Evaluation of Anti-lice Topical Lotion of Ozonated Olive Oil and Comparison
of its Effect with Permethrin Shampoo

Atoosa       Haghighizadeh; Seyedeh    Azadeh Fallah    Mortezanejad; Saba       Dadpour; Omid       Rajabi

doi:10.2174/1574887116666211202122132

Abstract

Background: Pediculosis is an infestation of lice affecting mostly children and spreads by direct contact with the hair of someone infected. Although topical application of a pediculicide such as permethrin shampoo is the most common cure for head lice, the addition of alternative treatments is necessary due to permethrin resistance and some safety concerns, including irritation, burning sensation, tingling, and breathing problems. Recent studies have indicated that ozone, which is an allotropic form of oxygen, may have anti-pediculosis effects.

Objective: This study focused on the evaluation of an ozonated olive oil topical lotion for the treatment of pediculosis and the comparison of its effects with permethrin shampoo.

Methods: 121 patients with proven head pediculosis were divided into two groups. They were treated with either topical ozonated olive oil lotion or permethrin shampoo for one week.

Results: It was shown that the ozonation process enormously improved the physico-chemical properties of olive oil. Specifically, acid and peroxide values increased 60 fold and 200 fold, respectively, while there was a sharp decline for the iodine index (81.8±1.28 to 0 g iodine per 100 g). Additionally, all patients who received the ozonated olive oil lotion were entirely cured after one week of treatment, while those who were treated with permethrin, required to receive more. Statistical analysis of the data also proved this claim.

Conclusion: The prepared lotion has shown significant effects in controlling head lice. Thus, it can be prescribed as an effective anti-pediculosis medicine which is biocompatible and has high potential.

Keywords: Pediculosis, ozonated olive oil, permethrin resistance, head lice, anti-pediculosis medicine, Permethrin.

« Previous Next »

