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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Review Article

Functional Properties of Kefiran in the Medical Field and Food Industry

Author(s): Afsaneh Salari, Mohammad Hashemi and Asma Afshari*

Volume 23, Issue 3, 2022

Published on: 22 March, 2021

Page: [388 - 395] Pages: 8

DOI: 10.2174/1389201022666210322121420

Price: $65

Abstract

Kefir is produced through the fermentation of milk using kefir grain as a starter culture. Kefir grains include heterogeneous microorganisms embedded in a polysaccharide matrix called kefiran which is considered a biofilm; it also has many uses due to its therapeutic values. Kefiran is a microbial exopolysaccharide (EPS) obtained from the flora (acid-lactic bacteria and yeasts) of kefir grains and glucose units, in almost the same proportion. Kefiran has prebiotic nature agitating the growth of probiotics in the gastrointestinal tract of the human entity. It extends certain therapeutic benefits by balancing the microbiota in the intestine. This review presents the most recent advances regarding kefir and kefiran, their cultural condition, biological activities, and potential applications in the health and food industries.

Keywords: Kefir, kefiran, exopolysaccharide, prebiotic, medical field, food industry.

Graphical Abstract

[1]
Lopitz-Otsoa, F.; Rementeria, A.; Elguezabal, N.; Garaizar, J. Kefir: una comunidad simbiótica de bacterias y levaduras con propiedades saludables. Rev. Iberoam. Micol., 2006, 23(2), 67-74.
[http://dx.doi.org/10.1016/S1130-1406(06)70016-X] [PMID: 16854180]
[2]
Farnworth, E.R. The beneficial health effects of fermented foods-potential probiotics around the world. J. Nutraceut. Funct. Med. Foods, 2005, 4(3-4), 93-117.
[3]
Serafini, F.; Turroni, F.; Ruas-Madiedo, P.; Lugli, G.A.; Milani, C.; Duranti, S.; Zamboni, N.; Bottacini, F.; van Sinderen, D.; Margolles, A.; Ventura, M. Kefir fermented milk and kefiran promote growth of Bifidobacterium bifidum PRL2010 and modulate its gene expression. Int. J. Food Microbiol., 2014, 178, 50-59.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2014.02.024] [PMID: 24667318]
[4]
Guzel-Seydim, Z.B.; Kok-Tas, T.; Greene, A.K.; Seydim, A.C. Review: functional properties of kefir. Crit. Rev. Food Sci. Nutr., 2011, 51(3), 261-268.
[http://dx.doi.org/10.1080/10408390903579029] [PMID: 21390946]
[5]
Roginski, H.; Fuquay, J.W.; Fox, P.F. Encyclopedia of dairy sciences; Academic press, 2003, pp. 1-4.
[6]
Kalamaki, M.S.; Angelidis, A.S. Isolation and molecular identification of yeasts in Greek kefir. Int. J. Dairy Technol., 2017, 70(2), 261-268.
[http://dx.doi.org/10.1111/1471-0307.12329]
[7]
Arslan, S. A review: chemical, microbiological and nutritional characteristics of kefir. CYTA J. Food, 2015, 13(3), 340-345.
[http://dx.doi.org/10.1080/19476337.2014.981588]
[8]
Chryssanthopoulos, C.; Maridaki, M. Nutritional aspects of yogurt and functional dairy products; Development and manufacture of yogurt and other functional dairy products, 2010, pp. 267-305.,
[9]
Ghoddusi, H.; Özer, B. Microbiology and biochemistry of yogurt and other fermented milk products.Dairy Microbiology and Biochemistry; CRC Press: Boca Raton, FL, 2014, pp. 181-227.
