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Research Article

香芹酚对代谢相关肝脂肪变性和内皮功能障碍体外模型的有益作用：脂肪酸在干扰香芹酚与血清白蛋白结合中的作用

卷 29, 期 30, 2022

发表于: 06 June, 2022

页: [5113 - 5129] 页: 17

弟呕挨: 10.2174/0929867329666220401103643

价格: $65

摘要

背景：香芹酚是一种植物酚类单萜烯，主要用作食品添加剂和植物化学物质。 目的：利用肝脂肪变性和内皮功能障碍的细胞模型，评估香芹酚在体外的降脂和保护作用。我们还研究了香芹酚与白蛋白（血液中小化合物的生理转运蛋白）的结合是否以及如何因高水平脂肪酸（FAs）的存在而改变。 方法：用外源性FA处理的肝脏FaO细胞模拟肝细胞血脂病;暴露于过氧化氢的内皮HECV细胞是内皮功能障碍的模型。在这些模型中，我们分光光度法测量了卡瓦特罗处理前后的脂质积累和释放，脂过氧化，自由基产生和一氧化氮释放。通过吸收和发射光谱评估在存在或不存在高水平FA的情况下与白蛋白的香芹酚结合。 结果：香芹酚抵消了肝细胞中的脂质积累和氧化应激，并保护内皮细胞免受氧化应激和功能障碍的影响。此外，高水平的FA减少了香芹酚与白蛋白的结合。 结论：卡瓦特罗在体外改善肝内皮细胞功能障碍方面具有良好的潜力。高水平的循环FA可能与香芹酚竞争与白蛋白的结合，从而影响其运输和生物分布。

关键词: 香芹酚，非酒精性脂肪性肝病（NAFLD），内皮功能障碍，长链脂肪酸，血清白蛋白，HECV。

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