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当代肿瘤药物靶点

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ISSN (Print): 1568-0096
ISSN (Online): 1873-5576

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Research Article

MiR-129-5p 通过靶向 SOX4 和 RUNX1 提高 NSCLC 细胞的放射敏感性

卷 21, 期 8, 2021

发表于: 15 April, 2021

页: [702 - 712] 页: 11

弟呕挨: 10.2174/1568009621666210415094350

价格: $65

摘要

背景：microRNA (miRNA) 的失调在非小细胞肺癌 (NSCLC) 的放射敏感性中占有突出地位。 MiR-129-5p 可以阻断多种肿瘤的发展。然而，miR-129-5p 是否调节 NSCLC 细胞的放射敏感性仍然未知。 目的：本研究旨在探讨miR-129-5p在NSCLC放射敏感性中的作用及其机制。 方法：使用 A549 和 H1299 细胞构建抗辐射 NSCLC 细胞系（A549-R 和 H1299-R）。采用定量实时聚合酶链反应 (qRT-PCR) 来量化 miR-129-5p、SRY-box 转录因子 4 (SOX4) mRNA 和 RUNX 家族转录因子 1 (RUNX1) mRNA 的表达水平。流式细胞术检测细胞凋亡和细胞周期。使用细胞计数试剂盒-8 (CCK-8) 测定和集落形成实验来测量细胞增殖。通过蛋白质印迹检查γ-H2AX以确认DNA损伤。应用双荧光素酶报告基因实验来分析 miR-129-5p、RUNX1 和 SOX4 之间的相互作用。结果：在 A549-R 和 H1299-R 细胞中，与野生型细胞系相比，miR-129-5p 表达显着降低，而 SOX4 和 RUNX1 表达增加。将 miR-129-5p 转染到 NSCLC 细胞系中可显着诱导细胞凋亡、DNA 损伤、细胞周期停滞，并抑制细胞增殖和集落形成。 RUNX1和SOX4被证实为miR-129-5p的靶基因，RUNX1或SOX4的恢复可以抵消miR-129-5p对A549-R细胞的影响。 结论：MiR-129-5p 通过靶向 RUNX1 和 SOX4 使 A549-R 和 H1299-R 细胞对辐射敏感。

关键词: NSCLC、miR-129-5p、RUNX1、SOX4、放射敏感性、qTR-PCR。

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