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Current Enzyme Inhibition

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ISSN (Print): 1573-4080
ISSN (Online): 1875-6662

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Research Article

Alpha-glucosidase and Alpha-amylase Inhibition Activity of Avocado Fruit of Nepalese Origin

Author(s): Kamal Dhakal, Dipesh Shrestha, Tamlal Pokhrel, Devi Prasad Bhandari and Achyut Adhikari*

Volume 18, Issue 2, 2022

Published on: 13 May, 2022

Page: [105 - 109] Pages: 5

DOI: 10.2174/1573408018666220324110300

Price: $65

Abstract

Background: Diabetes has become a major health problem due to its high prevalence, morbidity, and mortality rate. Reducing postprandial hyperglycemia has become the main target in the treatment of diabetes mellitus. So, developing new drugs with fewer side effects has been a major priority.

Objective: The main objective of this study is to investigate total phenolic and flavonoid content, antioxidant activity, α-glucosidase, and α-amylase inhibition activity of Persea Americana Mill (avocado) pulp and seed.

Methods: The α-glucosidase and α-amylase inhibition activity were performed using substrates PNPG and CNPG3, respectively. DPPH free radical scavenging assay was used to perform the antioxidant activity. The total phenolic content was estimated using folin-ciocalu’s reagent. Likewise, the aluminium trichloride method was applied to find out the total flavonoid content.

Results: The crude methanolic extract of avocado seed revealed potent α-glucosidase inhibition activity with an IC50 1.959±0.93μg/mL followed by the avocado pulp 308±2.36μg/mL. Similarly, the IC50 for the α-amylase inhibition activity of avocado seed was found to be 120.3±1.382μg/mL. In addition, the avocado pulp and seed revealed a significant antioxidant activity with IC50 values of 75.01±0.72μg/mL and 6.445±0.62μg/mL, respectively, compared to the standard quercetin 1.525±0.5μg/mL. The total phenolic content of avocado pulp and the seed was reported as 7.031±2.87 mg of GAE/g, and 142.96±1.589 mg of GAE/g, respectively. Similarly, the total flavonoid content of avocado pulp and the seed was found to be 6.313±1.301 mg of QE/g and 48.696±0.110 mg of GAE/g, respectively.

Conclusion: The avocado seed of Nepali origin was found to inhibit the digestive enzyme significantly. These findings indicate that avocado fruit of Nepali origin has the potential to develop as an alternative food therapy for diabetic patients. Further research is required to find out the inhibitor compounds.

Keywords: Diabetes mellitus, α-glucosidase, α-amylase, antioxidant, Persea americana, avocado.

