Rhubarb Peony Decoction Downregulates the Expression of c-Fos after Intestinal
Epithelial Cells Hypoxia-reoxygenation Injury

Zhen-guo      Xu; Xi-qi      Chen; Yun-jie      Zhang; Yong      Zhu; Xiao-Shuang      Zhang; Meng      Wang; Yong-kun      Zhou

doi:10.2174/1386207325666220512140827

Abstract

Background: Intestinal ischemia-reperfusion (I/R) injury occurs in several clinical situations and after intestinal transplantation. This study aimed to examine the role of rhubarb peony decoction (RPD) in intestinal I/R injury.

Methods: Different concentrations of RPD were set to treat IEC-6 and Caco-2 cells. Cell proliferation and apoptosis were measured by CCK-8 and flow cytometry assays. High-throughput transcriptome sequencing was performed on IEC-6 cells treated with hypoxia-reoxygenation (HR) or HR and RPD.

Results: RPD treatment significantly promoted the proliferation of IEC-6 and Caco-2 cells and inhibited apoptosis. Sequencing results identified 109 significantly up-regulated genes and 36 significantly down-regulated genes in the RPD group. In addition, the results of western blot suggested that HR induced the expression of c-Fos, and the treatment of RPD prevented the HR-induced c- Fos expression. Importantly, knockdown of c-Fos rescued the HR-inhibited cell proliferation and HR-induced apoptosis.

Conclusions: In conclusion, RPD was beneficial in protecting the survival of intestinal epithelial cells under HR stress. Furthermore, the increase in c-Fos expression after HR stress was closely related to the proliferation and apoptosis of intestinal epithelial cells.

Keywords: Intestinal ischemia-reperfusion, rhubarb peony decoction, c-Fos, proliferation, apoptosis, thrombolysis.

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DOI https://dx.doi.org/10.2174/1386207325666220512140827	Print ISSN 1386-2073
Publisher Name Bentham Science Publisher	Online ISSN 1875-5402

Combinatorial Chemistry & High Throughput Screening

Rhubarb Peony Decoction Downregulates the Expression of c-Fos after Intestinal Epithelial Cells Hypoxia-reoxygenation Injury

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