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Current Stem Cell Research & Therapy

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ISSN (Print): 1574-888X
ISSN (Online): 2212-3946

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Research Article

Angiogenic and Migratory Gene Expression Analysis of Stem Cells From Exfoliated Deciduous Teeth for Wound Repair Application

Author(s): Nur Syazwani Aziz, Ahmad Azlina and Norhayati Yusop*

Volume 17, Issue 5, 2022

Published on: 18 April, 2022

Page: [466 - 479] Pages: 14

DOI: 10.2174/1574888X17666220221142524

Price: $65

Abstract

Background: The migration and differentiation of stem cells take place during the reparative phase of the healing cascade. Chemokine ligands and receptors are the key players in the homing process during the early stage of capillary morphogenesis. Stem cells from exfoliated deciduous teeth are known to possess a huge potential benefit for tissue regeneration. However, the gene expression of SHED engaging in angiogenesis and migratory activity during tissue healing is not fully understood. This study aims to assess the gene expression of SHED following in-vitro angiogenesis and migratory induction protocol.

Methods: Scratch test assay was conducted following an angiogenic induction of SHED by supplementation of EGM-2 and VEGF. For the detection of migratory cell markers, angiogenic markers, and stem cell markers, RNA samples were extracted on days 1, 3, 7, 10, and 14 after the angiogenic induction in a transwell chamber, followed by RT-PCR analysis.

Results: The findings suggested that SHED formed endothelial cells at higher capacity under an immature state with higher seeding density. SHED undergoing angiogenesis and migratory activity showed elevated IL-8, CCR1, CXCR4, and CCL28 expression. CCR1 expression significantly increased in the A+M+ group (p<0.05).

Conclusion: The gene expression of these chemokines, particularly CCR1, which closely represent cellular migration, suggests the potential use of SHED for cell-based therapy to enhance tissue repair.

Keywords: Cell biology, stem cell. wound repair, cell migration, angiogenesis, gene expression.

