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Current Aging Science

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ISSN (Print): 1874-6098
ISSN (Online): 1874-6128

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Research Article

Serum Klotho Concentrations in Young and Older Men During Prolonged Exercise in Temperate and Hot Conditions

Author(s): Kelli E. King, James J. McCormick, Sean R. Notley, Naoto Fujii and Glen P. Kenny*

Volume 15, Issue 2, 2022

Published on: 12 April, 2022

Page: [180 - 185] Pages: 6

DOI: 10.2174/1874609815666220304200939

Price: $65

Abstract

Background: The protein klotho protects cellular function during various physiological stressors, such as exercise, however it is unknown how the age-related decline in klotho production affects responses during exercise, especially in the heat.

Objective: Our objective was to determine the effect of exercise in temperate and hot environmental conditions on serum klotho concentrations in young and older men.

Methods: 12 young (mean ± SD: 22 ± 3 years) and 12 older (59 ± 4 years) men performed 180 minutes of moderate-intensity treadmill walking (metabolic rate: 200 W/m2) in a temperate (wetbulb globe temperature [WBGT] 16°C, achieved with 21.9°C, 35% relative humidity [RH]) and hot (WBGT 32°C, achieved with 41.4°C, 35% RH) environment. Serum klotho was assessed before and after exercise, as well as 60-min post-exercise recovery in the respective environments.

Results: Absolute klotho concentrations were greater in young versus older men during all measured time points in the temperate (p = 0.032), but not the hot condition (p = 0.064). In the hot condition, the change in serum klotho from baseline was significantly higher after exercise in the heat (mean ± SEM: +251 ± 73 pg/mL) than the temperate (+75 ± 57 pg/mL) environment in both groups (p = 0.026). However, this elevation was not maintained during recovery.

Conclusion: We showed that prolonged exercise in a temperate environment does not elicit a klotho response in either group. In contrast, despite lower resting klotho levels, the older men showed a similar exercise-induced increase in serum klotho response as their younger counterparts.

Keywords: heat stress, exercise, aging, klotho, serum, metabolic stress.

