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Current Pharmaceutical Biotechnology

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ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

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Research Article

Syringic Acid Suppressed Proliferation, Invasion, and Migration via Inhibition of Matrix Metalloproteinases Expression on Glioblastoma Cells by Promoting Apoptosis

Author(s): Dan Li, Dongdong Luo, Su Hu, Hailin Zhao and Biao Peng*

Volume 24, Issue 2, 2023

Published on: 02 August, 2022

Page: [310 - 316] Pages: 7

DOI: 10.2174/1389201023666220513100129

Price: $65

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Abstract

Background: Human brain tumor glioblastoma (GBM) is the most hostile malignancy, currently lacking a successful cure and good prognosis.

Objective: To examine the anticancer effects of syringic acid (SA) on human cancer GBM cells.

Methodology: The different doses of SA were added to GBM cells to study its effect on viability, invasion, relocation, apoptosis, and mRNA and protein levels. Hence, we explored the antiproliferative, anti-invasive, and apoptotic activity of SA on GBM human U-251 cells.

Results: MTT assay and live/dead assay revealed the anti-proliferative activity of SA on U-251 glioma cells. Apoptotic activity of SA was shown by DAPI staining, caspase-3, Bax, and Bcl-2 mRNA expressions. The cell cycle regulation was also confirmed by reducing the mRNA expression of cyclinD1, CDK4, and CDK6. Treatment of SA with U-251 cells suppressed MMPs expressions and enhanced TIMPs protein levels.

Conclusion: Our findings put forward that SA could prevent GBM cells’ invasion and relocation. SA is an ideal neuroprotective agent for controlling brain malignancy.

Keywords: Glioblastoma, syringic acid, proliferation, invasion, apoptosis, cell toxicity.

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