Graphical Abstract

[1] 
Bragg BN, Simon LV. Simon, Pediculosis humanis (Lice, Capitis, Pubis).Treasure Island. Florida: StatPearls Publishing 2019.
[2] 
Guenther LC. Pediculosis and pthiriasis (lice infestation). 2015.
[3] 
Leulmi H, Diatta G, Sokhna C, Rolain J-M, Raoult D. Assessment of oral ivermectin versus shampoo in the treatment of pediculosis (head lice infestation) in rural areas of Sine-Saloum, Senegal. Int J Antimicrob Agents  2016; 48(6): 627-32.
[http://dx.doi.org/10.1016/j.ijantimicag.2016.07.014] [PMID: 27866866] 
[4] 
Burgess IF. Current treatments for Pediculosis capitis. Curr Opin Infect Dis  2009; 22(2): 131-6.
[http://dx.doi.org/10.1097/QCO.0b013e328322a019] [PMID: 19283911] 
[5] 
Mac-Mary S, Messikh R, Jeudy A, et al. Assessment of the efficacy and safety of a new treatment for head lice. Int Sch Res Not  2012; 2012.
[6] 
Hipolito RB, Mallorca FG, Zuniga-Macaraig ZO, Apolinario PC, Wheeler-Sherman J. Head lice infestation: Single drug versus combination therapy with one percent permethrin and trimethoprim/sulfamethoxazole. Pediatrics  2001; 107(3): E30.
[http://dx.doi.org/10.1542/peds.107.3.e30] [PMID: 11230611] 
[7] 
Lindsay SW, Peock S. Insecticides against headlice in Glasgow. J R Soc Health  1993; 113(4): 181-3.
[http://dx.doi.org/10.1177/146642409311300406] [PMID: 8410909] 
[8] 
Tanyuksel M, Araz RE, Albay A, Aycicek H. Prevalence and treatment of Pediculus humanus capitis with 1 % permethrin and 0.4 % d-phenothrin in Turkey. Acta Med (Hradec Kralove)  2003; 46(2): 73-5.
[http://dx.doi.org/10.14712/18059694.2019.9] [PMID: 12926604] 
[9] 
Andrews EB, Joseph MC, Magenheim MJ, Tilson HH, Doi PA, Schultz MW. Postmarketing surveillance study of permethrin creme rinse. Am J Public Health  1992; 82(6): 857-61.
[http://dx.doi.org/10.2105/AJPH.82.6.857] [PMID: 1375002] 
[10] 
Rossini C, Castillo L, González A. Plant extracts and their components as potential control agents against human head lice. Phytochem Rev  2008; 7: 51.
[http://dx.doi.org/10.1007/s11101-006-9026-0] 
[11] 
Stephens M. Controlling head lice. Patient Care  2000; 34: 99-107.
[12] 
Takano-Lee M, Edman JD, Mullens BA, Clark JM. Home remedies to control head lice: assessment of home remedies to control the human head louse, Pediculus humanus capitis (Anoplura: Pediculidae). J Pediatr Nurs  2004; 19(6): 393-8.
[http://dx.doi.org/10.1016/j.pedn.2004.11.002] [PMID: 15637580] 
[13] 
Heukelbach J, Canyon D, Speare R. The effect of natural products on head lice: In vitro tests and clinical evidence. J Pediatr Infect Dis  2007; 2: 67-76.
[http://dx.doi.org/10.1055/s-0035-1557021] 
[14] 
Degner M, Damaschke N, Ewald H, O’keeffe S, Lewis E. UV LED-based fiber coupled optical sensor for detection of ozone in the ppm and ppb range. SENSORS, 2009 IEEE  2009; 95-9.
[http://dx.doi.org/10.1109/ICSENS.2009.5398230] 
[15] 
Guzzon R, Nardin T, Micheletti O, Nicolini G, Larcher R. Antimicrobial activity of ozone. Effectiveness against the main wine spoilage microorganisms and evaluation of impact on simple phenols in wine. Aust J Grape Wine Res  2013; 19: 180-8.
[http://dx.doi.org/10.1111/ajgw.12018] 
[16] 
Lin Y-C, Wu S-C. Effects of ozone exposure on inactivation of intra- and extracellular enterovirus 71. Antiviral Res  2006; 70(3): 147-53.
[http://dx.doi.org/10.1016/j.antiviral.2005.12.007] [PMID: 16838411] 
[17] 
Latino J, Keyser S. Viral inactivation using ozone. US20050051497A1, 2005.
[18] 
Sunnen GV. Hepatitis C and ozone therapy. Ozone Med 2001.
[19] 
Ouf SA, Moussa TA, Abd-Elmegeed AM, Eltahlawy SR. Anti-fungal potential of ozone against some dermatophytes. Braz J Microbiol  2016; 47(3): 697-702.