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[1]
O’Donnell C. Diabetes Contact Lens Practice. Elsevier: Amsterdam 2018; pp. 314-20.
[http://dx.doi.org/10.1016/B978-0-7020-6660-3.00034-4]
[2]
Yin Z, Zhang W, Feng F, Zhang Y, Kang W. α-Glucosidase inhibitors isolated from medicinal plants. Food Sci Hum Wellness 2014; 3(3–4): 136-74.
[http://dx.doi.org/10.1016/j.fshw.2014.11.003]
[3]
Martinez-Gonzalez AI, Díaz-Sánchez ÁG, Rosa LA, Vargas-Requena CL, Bustos-Jaimes I, Alvarez-Parrilla AE. Polyphenolic compounds and digestive enzymes: In vitro non-covalent interactions. Molecules 2017; 22(4): E669.
[http://dx.doi.org/10.3390/molecules22040669] [PMID: 28441731]
[4]
Tomasik P, Horton D. Chapter 2 - Enzymatic conversions of starch. In: Horton DBT-A in CC and B, editorAcademic Press 2012; pp. 59-436. Available from: https://www.sciencedirect.com/science/article/pii/B9780123965233000014
[5]
Mikawlrawng K. Chapter 18 - Aspergillus in biomedical research. In: New and Future Developments in Microbial Biotechnology and Bioengineering , Gupta VK, Eds Elsevier Amsterdam 2016; pp. 229-42. Available from: https://www.sciencedirect.com/science/article/pii/B9780444635051000191
[6]
Khoo CM. Diabetes mellitus treatment. In: International Encyclopedia of Public Health. Second Edition. Academic Press: Cambridge 2017; pp. 288-93.
[http://dx.doi.org/10.1016/B978-0-12-803678-5.00108-9]
[7]
Shrestha D, Sharma P, Adhikari A, Mandal AK, Verma A. A Review on nepalese medicinal plants used traditionally as alpha-amylase and alpha-glucosidase inhibitors against diabetes mellitus. Curr Tradit Med 2021; 7(5): 63-72.
[http://dx.doi.org/10.2174/2215083807666210702144103]
[8]
Krentz AJ, Sinclair AJ. The evolution of glucose-lowering drugs for type 2 diabetes. In: Nutritional and Therapeutic Interventions For Diabetes and Metabolic Syndrome. Elsevier Inc.: Amsterdam 2012; pp. 459-74.
[http://dx.doi.org/10.1016/B978-0-12-385083-6.00036-X]
[9]
Rosa MM, Dias T. Commonly used endocrine drugs Handbook of Clinical Neurology. Elsevier B.V. : Amsterdam 2014; pp. 809-24.
[http://dx.doi.org/10.1093/med/9780199929146.003.0002]
[10]
Amit Koparde A, Chandrashekar Doijad R, Shripal Magdum C. Natural products in drug discovery. In: Pharmacognosy - Medicinal Plants IntechOpen. 2019. Available from: www.intechopen.com
[http://dx.doi.org/10.5772/intechopen.82860]
[11]
Sarker SD, Latif Z, Gray AI. Natural Product Isolation. In: Natural Products Isolation. Humana Press 2006; pp. 1-25.
[http://dx.doi.org/10.1385/1-59259-955-9:1]
[12]
Xu L, Li Y, Dai Y, Peng J. Natural products for the treatment of type 2 diabetes mellitus: Pharmacology and mechanisms. Pharmacol Res 2018; 130: 451-65.
[http://dx.doi.org/10.1016/j.phrs.2018.01.015] [PMID: 29395440]
[13]
Shapiro K, Gong WC. Natural products used for diabetes. J Am Pharm Assoc 2002; 42(2): 217-6.
[http://dx.doi.org/10.1331/108658002763508515]
[14]
Zaid H, Mahdi AA, Tamrakar AK, Saad B, Razzaque MS, Dasgupta A. Natural active ingredients for diabetes and metabolism disorders treatment. Evidence-based Complementary and Alternative Medicine 2016 2016.
[http://dx.doi.org/10.1155/2016/2965214]
[15]
Jimenez P, Garcia P, Quitral V, et al. Pulp, leaf, peel and seed of avocado fruit: A review of bioactive compounds and healthy benefits. Food Rev Int 2021; 37(6): 619-55.
[http://dx.doi.org/10.1080/87559129.2020.1717520]
[16]
Owolabi MA, Jaja SI, Coker HAB. Vasorelaxant action of aqueous extract of the leaves of Persea americana on isolated thoracic rat aorta. Fitoterapia 2005; 76(6): 567-73.
[http://dx.doi.org/10.1016/j.fitote.2005.04.020] [PMID: 15990249]
