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Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

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ISSN (Print): 1871-5230
ISSN (Online): 1875-614X

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Research Article

Identification of Phytoconstituents of Tragia Involucrata leaf Extracts and Evaluate their Correlation with Anti-inflammatory & Antioxidant Properties

Author(s): Vinodhini Velu, Swagata Banerjee, Vidya Radhakrishnan, Gaurav Gupta, Dinesh Kumar Chellappan, Neeraj Kumar Fuloria, Shivkanya Fuloria, Meenu Mehta, Kamal Dua* and Himaja Malipeddi*

Volume 20, Issue 3, 2021

Published on: 26 January, 2021

Page: [308 - 315] Pages: 8

DOI: 10.2174/1871523020666210126144506

Price: $65

Abstract

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts.

Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using Soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be a major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract.

Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28-epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine- 1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87%) and H2O2 method (73.58%) at 200 μg mL-1. Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study.

Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent anti- inflammatory property.

Keywords: Tragia involucrata, terpenoids, antioxidant, anti-inflammatory, GC-MS analysis, molecular docking.

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