[10]
Micheli, L.; Uccelletti, D.; Palleschi, C.; Crescenzi, V. Isolation and characterisation of a ropy Lactobacillus strain producing the exopolysaccharide kefiran. Appl. Microbiol. Biotechnol., 1999, 53(1), 69-74.
[http://dx.doi.org/10.1007/s002530051616] [PMID: 10645627]
[11]
Chen, H-C.; Wang, S-Y.; Chen, M-J. Microbiological study of lactic acid bacteria in kefir grains by culture-dependent and culture-independent methods. Food Microbiol., 2008, 25(3), 492-501.
[http://dx.doi.org/10.1016/j.fm.2008.01.003] [PMID: 18355674]
[12]
Hamet, M.F.; Piermaria, J.A.; Abraham, A.G. Selection of EPS-producing Lactobacillus strains isolated from kefir grains and rheological characterization of the fermented milks. Lebensm. Wiss. Technol., 2015, 63(1), 129-135.
[http://dx.doi.org/10.1016/j.lwt.2015.03.097]
[13]
Tamime, A.; Wszolek, M.; Božanić, R.; Özer, B. Popular ovine and caprine fermented milks. Small Rumin. Res., 2011, 101(1-3), 2-16.
[http://dx.doi.org/10.1016/j.smallrumres.2011.09.021]
[14]
de Oliveira Leite, A.M.; Miguel, M.A.L.; Peixoto, R.S.; Rosado, A.S.; Silva, J.T.; Paschoalin, V.M.F. Microbiological, technological and therapeutic properties of kefir: a natural probiotic beverage. Braz. J. Microbiol., 2013, 44(2), 341-349.
[http://dx.doi.org/10.1590/S1517-83822013000200001] [PMID: 24294220]
[15]
Kotova, I.; Cherdyntseva, T.; Netrusov, A. Russian kefir grains microbial composition and its changes during production process. Advances in microbiology, infectious diseases and public health; Springer, 2016, pp. 93-121.
[http://dx.doi.org/10.1007/5584_2016_2]
[16]
Witthuhn, R.; Schoeman, T.; Britz, T. Characterisation of the microbial population at different stages of kefir production and kefir grain mass cultivation. Int. Dairy J., 2005, 15(4), 383-389.
[http://dx.doi.org/10.1016/j.idairyj.2004.07.016]
[17]
Magalhães, K.T.; de Melo Pereira, G.V.; Campos, C.R.; Dragone, G.; Schwan, R.F. Brazilian kefir: structure, microbial communities and chemical composition. Braz. J. Microbiol., 2011, 42(2), 693-702.
[http://dx.doi.org/10.1590/S1517-83822011000200034] [PMID: 24031681]
[18]
Garofalo, C.; Osimani, A.; Milanović, V.; Aquilanti, L.; De Filippis, F.; Stellato, G.; Di Mauro, S.; Turchetti, B.; Buzzini, P.; Ercolini, D.; Clementi, F. Bacteria and yeast microbiota in milk kefir grains from different Italian regions. Food Microbiol., 2015, 49, 123-133.
[http://dx.doi.org/10.1016/j.fm.2015.01.017] [PMID: 25846922]
[19]
Nalbantoglu, U.; Cakar, A.; Dogan, H.; Abaci, N.; Ustek, D.; Sayood, K.; Can, H. Metagenomic analysis of the microbial community in kefir grains. Food Microbiol., 2014, 41, 42-51.
[http://dx.doi.org/10.1016/j.fm.2014.01.014] [PMID: 24750812]
[20]
Korsak, N.; Taminiau, B.; Leclercq, M.; Nezer, C.; Crevecoeur, S.; Ferauche, C.; Detry, E.; Delcenserie, V.; Daube, G. Short communication: Evaluation of the microbiota of kefir samples using metagenetic analysis targeting the 16S and 26S ribosomal DNA fragments. J. Dairy Sci., 2015, 98(6), 3684-3689.
[http://dx.doi.org/10.3168/jds.2014-9065] [PMID: 25828663]
[21]
Gao, J.; Gu, F.; Abdella, N.H.; Ruan, H.; He, G. Investigation on culturable microflora in Tibetan kefir grains from different areas of China. J. Food Sci., 2012, 77(8), M425-M433.