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[1]
Kuro-o M, Matsumura Y, Aizawa H, et al. Mutation of the mouse klotho gene leads to a syndrome resembling ageing. Nature 1997; 390(6655): 45-51.
[http://dx.doi.org/10.1038/36285] [PMID: 9363890]
[2]
Amaro-Gahete FJ. de-la-O A, Jurado-Fasoli L, Ruiz JR, Cas-tillo MJ, Gutiérrez Á. Role of exercise on S-klotho protein regulation: A systematic review. Curr Aging Sci 2018; 11(2): 100-7.
[http://dx.doi.org/10.2174/1874609811666180702101338] [PMID: 29962352]
[3]
Witkowski JM. Soroczyńska-Cybula M, Bryl E, Smoleń ska Z, Jóź wik A. Klotho--a common link in physiological and rheu-matoid arthritis-related aging of human CD4+ lymphocytes. J Immunol 2007; 178(2): 771-7.
[http://dx.doi.org/10.4049/jimmunol.178.2.771] [PMID: 17202338]
[4]
Hu MC, Shiizaki K, Kuro-o M, Moe OW. Fibroblast growth factor 23 and Klotho: Physiology and pathophysiology of an endocrine network of mineral metabolism. Annu Rev Physiol 2013; 75(1): 503-33.
[http://dx.doi.org/10.1146/annurev-physiol-030212-183727] [PMID: 23398153]
[5]
Imura A, Iwano A, Tohyama O, et al. Secreted Klotho protein in sera and CSF: Implication for post-translational cleavage in release of Klotho protein from cell membrane. FEBS Lett 2004; 565(1-3): 143-7.
[http://dx.doi.org/10.1016/j.febslet.2004.03.090] [PMID: 15135068]
[6]
Razzaque MS. The role of Klotho in energy metabolism. Nat Rev Endocrinol 2012; 8(10): 579-87.
[http://dx.doi.org/10.1038/nrendo.2012.75] [PMID: 22641000]
[7]
Carracedo J, Buendía P, Merino A, et al. Klotho modulates the stress response in human senescent endothelial cells. Mech Ageing Dev 2012; 133(11-12): 647-54.
[http://dx.doi.org/10.1016/j.mad.2012.09.002] [PMID: 23000105]
[8]
Maekawa Y, Ishikawa K, Yasuda O, et al. Klotho suppresses TNF-alpha-induced expression of adhesion molecules in the endothelium and attenuates NF-kappaB activation. Endocrine 2009; 35(3): 341-6.
[http://dx.doi.org/10.1007/s12020-009-9181-3] [PMID: 19367378]
[9]
Kenny GP, Wilson TE, Flouris AD, Fujii N. Heat exhaustion. Handb Clin Neurol 2018; 157: 505-29.
[http://dx.doi.org/10.1016/B978-0-444-64074-1.00031-8] [PMID: 30459023]
[10]
Avin KG, Coen PM, Huang W, et al. Skeletal muscle as a regulator of the longevity protein, Klotho. Front Physiol 2014; 5: 189.
[http://dx.doi.org/10.3389/fphys.2014.00189] [PMID: 24987372]
[11]
Rao Z, Zheng L, Huang H, Feng Y, Shi R. α-Klotho expression in mouse tissues following acute exhaustive exercise Front Physiol 2019; 10: 1498.
[http://dx.doi.org/10.3389/fphys.2019.01498] [PMID: 31920703]
[12]
Santos-Dias A, MacKenzie B, Oliveira-Junior MC, Moyses RM, Consolim-Colombo FM, Vieira RP. Longevity protein klotho is induced by a single bout of exercise. Br J Sports Med 2017; 51(6): 549-50.
[http://dx.doi.org/10.1136/bjsports-2016-096139] [PMID: 27251899]
[13]
Lovlin R, Cottle W, Pyke I, Kavanagh M, Belcastro AN. Are indices of free radical damage related to exercise intensity. Eur J Appl Physiol Occup Physiol 1987; 56(3): 313-6.
[http://dx.doi.org/10.1007/BF00690898] [PMID: 3569239]
[14]
King MA, Clanton TL, Laitano O. Hyperthermia, dehydration, and osmotic stress: Unconventional sources of exercise-induced reactive oxygen species. Am J Physiol Regul Integr Comp Physiol 2016; 310(2): R105-14.
[http://dx.doi.org/10.1152/ajpregu.00395.2015] [PMID: 26561649]
[15]
Rhind SG, Gannon GA, Shephard RJ, Buguet A, Shek PN, Radomski MW. Cytokine induction during exertional hyper-thermia is abolished by core temperature clamping: Neuroen-docrine regulatory mechanisms. Int J Hyperthermia 2004; 20(5): 503-16.
[http://dx.doi.org/10.1080/02656730410001670651] [PMID: 15277023]
[16]
Yamazaki Y, Imura A, Urakawa I, et al. Establishment of sandwich ELISA for soluble alpha-Klotho measurement: Age-dependent change of soluble alpha-Klotho levels in healthy subjects. Biochem Biophys Res Commun 2010; 398(3): 513-8.
[http://dx.doi.org/10.1016/j.bbrc.2010.06.110] [PMID: 20599764]
[17]
