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Current Proteomics

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ISSN (Print): 1570-1646
ISSN (Online): 1875-6247

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Short Communication

Proteomics and Computational Analysis of Cytosolic Proteome of a Thermoacidophilic Euryarchaeon, Picrophilus torridus

Author(s): Neelja Singhal, Anjali Garg, Nirpendra Singh, Manish Kumar and Manisha Goel*

Volume 19, Issue 4, 2022

Published on: 01 August, 2022

Page: [290 - 298] Pages: 9

DOI: 10.2174/1570164619666220429121247

Price: $65

Abstract

Background: Picrophilus torridus is a thermoacidophilic archaeon that thrives in an extremely low pH (0-1) and high temperatures (50-60°C). Thus, it is a suitable organism to study microbial genetics and metabolic adaptations to the extremely acidic and moderate thermal environment.

Objective: In the present study we have conducted a global proteome analysis of P. torridus and discerned the cytosolic proteome of P. torridus using gel-free, liquid chromatographymass spectrometry (LC-MS/MS).

Methods: The cytosolic proteins of P. torridus were extracted and identified using gel-free, LCMS/ MS. Gene Ontology-based pathway analysis and protein-protein interaction studies were performed to understand the role of various cytosolic proteins in sustaining the thermoacidophilic environment. Also, domain analysis of hypothetical/uncharacterized proteins was performed.

Results: Using gel-free LC-MS/MS, 408 cytosolic proteins of P. torridus were identified, including 36 hypothetical/uncharacterized proteins. Thus, we could identify 26.58 % of the theoretical proteome of P. torridus. The majority of the cytosolic proteins were observed to be multi-functional and involved in activities related to microbial metabolism.

Conclusion: Comparison with an earlier study that used gel-based LC-MS analysis to identify cytosolic proteins of P. torridus revealed that gel-free LC-MS was better in identifying more number of proteins and also, higher/lower molecular weight proteins. The findings of this study may contribute to our understanding of the P. torridus proteome and serve as a foundation for future proteomic research on other thermoacidophilic archaea.

Keywords: Thermoacidophilic, liquid chromatography-mass spectrometry, cytosolic proteins, archaea, proteome, computational analysis.

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