[http://dx.doi.org/10.1016/j.bjm.2016.04.014] [PMID: 27287337] 
[20] 
Liou C-T, Wang J-S, Ooi H-K. Effect of ozone treatment on Eimeria colchici oocysts. J Parasitol  2002; 88(1): 159-62.
[http://dx.doi.org/10.1645/0022-3395(2002)088[0159:EOOTOE]2.0.CO;2] [PMID: 12053957] 
[21] 
Travagli V, Zanardi I, Valacchi G, Bocci V. Ozone and ozonated oils in skin diseases: A review. Mediators Inflamm  2010; 2010: 610418.
[http://dx.doi.org/10.1155/2010/610418] [PMID: 20671923] 
[22] 
Valacchi G, Fortino V, Bocci V. The dual action of ozone on the skin. Br J Dermatol  2005; 153(6): 1096-100.
[http://dx.doi.org/10.1111/j.1365-2133.2005.06939.x] [PMID: 16307642] 
[23] 
Sunnen GV. Ozone in medicine: overview and future directions. J Adv Med  1988; 1: 15974.
[24] 
Nagayoshi M, Fukuizumi T, Kitamura C, Yano J, Terashita M, Nishihara T. Efficacy of ozone on survival and permeability of oral microorganisms. Oral Microbiol Immunol  2004; 19(4): 240-6.
[http://dx.doi.org/10.1111/j.1399-302X.2004.00146.x] [PMID: 15209994] 
[25] 
Criegee R. Mechanism of ozonolysis. Angew Chem Int Ed Engl  1975; 14: 745-52.
[http://dx.doi.org/10.1002/anie.197507451] 
[26] 
Gupta RC, Kanwar G. Determination of iodine numbers of edible oils. Biochem Educ  1994; 22: 47.
[http://dx.doi.org/10.1016/0307-4412(94)90174-0] 
[27] 
Cirlini M, Caligiani A, Palla G, De Ascentiis A, Tortini P. Stability studies of ozonized sunflower oil and enriched cosmetics with a dedicated peroxide value determination. Ozone Sci Eng  2012; 34: 293-9.
[http://dx.doi.org/10.1080/01919512.2012.692992] 
[28] 
Bocci V. Preparation of Ozonated Water and Oil for the Topical Therapy-Ozone as a Drinking Water Disinfectant: Ozone Disinfection to Prevent Nosocomial Infections. In: Bocci V, Ed. Ozone, A new Medicinal drug.  Dordrecht: Springer 2010; pp. 9-16.
[http://dx.doi.org/10.1007/978-90-481-9234-2_3] 
[29] 
Rajabi O, Sazgarnia A, Abbasi F, Layegh P. The activity of ozonated olive oil against leishmania major promastigotes, Iran. J Basic Med Sci  2015; 18: 915.
[PMID: 26523224] 
[30] 
Guideline ICHHT. Stability testing of new drug substances and products, Q1A (R2). Curr Sep  2003; 4: 1-24.
[31] 
Ugazio E, Tullio V, Binello A, Tagliapietra S, Dosio F. Ozonated oils as antimicrobial systems in topical applications. their characterization, current applications, and advances in improved delivery techniques. Molecules  2020; 25(2): 334.
[http://dx.doi.org/10.3390/molecules25020334] [PMID: 31947580] 
[32] 
Sadowska J, Johansson B, Johannessen E, Friman R, Broniarz- Press L, Rosenholm JB. Characterization of ozonated vegetable oils by spectroscopic and chromatographic methods. Chem Phys Lipids  2008; 151(2): 85-91.
[http://dx.doi.org/10.1016/j.chemphyslip.2007.10.004] [PMID: 18023273] 
[33] 
Moureu S, Violleau F, Ali Haimoud-Lekhal D, Calmon A. Influence of storage temperature on the composition and the antibacterial activity of ozonized sunflower oil. Ozone Sci Eng  2016; 38(2): 143-9.
[http://dx.doi.org/10.1080/01919512.2015.1128319] 
[34] 
Kim HS, Noh SU, Han YW, et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci  2009; 24(3): 368-74.
[http://dx.doi.org/10.3346/jkms.2009.24.3.368] [PMID: 19543419] 
[35] 
Carata E, Tenuzzo BA, Dini L. Powerful properties of ozonated extra virgin olive oil. In: Herb Med. London: IntechOpen 2018.
[http://dx.doi.org/10.5772/intechopen.73211] 
[36] 
Díaz MF, Hernández R, Martínez G, et al. Comparative study of ozonized olive oil and ozonized sunflower oil. J Braz Chem Soc  2006; 17: 403-7.