[http://dx.doi.org/10.1111/j.1750-3841.2012.02805.x] [PMID: 22860591]
[22]
Wang, Y.; Li, C.; Liu, P.; Ahmed, Z.; Xiao, P.; Bai, X. Physical characterization of exopolysaccharide produced by Lactobacillus plantarum KF5 isolated from Tibet Kefir. Carbohydr. Polym., 2010, 82(3), 895-903.
[http://dx.doi.org/10.1016/j.carbpol.2010.06.013]
[23]
Prado, M.R.; Blandón, L.M.; Vandenberghe, L.P.; Rodrigues, C.; Castro, G.R.; Thomaz-Soccol, V.; Soccol, C.R. Milk kefir: composition, microbial cultures, biological activities, and related products. Front. Microbiol., 2015, 6, 1177.
[http://dx.doi.org/10.3389/fmicb.2015.01177] [PMID: 26579086]
[24]
Sharifi, M.; Moridnia, A.; Mortazavi, D.; Salehi, M.; Bagheri, M.; Sheikhi, A. Kefir: a powerful probiotics with anticancer properties. Med. Oncol., 2017, 34(11), 183.
[http://dx.doi.org/10.1007/s12032-017-1044-9] [PMID: 28956261]
[25]
Ghasemlou, M.; Khodaiyan, F.; Jahanbin, K.; Gharibzahedi, S.M.T.; Taheri, S. Structural investigation and response surface optimisation for improvement of kefiran production yield from a low-cost culture medium. Food Chem., 2012, 133(2), 383-389.
[http://dx.doi.org/10.1016/j.foodchem.2012.01.046] [PMID: 25683410]
[26]
Mukai, T.; Toba, T.; Itoh, T.; Adachi, S. Structural investigation of the capsular polysaccharide from Lactobacillus kefiranofaciens K1. Carbohydr. Res., 1990, 204, 227-232.
[http://dx.doi.org/10.1016/0008-6215(90)84039-W] [PMID: 2279248]
[27]
Pogačić, T.; Šinko, S.; Zamberlin, Š.; Samaržija, D. Microbiota of kefir grains. Mljekarstvo, 2013, 63(1), 3-14.
[28]
Piermaria, J.A.; Mariano, L.; Abraham, A.G. Gelling properties of kefiran, a food-grade polysaccharide obtained from kefir grain. Food Hydrocoll., 2008, 22(8), 1520-1527.
[http://dx.doi.org/10.1016/j.foodhyd.2007.10.005]
[29]
Badel, S.; Bernardi, T.; Michaud, P. New perspectives for Lactobacilli exopolysaccharides. Biotechnol. Adv., 2011, 29(1), 54-66.
[http://dx.doi.org/10.1016/j.biotechadv.2010.08.011] [PMID: 20807563]
[30]
Kim, Y.; Ji, U.K.; Oh, S.; Young, J.K.; Kim, M.; Kim, S.H. Technical optimization of culture conditions for the production of exopolysaccharide (EPS) by Lactobacillus rhamnosus ATCC 9595. Food Sci. Biotechnol., 2008, 17(3), 587-593.
[31]
Maeda, H.; Zhu, X.; Omura, K.; Suzuki, S.; Kitamura, S. Effects of an exopolysaccharide (kefiran) on lipids, blood pressure, blood glucose, and constipation. Biofactors, 2004, 22(1-4), 197-200.
[http://dx.doi.org/10.1002/biof.5520220141] [PMID: 15630283]
[32]
Shiomi, M.; Sasaki, K.; Murofushi, M.; Aibara, K. Antitumor activity in mice of orally administered polysaccharide from Kefir grain. Jpn. J. Med. Sci. Biol., 1982, 35(2), 75-80.
[http://dx.doi.org/10.7883/yoken1952.35.75] [PMID: 7109321]
[33]
K.; Magalhães, K. T.; Schwan, R. F. Chemical and therapeutic aspects of kefir. Int. Dairy J., 2003, 13(7), 529-535.
[34]
Yilmaz-Ersan, L.; Ozcan, T.; Akpinar-Bayizit, A.; Sahin, S. The antioxidative capacity of kefir produced from goat milk. Int. J. Chem. Eng. Appl., 2016, 7(1), 22.
[http://dx.doi.org/10.7763/IJCEA.2016.V7.535]
[35]
