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Current Drug Therapy

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ISSN (Print): 1574-8855
ISSN (Online): 2212-3903

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Research Article

Investigating the Effects of Aqueous and Alcoholic Extracts of Allium hirtifolium and Allium jesdianum on Keratinase Activity of Trichophyton Mentagrophytes

Author(s): Maryam Sarlak, Zahra Nasiri, Mahboobeh Madani*, Kahin Shahanipour, Elena Tognoni and Pegah Shakib*

Volume 17, Issue 1, 2022

Published on: 27 December, 2021

Page: [39 - 44] Pages: 6

DOI: 10.2174/1574885517666211216160301

Price: $65

Abstract

Background: Trichophyton spp., as pathogenic species to humans and animals, cause different forms of dermatophytosis through the production of particular enzymes, playing an essential role in tissue invasion. Among these, herein, keratinase was investigated, for the specific case of Trichophyton mentagrophytes, as a target of the effects of Allium hirtifolium and Allium jesdianum extracts, thus pharmacological potential of these plants was studied against keratinase activity.

Methodology: Sampling was carried out on 20 bald patients from medical diagnostic laboratories and mycology centers, with suspected dermatophytosis of scalp. For confirming the presence of Trichophyton mentagrophytes in the specimens, different laboratory procedures were applied. Trichophyton mentagrophytes isolates were cultured on a screening medium containing keratin to verify production of the keratinase enzyme. The best enzyme-producing isolate was selected by measuring diameter of transparent halo around colony to be used in subsequent stages. Afterwards, the optimized conditions maximizing enzyme production and activity were determined. Finally, the inhibitory effect of different dilutions of aqueous and alcoholic extracts of Allium jesdianum and Allium hirtifolium on extracellular keratinase activity was studied.

Results: Sixteen out of 20 fungal isolates were identified as the Trichophyton mentagrophytes. The most desirable reduction on keratinase activity was reported for dilution values of 50 and 100 mg/ml of both aqueous and ethanolic extracts of A.jesdianum, though much more significant decrease belonged to the latter, and for dilution values of 25 and 100 mg/ ml of both aqueous and ethanolic extracts of A.hirtifolium.

Conclusion: Concerning our results, it is suggested that paying special attention to these natural compounds for the treatment of dermatophytosis could be remarkably effective, considering significant production of keratinase observed in T. mentagrophytes, and they are beneficial, as they have no side effects and offer an alternative to currently available medications, which are under the restriction of drug resistance.

Keywords: Allium hirtifolium, Allium jesdianum, trichophyton mentagrophytes, keratinase activity, dermatophytosis, drug resistance.

