoxia-ischemia. Dev Neurosci 2019; 1-10.
[PMID: 30836371]

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