Piermaria, J.A.; Pinotti, A.; Garcia, M.A.; Abraham, A.G. Films based on kefiran, an exopolysaccharide obtained from kefir grain: Development and characterization. Food Hydrocoll., 2009, 23(3), 684-690.
[http://dx.doi.org/10.1016/j.foodhyd.2008.05.003]
[36]
Wang, M.; Bi, J. Modification of characteristics of kefiran by changing the carbon source of Lactobacillus kefiranofaciens. J. Sci. Food Agric., 2008, 88(5), 763-769.
[http://dx.doi.org/10.1002/jsfa.3136]
[37]
Yeesang, C.; Chanthachum, S.; Cheirsilp, B. Sago starch as a low-cost carbon source for exopolysaccharide production by Lactobacillus kefiranofaciens. World J. Microbiol. Biotechnol., 2008, 24(7), 1195.
[http://dx.doi.org/10.1007/s11274-007-9592-3]
[38]
Cheirsilp, B.; Radchabut, S. Use of whey lactose from dairy industry for economical kefiran production by Lactobacillus kefiranofaciens in mixed cultures with yeasts. N. Biotechnol., 2011, 28(6), 574-580.
[http://dx.doi.org/10.1016/j.nbt.2011.01.009] [PMID: 21315193]
[39]
Tada, S.; Katakura, Y.; Ninomiya, K.; Shioya, S. Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran. J. Biosci. Bioeng., 2007, 103(6), 557-562.
[http://dx.doi.org/10.1263/jbb.103.557] [PMID: 17630128]
[40]
Yokoi, H.; Watanabe, T. Optimum culture conditions for production of kefiran by Lactobacillus sp. KPB-167B isolated from kefir grains. J. Ferment. Bioeng., 1992, 74(5), 327-329.
[http://dx.doi.org/10.1016/0922-338X(92)90069-7]
[41]
Rosa, D.D.; Dias, M.M.S.; Grześkowiak, Ł.M.; Reis, S.A.; Conceição, L.L.; Peluzio, M.D.C.G. Milk kefir: nutritional, microbiological and health benefits. Nutr. Res. Rev., 2017, 30(1), 82-96.
[http://dx.doi.org/10.1017/S0954422416000275] [PMID: 28222814]
[42]
Hong, W.S.; Chen, Y.P.; Chen, M.J. The antiallergic effect of kefir Lactobacilli. J. Food Sci., 2010, 75(8), H244-H253.
[http://dx.doi.org/10.1111/j.1750-3841.2010.01787.x] [PMID: 21535502]
[43]
Ismaiel, A.A.; Ghaly, M.F.; El-Naggar, A.K. Milk kefir: ultrastructure, antimicrobial activity and efficacy on aflatoxin B1 production by Aspergillus flavus. Curr. Microbiol., 2011, 62(5), 1602-1609.
[http://dx.doi.org/10.1007/s00284-011-9901-9] [PMID: 21350802]
[44]
Zheng, Y.; Lu, Y.; Wang, J.; Yang, L.; Pan, C.; Huang, Y. Probiotic properties of Lactobacillus strains isolated from Tibetan kefir grains. PLoS One, 2013, 8(7)e69868
[http://dx.doi.org/10.1371/journal.pone.0069868] [PMID: 23894554]
[45]
Hertzler, S.R.; Clancy, S.M. Kefir improves lactose digestion and tolerance in adults with lactose maldigestion. J. Am. Diet. Assoc., 2003, 103(5), 582-587.
[http://dx.doi.org/10.1053/jada.2003.50111] [PMID: 12728216]
[46]
Farnworth, E.R.; Mainville, I. Kefir—A fermented milk product.Handbook of fermented functional foods; CRC Press, 2008, pp. 89-127.
[http://dx.doi.org/10.1201/9781420053289]
[47]
Dilna, S.V.; Surya, H.; Aswathy, R.G.; Varsha, K.K.; Sakthikumar, D.N.; Pandey, A.; Nampoothiri, K.M. Characterization of an exopolysaccharide with potential health-benefit properties from a probiotic Lactobacillus plantarum RJF4. Lebensm. Wiss. Technol., 2015, 64(2), 1179-1186.
[http://dx.doi.org/10.1016/j.lwt.2015.07.040]
[48]
Zolfi, M.; Khodaiyan, F.; Mousavi, M.; Hashemi, M. Characterization of the new biodegradable WPI/clay nanocomposite films based on kefiran exopolysaccharide. J. Food Sci. Technol., 2015, 52(6), 3485-3493.
