Functionalized Liposomes Fabrication and Characterization with Erythrocruorin for a Potential Application as a Red Blood Cells Substitute

Title:Functionalized Liposomes Fabrication and Characterization with Erythrocruorin for a Potential Application as a Red Blood Cells Substitute

Volume: 17 Issue: 6

Author(s): Nadia García-Flores, César Rodríguez-Beas, Ramón Alfonso Iñiguez-Palomares, José Manuel Galván-Moroyoqui, Juan Manuel Martínez-Soto, Ricardo López-Esparza and Ericka Rodríguez-León*

Affiliation:

• Nanotechnology Graduate Program, Department of Physics, University of Sonora, Rosales and Transversal, 83000 Hermosillo, Sonora,Mexico

Keywords: Liposomes, erythrocruorin, red blood cell substitutes, Eisenia fetida, polyethylene glycol, lecithin.

Abstract:

Aims: Erythrocruorin is considered for a potential application as a Red Blood Cells Substitute.

Background: Nanostructured materials such as liposomes and polymeric materials have offered new alternatives that allow the design of systems as potential substitutes for red blood cells.

Objective: Functionalized liposomes fabrication and characterization.

Methods: The liposomes manufactured with egg yolk lecithin prepared by the extrusion method were functionalized with EfEc and stabilized with polyethylene glycol (PEG) by an absorption method.

Results: The nanoplatform has a hydrodynamic diameter of 452.4 ± 24.56 nm; this size provides the ability to avoid problems such as system extravasation, also presents stability (ζ-potential = -30. 06 ± 0.42 mV) and SEM micrographs show a semi-spherical morphology. Also, EfEc showed a peak of fluorescence emission at 449 nm, which allowed us to observe the protein's presence on the surface of the liposomes employing CLSM.

Conclusion: In this work, we report the fabrication and characterization of liposomes with EfEc and PEG on their surface. The nanoplatform exhibits physical-chemical characteristics that confer a potential application as a substitute for red blood cells.

Cite this article as:

García-Flores Nadia , Rodríguez-Beas César , Iñiguez-Palomares Alfonso Ramón, Galván-Moroyoqui Manuel José , Martínez-Soto Manuel Juan , López-Esparza Ricardo and Rodríguez-León Ericka *, Functionalized Liposomes Fabrication and Characterization with Erythrocruorin for a Potential Application as a Red Blood Cells Substitute, Current Nanoscience 2021; 17 (6) . https://dx.doi.org/10.2174/1573413717666210620132628