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ISSN (Print): 2211-5501
ISSN (Online): 2211-551X

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Research Article

Detection of Phage and Prophage Expression in Multidrug-Resistant Salmonella Serovars Isolated from Retail Meats Using mRNA-Seq

Author(s): Thuy Thi Bich Vo*, Viet Thanh Nguyen, Cuong Xuan Hoang, Duc Hieu Nguyen, Duc Minh Nguyen, Tram Bao Nguyen and Minh Ngoc Nghiem

Volume 11, Issue 1, 2022

Published on: 10 May, 2022

Page: [32 - 38] Pages: 7

DOI: 10.2174/2211550111666220304102952

Price: $65

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Abstract

Background: Salmonella serovars contamination of food items shows a negative effect on the food industry and causes a great hazard for the consumers. Although developed hygiene methods, Salmonella serovars are still a high risk to human health, and new methods for the identification of Salmonella spp. are needed.

Objective: Bacteriophages have been considered a good method for detecting bacteria owing to their high target cell specificity, simplicity and cheapness. Bacteriophages can be used for biocontrol of Salmonella spp. without the cultural methods. Moreover, phages or phage-derived proteins can also be used to detect quickly and specifically bacteria in food.

Methods: This study provides bacteriophage expression in six multidrug-resistant Salmonella isolated from retail meats in Hanoi, Vietnam using mRNA sequencing.

Results: Our results showed that diverse bacteriophages are naturally present in retail meats (chicken, beef, and pork meat), and phage Salmon_118970_sal3_NC_031940 is the most common phage in Salmonella on the retail meats.

Conclusion: These results provide useful information for developing a new method to detect Salmonella serovars effectively. Further surveillance programs and research are a necessity to limit the spread of multidrug-resistant Salmonella spp.

Keywords: Salmonella, phage, prophage, retail meats, bacteria detection, multidrug-resistant Salmonella , mRNASeq.

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[1]
Fernandes E, Martins VC, Nóbrega C, et al. A bacteriophage detection tool for viability assessment of Salmonella cells. Biosens Bioelectron 2014; 52: 239-46.
[http://dx.doi.org/10.1016/j.bios.2013.08.053]
[2]
Schmelcher M, Loessner MJ. Application of bacteriophages for detection of foodborne pathogens. Bacteriophage 2014; 4(1): e28137-7.
[http://dx.doi.org/10.4161/bact.28137] [PMID: 24533229]
[3]
Hendrix RW, Smith MC, Burns RN, Ford ME, Hatfull GF. Evolutionary relationships among diverse bacteriophages and prophages: All the world’s a phage. Proc Natl Acad Sci USA 1999; 96(5): 2192-7.
[http://dx.doi.org/10.1073/pnas.96.5.2192] [PMID: 10051617]
[4]
Park HJ, Kim HJ, Park SH, Shin EG, Kim JH, Kim HY. Direct and quantitative analysis of Salmonella enterica serovar Typhimurium using real-time PCR from artificially contaminated chicken meat. J Microbiol Biotechnol 2008; 18(8): 1453-8.
[PMID: 18756108]
[5]
Myint MS, Johnson YJ, Tablante NL, Heckert RA. The effect of pre-enrichment protocol on the sensitivity and specificity of PCR for detection of naturally contaminated Salmonella in raw poultry compared to conventional culture. Food Microbiol 2006; 23(6): 599-604.
[http://dx.doi.org/10.1016/j.fm.2005.09.002] [PMID: 16943057]
[6]
Kanki M, Sakata J, Taguchi M, et al. Effect of sample preparation and bacterial concentration on Salmonella enterica detection in poultry meat using culture methods and PCR assaying of preenrichment broths. Food Microbiol 2009; 26(1): 1-3.
[http://dx.doi.org/10.1016/j.fm.2008.07.010] [PMID: 19028296]
[7]
Nghiem MN, Nguyen VT, Nguyen TTH, Nguyen TD, Vo TTB. Antimicrobial resistance gene expression associated with multidrug re-sistant Salmonella spp. isolated from retail meat in Hanoi, Vietnam. Int Microbiol 2017; 20(2): 85-93.
[PMID: 28617526]
[8]
Kearse M, Moir R, Wilson A, et al. Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 2012; 28(12): 1647-9.
[http://dx.doi.org/10.1093/bioinformatics/bts199] [PMID: 22543367]
[9]
Wattam AR, Abraham D, Dalay O, et al. PATRIC, the bacterial bioinformatics database and analysis resource. Nucleic Acids Res 2014; 42(Database issue): D581-91.
[http://dx.doi.org/10.1093/nar/gkt1099] [PMID: 24225323]
[10]
Arndt D, Grant JR, Marcu A, et al. PHASTER: A better, faster version of the PHAST phage search tool. Nucleic Acids Res 2016; 44(W1): W16-21.
[http://dx.doi.org/10.1093/nar/gkw387] [PMID: 27141966]
[11]
Laura MK, La Donna P. Bacteriophages. StatPearls Publishing LLC: Treasure Island. 2018.
[12]
Casjens SR. Comparative genomics and evolution of the tailed-bacteriophages. Curr Opin Microbiol 2005; 8(4): 451-8.
[http://dx.doi.org/10.1016/j.mib.2005.06.014] [PMID: 16019256]
[13]
Hatfull GF. Bacteriophage genomics. Curr Opin Microbiol 2008; 11(5): 447-53.
[http://dx.doi.org/10.1016/j.mib.2008.09.004] [PMID: 18824125]
[14]
Morris P, Marinelli LJ, Jacobs-Sera D, Hendrix RW, Hatfull GF. Genomic characterization of mycobacteriophage Giles: Evidence for phage acquisition of host DNA by illegitimate recombination. J Bacteriol 2008; 190(6): 2172-82.
[http://dx.doi.org/10.1128/JB.01657-07] [PMID: 18178732]
[15]
Clokie MR, Millard AD, Letarov AV, Heaphy S. Phages in nature. Bacteriophage 2011; 1(1): 31-45.
[http://dx.doi.org/10.4161/bact.1.1.14942] [PMID: 21687533]
[16]
Nanduri V, Sorokulova IB, Samoylov AM, Simonian AL, Petrenko VA, Vodyanoy V. Phage as a molecular recognition element in biosen-sors immobilized by physical adsorption. Biosens Bioelectron 2007; 22(6): 986-92.
[http://dx.doi.org/10.1016/j.bios.2006.03.025] [PMID: 16730970]
[17]
Kuhn JC. Detection of Salmonella by bacteriophage Felix 01. Methods Mol Biol 2007; 394: 21-37.
[18]
Fortier LC, Sekulovic O. Importance of prophages to evolution and virulence of bacterial pathogens. Virulence 2013; 4(5): 354-65.
[http://dx.doi.org/10.4161/viru.24498] [PMID: 23611873]
[19]
León M, Bastías R. Virulence reduction in bacteriophage resistant bacteria. Front Microbiol 2015; 6: 343.
[http://dx.doi.org/10.3389/fmicb.2015.00343]

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