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[1]
Badali H, Mohammadi R, Mashedi O, de Hoog GS, Meis JF. In vitro susceptibility patterns of clinically important Trichophyton and Epidermophyton species against nine antifungal drugs. Mycoses 2015; 58(5): 303-7.
[http://dx.doi.org/10.1111/myc.12315] [PMID: 25757042]
[2]
Carrillo-Muñoz AJ, Tur-Tur C, Cárdenes DC, Estivill D, Giusiano G. Sertaconazole nitrate shows fungicidal and fungistatic activities against trichophyton rubrum, trichophyton mentagrophytes, and epidermophyton floccosum, causative agents of tinea pedis. Antimicrob Agents Chemother 2011; 55(9): 4420-1.
[http://dx.doi.org/10.1128/AAC.00219-11] [PMID: 21746955]
[3]
Premrov-Bajuk B, Zdovc I, Smrekar V, Križaj I, Leonardi A, Drobnič-Košorok M. Dermatophyte trichophyton mentagrophytes produces cysteine protease inhibitor. Acta Chim Slov 2011; 58(1): 33-40.
[PMID: 24061940]
[4]
Singh CJ. Characterization of an extracellular keratinase of Trichophyton simii and its role in keratin degradation. Mycopathologia 1997; 137(1): 13-6.
[http://dx.doi.org/10.1023/A:1006844201399] [PMID: 9299753]
[5]
Zhu J-x, Hu S-s, Gao S-q. Study on the extracellular enzymes activities of Microsporum canis. Zhongguo Pifu Xingbingxue Zazhi 2004; 39(5): 463-8.
[6]
Viani FC, Cazares Viani PR, Gutierrez Rivera IN, Gonçalves da Silva E, Rodrigues Paula C, Gambale W. Extracellular proteolytic activity and molecular analysis of Microsporum canis strains isolated from symptomatic and asymptomatic cats. Rev Iberoam Micol 2007; 24(1): 19-23.
[http://dx.doi.org/10.1016/S1130-1406(07)70004-9] [PMID: 17592886]
[7]
Hegazy MF, Matsuda H, Nakamura S, Hussein TA, Yoshikawa M, Paré PW. Chemical constituents and their antibacterial and antifungal activity from the Egyptian herbal medicine Chiliadenus montanus. Phytochemistry 2014; 103: 154-61.
[http://dx.doi.org/10.1016/j.phytochem.2014.03.027] [PMID: 24746418]
[8]
Shahidi Bonjar G, Aghighi S, Karimi Nik A. Antibacterial and antifungal survey in plants used in indigenous herbal-medicine of south east regions of Iran. J Biol Sci 2004; 4(3): 405-12.
[http://dx.doi.org/10.3923/jbs.2004.405.412]
[9]
Robles-Martínez M, González JFC, Pérez-Vázquez FJ, Montejano-Carrizales JM, Pérez E, Patiño-Herrera R. Antimycotic activity potentiation of Allium sativum extract and silver nanoparticles against Trichophyton rubrum. Chem Biodivers 2019; 16(4): e1800525.
[http://dx.doi.org/10.1002/cbdv.201800525] [PMID: 30614195]
[10]
Lanzotti V, Scala F, Bonanomi G. Compounds from Allium species with cytotoxic and antimicrobial activity. Phytochem Rev 2014; 13(4): 769-91.
[http://dx.doi.org/10.1007/s11101-014-9366-0]
[11]
Parvu M, Parvu AE, Roşca-Casian O, Vlase L, Groza G. Antifungal activity of Allium obliquum. J Med Plants Res 2010; 4(2): 138-41.
[12]
Amiri H. Chemical composition and antibacterial activity of the essential oil of Allium jesdianum Boiss. & buhse from Iran. J Med Plants 2007; 6: 39-44.
[13]
Ebrahimi R, Zamani Z, Kashi A. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Sci Hortic (Amsterdam) 2009; 119(4): 345-51.
[http://dx.doi.org/10.1016/j.scienta.2008.08.032]
[14]
Miri SM, Roughani A, Eds. Allium species growing in Iran: Chemical compositions and pharmacological activity. Proceedings of the first national congress and international fair of medicinal plants and strategies for persian medicine that affect diabetes.
[15]
Bhat R. Bioactive compounds of allium species. In: Bioactive Compounds in Underutilized Vegetables and Legumes. Springer 2020; pp. 1-20.
[16]
Ates A, Ozcan K, Ilkit M. Diagnostic value of morphological, physiological and biochemical tests in distinguishing Trichophyton rubrum from Trichophyton mentagrophytes complex. Med Mycol 2008; 46(8): 811-22.
[http://dx.doi.org/10.1080/13693780802108458] [PMID: 18608891]
[17]
Wawrzkiewicz K, Wolski T, Lobarzewski J. Screening the keratinolytic activity of dermatophytes in vitro. Mycopathologia 1991; 114(1): 1-8.
[http://dx.doi.org/10.1007/BF00436684] [PMID: 1713301]
[18]
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72(1-2): 248-54.
[http://dx.doi.org/10.1016/0003-2697(76)90527-3] [PMID: 942051]
[19]
Panahi J, Havasian MR. In vitro evaluation of the effect of herbal medicine of western of Iran, Ilam on clinical and standard spices of Candida albicans (Disk Diffusion Method); A systematic review. Int J Pharm Sci Res 2015; 6(2): 262-4.
[20]
Yu RJ, Harmon SR, Blank F. Hair digestion by a keratinase of trichophyton mentagrophytes. J Invest Dermatol 1969; 53(2): 166-71.
[http://dx.doi.org/10.1038/jid.1969.125] [PMID: 4979675]
[21]
Khurana A, Sardana K, Chowdhary A. Antifungal resistance in dermatophytes: recent trends and therapeutic implications. Fungal Genet Biol 2019; 132: 103255.
[http://dx.doi.org/10.1016/j.fgb.2019.103255] [PMID: 31330295]
[22]
Ebert A, Monod M, Salamin K, et al. Alarming india-wide phenomenon of antifungal resistance in dermatophytes: a multicentre study. Mycoses 2020; 63(7): 717-28.
[http://dx.doi.org/10.1111/myc.13091] [PMID: 32301159]
[23]
Sharma A, Chandra S, Sharma M. Difference in keratinase activity of dermatophytes at different environmental conditions is an attribute of adaptation to parasitism. Mycoses 2012; 55(5): 410-5.
[http://dx.doi.org/10.1111/j.1439-0507.2011.02133.x] [PMID: 22032519]
[24]
Costa DCM, Vermelho AB, Almeida CA, et al. Inhibitory effect of linalool-rich essential oil from Lippia alba on the peptidase and keratinase activities of dermatophytes. J Enzyme Inhib Med Chem 2014; 29(1): 12-7.
[http://dx.doi.org/10.3109/14756366.2012.743537] [PMID: 23323991]
[25]
Amin M, Kapadnis B. Heat stable antimicrobial activity of Allium ascalonicum against bacteria and fungi. Indian J Exp Biol 2005; 43(8): 751-4.
[26]
Razafsha M, Shams GM. Inhibitory effects of aqueous onion and garlic extracts on growth and keratinase activity in Trichophyton mentagrophytes. Iranian Biomed J 2003; 7(3): 113-8.
[27]
Aala F, Yusuf UK, Khodav A, Jamal F. In vitro antifungal activity of allicin alone and in combination with two medications against six dermatophytic fungi. Afr J Microbiol Res 2010; 4(5): 380-5.
[28]
Khan MSA, Ahmad I. In vitro antifungal, anti-elastase and anti-keratinase activity of essential oils of Cinnamomum-, Syzygium- and Cymbopogon-species against Aspergillus fumigatus and Trichophyton rubrum. Phytomedicine 2011; 19(1): 48-55.
[http://dx.doi.org/10.1016/j.phymed.2011.07.005] [PMID: 21893402]
[29]
Jedelská J. Pharmaceutical value of onions (Allium L) and related species of Central Asia. Promotionsarbeit, Universität Marburg 2007.

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