Amaro-Gahete FJ, De-la-O A, Jurado-Fasoli L, Ruiz JR, Cas-tillo MJ. Association of basal metabolic rate and fuel oxida-tion in basal conditions and during exercise, with plasma S-klotho: The FIT-AGEING study. Aging (Albany NY) 2019; 11(15): 5319-33.
[http://dx.doi.org/10.18632/aging.102100] [PMID: 31390595]
[18]
Haak JL, Buettner GR, Spitz DR, Kregel KC. Aging augments mitochondrial susceptibility to heat stress. Am J Physiol Regul Integr Comp Physiol 2009; 296(3): R812-20.
[http://dx.doi.org/10.1152/ajpregu.90708.2008] [PMID: 19144753]
[19]
Wright HE, McLellan TM, Larose J, Hardcastle SG, Boulay P, Kenny GP. Are circulating cytokine responses to exercise in the heat augmented in older men? Appl Physiol Nutr Metab 2014; 39(2): 117-23.
[http://dx.doi.org/10.1139/apnm-2013-0223] [PMID: 24476465]
[20]
Kenny GP, Groeller H, McGinn R, Flouris AD. Age, human performance, and physical employment standards. Appl Physiol Nutr Metab 2016; 41(6)(Suppl. 2): S92-S107.
[http://dx.doi.org/10.1139/apnm-2015-0483] [PMID: 27277571]
[21]
Kenny GP, Vierula M, Maté J, Beaulieu F, Hardcastle SG, Reardon F. A field evaluation of the physiological demands of miners in Canada’s deep mechanized mines. J Occup Environ Hyg 2012; 9(8): 491-501.
[http://dx.doi.org/10.1080/15459624.2012.693880] [PMID: 22715930]
[22]
Meade RD, Lauzon M, Poirier MP, Flouris AD, Kenny GP. The physical demands of electrical utilities work in North America. J Occup Environ Hyg 2016; 13(1): 60-70.
[http://dx.doi.org/10.1080/15459624.2015.1077966] [PMID: 26317802]
[23]
Notley SR, Akerman AP, Friesen BJ, et al. Heat tolerance and occupational heat exposure limits in older men with and with-out type 2 diabetes or hypertension. Med Sci Sports Exerc 2021; 53(10): 2196-206.
[http://dx.doi.org/10.1249/MSS.0000000000002698] [PMID: 33988544]
[24]
CSEP Certified fitness appraiser resource manual Glouces-ter. Ontario: Canadian Society for Exercise Physiology 1986.
[25]
Larose J, Boulay P, Sigal RJ, Wright HE, Kenny GP. Age-related decrements in heat dissipation during physical activity occur as early as the age of 40. PLoS One 2013; 8(12): e83148.
[http://dx.doi.org/10.1371/journal.pone.0083148] [PMID: 24349447]
[26]
Kuro-o M. Klotho as a regulator of oxidative stress and se-nescence. Biol Chem 2008; 389(3): 233-41.
[http://dx.doi.org/10.1515/BC.2008.028] [PMID: 18177265]
[27]
Starkie RL, Hargreaves M, Rolland J, Febbraio MA. Heat stress, cytokines, and the immune response to exercise. Brain Behav Immun 2005; 19(5): 404-12.
[http://dx.doi.org/10.1016/j.bbi.2005.03.005] [PMID: 16061150]
[28]
Slimen IB, Najar T, Ghram A, Dabbebi H, Ben Mrad M, Abdrabbah M. Reactive oxygen species, heat stress and oxi-dative-induced mitochondrial damage. A review. Int J Hyperthermia 2014; 30(7): 513-23.
[http://dx.doi.org/10.3109/02656736.2014.971446] [PMID: 25354680]
[29]
Kuro-o M. A potential link between phosphate and aging--lessons from Klotho-deficient mice. Mech Ageing Dev 2010; 131(4): 270-5.
[http://dx.doi.org/10.1016/j.mad.2010.02.008] [PMID: 20197072]
[30]
Crasto CL, Semba RD, Sun K, Cappola AR, Bandinelli S, Ferrucci L. Relationship of low-circulating “anti-aging” klotho hormone with disability in activities of daily living among older community-dwelling adults. Rejuvenation Res 2012; 15(3): 295-301.
[http://dx.doi.org/10.1089/rej.2011.1268] [PMID: 22530731]
[31]
Ji N, Luan J, Hu F, et al. Aerobic exercise-stimulated Klotho upregulation extends life span by attenuating the excess pro-duction of reactive oxygen species in the brain and kidney. Exp Ther Med 2018; 16(4): 3511-7.
[http://dx.doi.org/10.3892/etm.2018.6597] [PMID: 30233703]
[32]
Zhu L, Stein LR, Kim D, et al. Klotho controls the brain-immune system interface in the choroid plexus. Proc Natl Acad Sci USA 2018; 115(48): E11388-96.
[http://dx.doi.org/10.1073/pnas.1808609115] [PMID: 30413620]
[33]
D’Souza AW, Notley SR, Kenny GP. The relation between age and sex on whole-body heat loss during exercise-heat stress. Med Sci Sports Exerc 2020; 52(10): 2242-9.
[http://dx.doi.org/10.1249/MSS.0000000000002373] [PMID: 32496737]

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