Abstract
Objective: This study used network pharmacology and molecular docking technology to explore the molecular mechanism of Panax notoginseng in the treatment of disseminated intravascular coagulation.
Methods: The main active components and targets of Panax notoginseng were screened by the TCMSP database, and DIC-related targets were obtained from the GeneCards database. PPI network was constructed by String and Cytoscape, GO gene analysis and KEGG pathway enrichment analysis were performed by DAVID, and molecular docking was performed by AutoDock software.
Results: Eight active compounds and 51 potential therapeutic targets of Sanqi were screened. The key targets include VEGF, MAPK3, EGFR, STAT3 and so on. Beta-sitosterol, Stigmasterol, quercetin, DFV, and Diop were identified aspotential candidate ingredients. There are 95 KEGG enrichment pathways. The metabolic pathways involving a large number of genes mainly include the PI3K-Akt signaling pathway, Rap1 signaling pathway, VEGF signaling pathway and TNF signaling pathway.
Conclusion: This study revealed the multi-component, multi-target and multi-channel action characteristics of Sanqi in the treatment of DIC and predicted the possible pharmacodynamic substances, key targets and action pathways, which provided a theoretical basis for its new drug development and mechanism of action.
Keywords: Sanqi, disseminated intravascular coagulation, network pharmacology, molecular docking, panax notoginseng, pharmacodynamic.
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