[http://dx.doi.org/10.1590/S0103-50532006000200026] 
[37] 
Abu-Gharbieh E, Bayoumi FA, Ahmed NG. Alleviation of antioxidant defense system by ozonized olive oil in DNBS-induced colitis in rats. Mediators Inflamm  2014; 2014: 967205.
[http://dx.doi.org/10.1155/2014/967205] [PMID: 25276059] 
[38] 
Geweely NSI. Antifungal activity of ozonized olive oil (Oleozone). Int J Agric Biol  2006; 8(5): 671-8.
[39] 
Mohammadi Z, Shalavi S, Soltani MK, Asgary S. A review of the properties and applications of ozone in endodontics: an update. Iran Endod J  2013; 8(2): 40-3.
[PMID: 23717326] 
[40] 
Uzun H, Ibanoglu E. Oxidation kinetics of hazelnut oil treated with ozone. Grasas Aceites  2018; 68(4): 222.
[http://dx.doi.org/10.3989/gya.0664171] 
[41] 
Skalska K, Ledakowicz S, Perkowski J, Sencio B. Germicidal properties of ozonated sunflower oil. Ozone Sci Eng  2009; 31(3): 232-7.
[http://dx.doi.org/10.1080/01919510902838669] 
[42] 
Díaz Gómez MF, Téllez GM, Cruz MA, Mancheno RG. Chemical analysis of ozonized theobroma fat. J Am Oil Chem Soc  2006; 83(11): 943-6.
[http://dx.doi.org/10.1007/s11746-006-5050-x] 
[43] 
Toloza AC, Lucia A, Zerba E, Masuh H, Picollo MI. Interspecific hybridization of Eucalyptus as a potential tool to improve the bioactivity of essential oils against permethrin-resistant head lice from Argentina. Bioresour Technol  2008; 99(15): 7341-7.
[http://dx.doi.org/10.1016/j.biortech.2007.12.067] [PMID: 18261899] 
[44] 
Barker SC, Altman PM. A randomised, assessor blind, parallel group comparative efficacy trial of three products for the treatment of head lice in children--melaleuca oil and lavender oil, pyrethrins and piperonyl butoxide, and a “suffocation” product. BMC Dermatol  2010; 10: 6.
[http://dx.doi.org/10.1186/1471-5945-10-6] [PMID: 20727129] 
[45] 
Burkhart CG, Burkhart CN. Safety and efficacy of pediculicides for head lice. Expert Opin Drug Saf  2006; 5(1): 169-79.
[http://dx.doi.org/10.1517/14740338.5.1.169] [PMID: 16370965] 
[46] 
Greive KA, Barnes TM. The efficacy of Australian essential oils for the treatment of head lice infestation in children: A randomised controlled trial. Australas J Dermatol  2018; 59(2): e99-e105.
[http://dx.doi.org/10.1111/ajd.12626] [PMID: 28266704] 
[47] 
de Groot AC, Schmidt E. Tea tree oil: Contact allergy and chemical composition. Contact Dermat  2016; 75(3): 129-43.
[http://dx.doi.org/10.1111/cod.12591] [PMID: 27173437] 
[48] 
Burgess IF, Silverston P. Head lice. Clin Evid  2011; 2011: 1703-43.
[PMID: 21575285] 
[49] 
Elvis AM, Ekta JS. Ozone therapy: A clinical review. J Nat Sci Biol Med  2011; 2(1): 66-70.
[http://dx.doi.org/10.4103/0976-9668.82319] [PMID: 22470237] 
[50] 
Xiaoqi W. Emerging roles of ozone in skin diseases. Zhong Nan Da Xue Xue Bao Yi Xue Ban  2018; 43(2): 114-23.
[http://dx.doi.org/10.11817/j.issn.1672-7347.2018.02.002] 
[51] 
De las Cagigas T, Bestard V, Menéndez S, Gómez M, Eng L. Use of the ozonized oil on patients with vulvovaginitis. Proc 1st Iberolatinoamerican Congr Ozone Appl 1990.

Rights & Permissions Print Cite

Article Metrics

15

1

Journal Information

For Authors

For Editors

For Reviewers

Explore Articles

Open Access

Open Access Articles

For Visitors

DOI https://dx.doi.org/10.2174/1574887116666211202122132	Print ISSN 1574-8871
Publisher Name Bentham Science Publisher	Online ISSN 1876-1038

Reviews on Recent Clinical Trials

Evaluation of Anti-lice Topical Lotion of Ozonated Olive Oil and Comparison of its Effect with Permethrin Shampoo

Abstract Play Pause

Graphical Abstract

Related Journals

Abstract