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Research Article

大鼠骨髓来源间充质干细胞作为细胞治疗临床前药物对单核细胞粒细胞分化的影响

卷 22, 期 2, 2022

发表于: 20 July, 2021

页: [152 - 161] 页: 10

弟呕挨: 10.2174/1566523221666210519111933

价格: $65

摘要

背景：骨髓单个核细胞 (BM-MNCs) 作为造血和间充质干细胞 (MSCs) 的集合，能够产生所有血细胞谱系。使用细胞因子、生长因子或能够分泌这些因子的细胞将有助于刺激这些细胞增殖和分化为成熟细胞系。另一方面，MSCs 是多能基质细胞，可以分化成各种细胞谱系。此外，这些细胞可以通过分泌细胞因子和生长因子来控制造血过程。本研究旨在通过流式细胞术分析评估细胞表面标志物，研究 BM 衍生的 MSCs 对 MNCs 分化的影响。 方法：为此目的，使用密度梯度离心从大鼠BM中纯化MNC。此后，它们被培养、扩展和表征。接下来，BM 衍生的 MSCs 与 MNCs 共培养，然后单独培养 MNCs（对照组）或与 BM 衍生的 MSCs 共培养的 MNCs（实验组）。最后，在第 7 天收集它们并进行流式细胞术分析以进行粒细胞标志物和 ERK 蛋白研究。 结果：与对照组相比，实验组CD34、CD16、CD11b、CD18粒细胞标志物的表达水平以及ERK蛋白表达水平明显升高。 结论：因此，可以得出结论，MSCs可以通过ERK蛋白的表达影响MNCs的粒细胞分化，ERK蛋白是ERK信号通路的关键组成部分。

关键词: 间充质干细胞、粒细胞分化、单核细胞、基于细胞的治疗、临床药剂、骨髓单核细胞

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