[17]
Tcheghebe T, Nyamen LD, Tatong F, Seukep AJ. Ethnobotanical uses, phytochemical and pharmacological profiles, and toxicity of Persea americana mill.: An overview. Pharmacologyonline 2016; 3: 213-21.
[18]
Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem 2003; 83(4): 547-50.
[http://dx.doi.org/10.1016/S0308-8146(03)00151-1]
[19]
Chang C-C, Yang M-H, Wen H-M, Chern J-C. Estimation of total flavonoid content in propolis by two complementary colometric methods. Yao Wu Shi Pin Fen Xi 2020; 10(3): 3.
[http://dx.doi.org/10.38212/2224-6614.2748]
[20]
Mensor LL, Menezes FS, Leitão GG, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001; 15(2): 127-30.
[http://dx.doi.org/10.1002/ptr.687] [PMID: 11268111]
[21]
Abbas G, Al-Harrasi AS, Hussain H. α-Glucosidase enzyme inhibitors from natural products. Discovery and Development of Antidiabetic Agents from Natural Products: Natural Product Drug Discovery . Elsevier Inc. 2017; pp. 251-69.
[http://dx.doi.org/10.1016/B978-0-12-809450-1.00009-0]
[22]
Senger MR, Gomes LC, Ferreira SB, Kaiser CR, Ferreira VF, Silva FP Jr. Kinetics studies on the inhibition mechanism of pancreatic α-amylase by glycoconjugated 1H-1,2,3-triazoles: A new class of inhibitors with hypoglycemiant activity. ChemBioChem 2012; 13(11): 1584-93.
[http://dx.doi.org/10.1002/cbic.201200272] [PMID: 22753086]
[23]
Majeed M, Majeed S, Mundkur L, et al. Standardized Emblica officinalis fruit extract inhibited the activities of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 and displayed antioxidant potential. J Sci Food Agric 2020; 100(2): 509-16.
[http://dx.doi.org/10.1002/jsfa.10020] [PMID: 31487036]
[24]
Isaac A, Ganiyu O, Akinyemi AJ, Ajani RA, Bakare O, Olanrewaju O. Avocado pear fruits and leaves aqueous extracts inhibit α-amylase, α-glucosidase and Snp induced lipid peroxidation- an insight into mechanisms involve in management of type 2 diabetes. Int J Appl Nat Sci 2014; 3(5): 21-34.
[25]
Putra IMWA, Sukesi KA, Sulistyadewi NPE. Antioxidant Capacity and α-Amilase Inhibition by Avocado (Persea americana Mill) Peel and Red Ginger (Zingiber officinale var. Rubrum) based Functional Drink. Acta Chim Asiana 2020; 3(1): 135-42.
[http://dx.doi.org/10.29303/aca.v3i1.22]
[26]
Gondwe M, Kamadyaapa DR, Tufts MA, Chuturgoon AA, Ojewole JAO, Musabayane CT. Effects of Persea americana Mill (Lauraceae) [“Avocado”] ethanolic leaf extract on blood glucose and kidney function in streptozotocin-induced diabetic rats and on kidney cell lines of the proximal (LLCPK1) and distal tubules (MDBK). Methods Find Exp Clin Pharmacol 2008; 30(1): 25-35.
[http://dx.doi.org/10.1358/mf.2008.30.1.1147769] [PMID: 18389095]
[27]
Alarcon-Aguilara FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber CC, Flores-Saenz JL. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol 1998; 61(2): 101-10.
[http://dx.doi.org/10.1016/S0378-8741(98)00020-8] [PMID: 9683340]
[28]
Oboh G, Odubanjo VO, Bello F, et al. Aqueous extracts of avocado pear (Persea americana Mill.) leaves and seeds exhibit anti-cholinesterases and antioxidant activities in vitro. J Basic Clin Physiol Pharmacol 2016; 27(2): 131-40.
[http://dx.doi.org/10.1515/jbcpp-2015-0049] [PMID: 26812783]
[29]
Lara-Flores AA, Araújo RG, Rodríguez-Jasso RM, Aguedo M, Aguilar CN, Trajano HL, et al. Bioeconomy and biorefinery: valorization of hemicellulose from lignocellulosic biomass and potential use of avocado residues as a promising resource of bioproducts. In: Energy, Environment, and Sustainability . Springer Nature 2018; pp. 141-70.

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