[PMID: 26028730]
[49]
London, L.E.; Kumar, A.H.; Wall, R.; Casey, P.G.; O’Sullivan, O.; Shanahan, F.; Hill, C.; Cotter, P.D.; Fitzgerald, G.F.; Ross, R.P.; Caplice, N.M.; Stanton, C. Exopolysaccharide-producing probiotic Lactobacilli reduce serum cholesterol and modify enteric microbiota in ApoE-deficient mice. J. Nutr., 2014, 144(12), 1956-1962.
[http://dx.doi.org/10.3945/jn.114.191627] [PMID: 25320181]
[50]
Wang, Y.; Xu, N.; Xi, A.; Ahmed, Z.; Zhang, B.; Bai, X. Effects of Lactobacillus plantarum MA2 isolated from Tibet kefir on lipid metabolism and intestinal microflora of rats fed on high-cholesterol diet. Appl. Microbiol. Biotechnol., 2009, 84(2), 341-347.
[http://dx.doi.org/10.1007/s00253-009-2012-x] [PMID: 19444443]
[51]
Xiao, J.Z.; Kondo, S.; Takahashi, N.; Miyaji, K.; Oshida, K.; Hiramatsu, A.; Iwatsuki, K.; Kokubo, S.; Hosono, A. Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. J. Dairy Sci., 2003, 86(7), 2452-2461.
[http://dx.doi.org/10.3168/jds.S0022-0302(03)73839-9] [PMID: 12906063]
[52]
Liu, J-R.; Wang, S-Y.; Lin, Y-Y.; Lin, C-W. Antitumor activity of milk kefir and soy milk kefir in tumor-bearing mice. Nutr. Cancer, 2002, 44(2), 183-187.
[http://dx.doi.org/10.1207/S15327914NC4402_10] [PMID: 12734066]
[53]
Elsayed, E.A.; Farooq, M.; Dailin, D.; El-Enshasy, H.A.; Othman, N.Z.; Malek, R.; Danial, E.; Wadaan, M. In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens. Biomed. Res., 2017, 28(2)
[54]
Marquina, D.; Santos, A.; Corpas, I.; Muñoz, J.; Zazo, J.; Peinado, J.M. Dietary influence of kefir on microbial activities in the mouse bowel. Lett. Appl. Microbiol., 2002, 35(2), 136-140.
[http://dx.doi.org/10.1046/j.1472-765X.2002.01155.x] [PMID: 12100589]
[55]
Rodrigues, K.L.; Araújo, T.H.; Schneedorf, J.M.; de Souza Ferreira, C.; Moraes, G.O.I.; Coimbra, R.S.; Rodrigues, M.R. A novel beer fermented by kefir enhances anti-inflammatory and anti-ulcerogenic activities found isolated in its constituents. J. Funct. Foods, 2016, 21, 58-69.
[http://dx.doi.org/10.1016/j.jff.2015.11.035]
[56]
Thoreux, K.; Schmucker, D.L. Kefir milk enhances intestinal immunity in young but not old rats. J. Nutr., 2001, 131(3), 807-812.
[http://dx.doi.org/10.1093/jn/131.3.807] [PMID: 11238763]
[57]
Fard, M.S. Effect of exopolysaccharide extracted from iranian kefir grains on bread quality properties of medium protein wheat. EC Nutrition, 2017, 8, 152-159.
[58]
Soleimanifard, M.; Alami, M.; Najafian, G. Production of kefiran in kefir grains and its effects on the rheological properties low protein wheat dough and quality of France bulky bread. Adv. Crop Sci. Technol., 2015, 3(4), 1-7.
[http://dx.doi.org/10.4172/2329-8863.1000190]
[59]
Rimada, P.S.; Abraham, A.G. Kefiran improves rheological properties of glucono-δ-lactone induced skim milk gels. Int. Dairy J., 2006, 16(1), 33-39.
[http://dx.doi.org/10.1016/j.idairyj.2005.02.002]
[60]
Exarhopoulos, S.; Raphaelides, S.N.; Kontominas, M.G. Flow behavior studies of kefiran systems.Food Hydrocoll.,, 201879, , 282-290.
[http://dx.doi.org/10.1016/j.foodhyd.2017.12.030]

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