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Graphical Abstract

[1]
Fatimah SS, Tan GC, Chua K, Fariha MMN, Tan AE, Hayati AR. Stemness and angiogenic gene expression changes of se-rial-passage human amnion mesenchymal cells. Microvasc Res 2013; 86: 21-9.
[http://dx.doi.org/10.1016/j.mvr.2012.12.004 ] [PMID: 23261754]
[2]
Li L, He Y, Zhao M, Jiang J. Collective cell migration: Impli-cations for wound healing and cancer invasion. Burns Trauma 2013; 1(1): 21-6.
[http://dx.doi.org/10.4103/2321-3868.113331 ] [PMID: 27574618]
[3]
Grada A, Otero-Vinas M, Prieto-Castrillo F, Obagi Z, Falanga V. Research techniques made simple: Analysis of collective cell migration using the wound healing assay. J Invest Dermatol 2017; 137(2): e11-6.
[http://dx.doi.org/10.1016/j.jid.2016.11.020 ] [PMID: 28110712]
[4]
Lee K-B, Kim D-I. Clinical application of stem cells for thera-peutic angiogenesis in patients with peripheral arterial disease. Int J Stem Cells 2009; 2(1): 11-7.
[http://dx.doi.org/10.15283/ijsc.2009.2.1.11 ] [PMID: 24855515]
[5]
Yamaza T, Kentaro A, Chen C, et al. Immunomodulatory properties of stem cells from human exfoliated deciduous teeth. Stem Cell Res Ther 2010; 1(1): 5.
[http://dx.doi.org/10.1186/scrt5 ] [PMID: 20504286]
[6]
Bento LW, Zhang Z, Imai A, et al. Endothelial differentiation of SHED requires MEK1/ERK signaling. J Dent Res 2013; 92(1): 51-7.
[http://dx.doi.org/10.1177/0022034512466263 ] [PMID: 23114032]
[7]
Hashim SNM, Yusof MFH, Chandra H, et al. Human amniotic membrane as a matrix for endothelial differentiation of VEGF-treated dental stem cells. Cell Mol Bioeng 2019; 12: 599-613.
[8]
Cordeiro MM, Dong Z, Kaneko T, et al. Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod 2008; 34(8): 962-9.
[http://dx.doi.org/10.1016/j.joen.2008.04.009 ] [PMID: 18634928]
[9]
Dellian M, Witwer BP, Salehi HA, Yuan F, Jain RK. Quantita-tion and physiological characterization of angiogenic vessels in mice: Effect of basic fibroblast growth factor, vascular en-dothelial growth factor/vascular permeability factor, and host microenvironment. Am J Pathol 1996; 149(1): 59-71.
[PMID: 8686763]
[10]
Nikolić N, Krstić A, Trivanović D, et al. Mesenchymal stem cell properties of dental pulp cells from deciduous teeth. Arch Biol Sci 2011; 63(4): 933-42.
[http://dx.doi.org/10.2298/ABS1104933N]
[11]
Doan CC, Le TL, Hoang NS, Doan NT, Le VD, Do MS. Dif-ferentiation of umbilical cord lining membrane-derived mes-enchymal stem cells into endothelial-like cells. Iran Biomed J 2014; 18(2): 67-75.
[PMID: 24518546]
[12]
Bentley K, Chakravartula S. The temporal basis of angiogenesis. Philos Trans R Soc Lond B Biol Sci 1720; 372(1720): 20150522.
[http://dx.doi.org/10.1098/rstb.2015.0522]
[13]
Skóra JP, Płonek T, Barć P, et al. The formation of blood vessel after the administration of the plasmid encoding Ang-1 gene in Fischer rats. Adv Clin Exp Med 2016; 25(4): 611-5.
[http://dx.doi.org/10.17219/acem/62430 ] [PMID: 27629833]
[14]
Cao J, Ehling M, März S, et al. Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis. Nat Commun 2017; 8(1): 2210.
[http://dx.doi.org/10.1038/s41467-017-02373-8 ] [PMID: 29263363]
[15]
Byrne AM, Bouchier-Hayes DJ, Harmey JH. Angiogenic and cell survival functions of vascular endothelial growth factor (VEGF). J Cell Mol Med 2005; 9(4): 777-94.
[http://dx.doi.org/10.1111/j.1582-4934.2005.tb00379.x ] [PMID: 16364190]
[16]
Baek SJ, Kang SK, Ra JC. In vitro migration capacity of hu-man adipose tissue-derived mesenchymal stem cells reflects their expression of receptors for chemokines and growth fac-tors. Exp Mol Med 2011; 43(10): 596-603.
[http://dx.doi.org/10.3858/emm.2011.43.10.069 ] [PMID: 21847008]
[17]
Akpinar G, Kasap M, Aksoy A, Duruksu G, Gacar G, Karaoz E. Phenotypic and proteomic characteristics of human dental pulp derived mesenchymal stem cells from a natal, an exfoli-ated deciduous, and an impacted third molar tooth. Stem Cells Int 2014; 2014: 457059.
[http://dx.doi.org/10.1155/2014/457059]
[18]
Siddiqui AJ, Blomberg P, Wärdell E, et al. Combination of angiopoietin-1 and vascular endothelial growth factor gene therapy enhances arteriogenesis in the ischemic myocardium. Biochem Biophys Res Commun 2003; 310(3): 1002-9.
[http://dx.doi.org/10.1016/j.bbrc.2003.09.111 ] [PMID: 14550304]
[19]
Koblizek TI, Weiss C, Yancopoulos GD, Deutsch U, Risau W. Angiopoietin-1 induces sprouting angiogenesis in vitro. Curr Biol 1998; 8(9): 529-32.
[http://dx.doi.org/10.1016/S0960-9822(98)70205-2 ] [PMID: 9560344]
[20]
