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Current Nanomedicine

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ISSN (Online): 2468-1881

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Research Article

Ultrasmall Superparamagnetic Iron Oxide Nanoparticles Synthesized by Micellar Approach as a Potential Dual-Mode T1-T2 Contrast Agent

Author(s): Watson Beck, Laudemir C. Varanda*, Simone J.S. Lopes, Daniel A. Moraes, Natalia M. Santos and Maria Eduarda S. D. Lino

Volume 12, Issue 1, 2022

Published on: 14 June, 2022

Page: [63 - 75] Pages: 13

DOI: 10.2174/2468187312666220509213555

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Abstract

Objective: A micellar approach is used to synthesize Ultrasmall Superparamagnetic Iron Oxide Nanoparticles (USPIONs) with an average diameter of 3.4±0.5 nm, suitable for dual-mode T1-T2 contrast agents.

Methods: Micelles with 3.8 nm, measured by dynamic light scattering, were obtained by selforganizing the surfactant iron(III) dodecyl sulfate (IDS) in 1-octanol. IDS was prepared by replacing Na+ cation in sodium dodecyl sulfate molecule, and its critical micelle concentration (CMC) was measured by electrical conductivity. The USPIONs were synthesized in a biphasic system: IDS in octanol (55% above the CMC) and water containing NaBH4.

Results: A yellow precipitate is immediately formed at the water/alcohol interface, rapidly changes to a black one, and transfers to the aqueous phase. The magnetite phase was confirmed by X-ray diffraction and Mössbauer spectroscopy. The magnetic behavior shows a major paramagnetic character with a weak ferromagnetic component at 5 K, the latter attributed to the interparticle couplings below its blocking temperature (TB = 35 K). The particles were coated with carboxymethyl dextran, showing an isoelectric point of 2.7 with electrokinetic potential around -30 mV in the physiological pH range. Magnetic relaxation measurements showed relaxivity values r1 = 0.17 mM-1 s-1 and r2 = 1.73 mM-1 s-1 (r2/r1 = 10) in a 3T field. These values infer that the ultrasmall size affects the interactions with the protons of the nearby water molecules. The r2 value decreases because the core magnetization decreases with size; r1 intensifies due to the high surface.

Conclusion: The results show a system with high colloidal stability, non-cytotoxic, and potential application as T1-T2 dual-mode contrast agents.

Keywords: Micellar system, iron(III) dodecyl sulfate, cation-substituted surfactant, USPIONs, biocompatibility, colloidal stability, dual-mode contrast agents.

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