Bioactive Compounds from Mimosa pudica Leaves Extract with Their α-
glucosidase and Protein Tyrosine Phosphatase 1B Inhibitory Activities in
vitro and in silico Approaches

Pham   Thi   Lan; Nguyen   Thi   Thu; Vu   Thi   Thom; Nguyen   Thi Hai   Yen; Phan   Hong   Minh; Bui   Son   Nhat; Nguyen   Thi   Huyen; Nguyen   Hong   Nhung; Ta Thi   Thu   Hang; Pham   Thi Nguyet   Hang; Bui   Thanh   Tung

doi:10.2174/1570180819666220510105202

Abstract

Background: Mimosa pudica Linn has been used in traditional medicine to support the treatment of type 2 diabetes. In the present study, we aimed to isolate and evaluate α-glucosidase and Protein Tyrosine Phosphatase 1B (PTP1B) inhibitory activities of bioactive compounds from Mimosa pudica’s leaf extract.

Methods: Mimosa pudica leaves were extracted with 80% of ethanol. Bioactive compounds were isolated using a column chromatographic technique and elucidated the structure based on the nuclear magnetic resonance and electrospray ionization mass spectrometry spectral data. The α- glucosidase and PTP1B inhibitory activities of the isolated compounds were evaluated using pnitrophenyl phosphate and p-nitrophenyl-α-D-glucopyranoside as a substrate, respectively. Molecular docking and molecular dynamics are used to study the interaction between isolated compounds and proteins. Lipinski’s rule of five was used to evaluate the drug-like properties of isolated compounds. Predict pharmacokinetic parameters were evaluated using the pkCSM tool.

Results: Protocatechuic acid and syringic acid were isolated and identified using spectroscopic methods. Protocatechuic acid and syringic acid considerably inhibited α-glucosidase enzyme at IC₅₀ values of 416.17 ± 9.41 μM and 490.78 ± 9.28 μM, respectively. Furthermore, protocatechuic acid and syringic acid expressed strong PTP1B inhibitory activity at IC₅₀ values of 248.83 ± 7.66 μM and 450.31 ± 7.77 μM, respectively. Molecular docking and molecular dynamics results showed the interactions of protocatechuic acid and syringic acid with amino acids of PTP1B and α-glucosidase enzyme. Lipinski’s rule of five and absorption, distribution, metabolism, excretion, and toxicity studies predicted that protocatechuic acid and syringic acid have drug-likeness properties. In molecular docking simulation, protocatechuic acid and syringic acid gave relatively negative free binding energies and interacted with many amino acids in the active sites of PTP1B and α-glucosidase. The molecular dynamics simulation results of the complexes were also relatively stable.

Conclusion: Our results showed that protocatechuic and syringic acids could be promising compounds for type 2 diabetes treatment.

Keywords: Mimosa pudica, α-glucosidase, protein tyrosine phosphatase 1B, Protocatechuic acid, Syringic acid

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DOI https://dx.doi.org/10.2174/1570180819666220510105202	Print ISSN 1570-1808
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Bioactive Compounds from Mimosa pudica Leaves Extract with Their α- glucosidase and Protein Tyrosine Phosphatase 1B Inhibitory Activities in vitro and in silico Approaches

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