Abdel-Malak NA, Mofarrahi M, Mayaki D, Khachigian LM, Hussain SN. Early growth response-1 regulates angiopoietin-1-induced endothelial cell proliferation, migration, and differ-entiation. Arterioscler Thromb Vasc Biol 2009; 29(2): 209-16.
[http://dx.doi.org/10.1161/ATVBAHA.108.181073 ] [PMID: 19112164]
[21]
Koch AE, Polverini PJ, Kunkel SL, et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 1992; 258(5089): 1798-801.
[http://dx.doi.org/10.1126/science.1281554 ] [PMID: 1281554]
[22]
Ju L, Zhou Z, Jiang B, Lou Y, Guo X. Autocrine VEGF and IL-8 promote migration via SRC/VAV2/RAC1/PAK1 signaling in human umbilical vein endothelial cells. Cell Physiol Biochem 2017; 41(4): 1346-59.
[http://dx.doi.org/10.1159/000465389 ] [PMID: 28278510]
[23]
Harris ES, Nelson WJ. VE-cadherin: At the front, center, and sides of endothelial cell organization and function. Curr Opin Cell Biol 2010; 22(5): 651-8.
[http://dx.doi.org/10.1016/j.ceb.2010.07.006 ] [PMID: 20708398]
[24]
Nikolova-Krstevski V, Bhasin M, Otu HH, Libermann T, Oettgen P. Gene expression analysis of embryonic stem cells expressing VE-cadherin (CD144) during endothelial differen-tiation. BMC Genomics 2008; 9(1): 240.
[http://dx.doi.org/10.1186/1471-2164-9-240 ] [PMID: 18498633]
[25]
Kiran MS, Viji RI, Kumar SV, Prabhakaran AA, Sudhakaran PR. Changes in expression of VE-cadherin and MMPs in en-dothelial cells: Implications for angiogenesis. Vasc Cell 2011; 3(1): 6.
[http://dx.doi.org/10.1186/2045-824X-3-6 ] [PMID: 21349163]
[26]
Guo M, Breslin JW, Wu MH, Gottardi CJ, Yuan SY. VE-cadherin and β-catenin binding dynamics during histamine-induced endothelial hyperpermeability. Am J Physiol Cell Physiol 2008; 294(4): C977-84.
[http://dx.doi.org/10.1152/ajpcell.90607.2007 ] [PMID: 18287330]
[27]
Fathi F, Rezabakhsh A, Rahbarghazi R, Rashidi M-R. Early-stage detection of VE-cadherin during endothelial differentia-tion of human mesenchymal stem cells using SPR biosensor. Biosens Bioelectron 2017; 96: 358-66.
[http://dx.doi.org/10.1016/j.bios.2017.05.018 ] [PMID: 28527412]
[28]
Hwang J, Kim CW, Son KN, et al. Angiogenic activity of hu-man CC chemokine CCL15 in vitro and in vivo. FEBS Lett 2004; 570(1-3): 47-51.
[http://dx.doi.org/10.1016/j.febslet.2004.06.023 ] [PMID: 15251437]
[29]
Hwang J, Son K-N, Kim CW, et al. Human CC chemokine CCL23, a ligand for CCR1, induces endothelial cell migration and promotes angiogenesis. Cytokine 2005; 30(5): 254-63.
[http://dx.doi.org/10.1016/j.cyto.2005.01.018 ] [PMID: 15927850]
[30]
Bousquenaud M, Schwartz C, Léonard F, Rolland-Turner M, Wagner D, Devaux Y. Monocyte chemotactic protein 3 is a homing factor for circulating angiogenic cells. Cardiovasc Res 2012; 94(3): 519-25.
[http://dx.doi.org/10.1093/cvr/cvs140 ] [PMID: 22492674]
[31]
Huang Z, Ma T, Ren PG, Smith RL, Goodman SB. Effects of orthopedic polymer particles on chemotaxis of macrophages and mesenchymal stem cells. J Biomed Mater Res A 2010; 94(4): 1264-9.
[http://dx.doi.org/10.1002/jbm.a.32803 ] [PMID: 20694994]
[32]
Kim J-H, Kim G-H, Kim J-W, et al. In vivo angiogenic capaci-ty of stem cells from human exfoliated deciduous teeth with human umbilical vein endothelial cells. Mol Cells 2016; 39(11): 790-6.
[http://dx.doi.org/10.14348/molcells.2016.0131 ] [PMID: 27871176]
[33]
Yu Q, Liu L, Lin J, et al. SDF-1α/CXCR4 axis mediates the migration of mesenchymal stem cells to the hypoxic-ischemic brain lesion in a rat model. Cell J 2015; 16(4): 440-7.
[PMID: 25685734]
[34]
Koellensperger E, Gramley F, Preisner F, Leimer U, Germann G, Dexheimer V. Alterations of gene expression and protein synthesis in co-cultured adipose tissue-derived stem cells and squamous cell-carcinoma cells: Consequences for clinical ap-plications. Stem Cell Res Ther 2014; 5(3): 65.
[http://dx.doi.org/10.1186/scrt454 ] [PMID: 24887580]
[35]
Huang G, Tao L, Shen S, Chen L. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells. Sci Rep 2016; 6: 27152.
[http://dx.doi.org/10.1038/srep27152 ] [PMID: 27250766]
[36]
Pan J, Kunkel EJ, Gosslar U, et al. A novel chemokine ligand for CCR10 and CCR3 expressed by epithelial cells in mucosal tissues. J Immunol 2000; 165(6): 2943-9.
[http://dx.doi.org/10.4049/jimmunol.165.6.2943 ] [PMID: 10975800]
[37]
Hattori Y, Kim H, Tsuboi N, et al. Therapeutic potential of stem cells from human exfoliated deciduous teeth in models of acute kidney injury. PLoS One 2015; 10(10): e0140121.
[http://dx.doi.org/10.1371/journal.pone.0140121 ] [PMID: 26509261]
[38]
Yamaza T, Alatas FS, Yuniartha R, et al. In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice. Stem Cell Res Ther 2015; 6(1): 171.
[http://dx.doi.org/10.1186/s13287-015-0154-6 ] [PMID: 26358689]

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